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Quantitative determination of cellular [Na(+)] by fluorescence lifetime imaging with CoroNaGreen
Fluorescence lifetime imaging microscopy (FLIM) with fluorescent ion sensors enables the measurement of ion concentrations based on the detection of photon emission events after brief excitation with a pulsed laser source. In contrast to intensity-based imaging, it is independent of dye concentratio...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Rockefeller University Press
2019
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829561/ https://www.ncbi.nlm.nih.gov/pubmed/31597684 http://dx.doi.org/10.1085/jgp.201912404 |