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Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations

To describe the effect of dabigatran on thrombin time (TT) reagents at different concentrations of thrombin. Pooled normal plasma enriched with dabigatran was dissolved in dimethylsulfoxide (DMSO) at concentrations of 0, 20, 50, 100, 200, 300, and 500 ng/mL. Samples with each concentration were eval...

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Detalles Bibliográficos
Autores principales: Xu, Xiaoping, Liang, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829644/
https://www.ncbi.nlm.nih.gov/pubmed/31364394
http://dx.doi.org/10.1177/1076029619867137
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author Xu, Xiaoping
Liang, Qian
author_facet Xu, Xiaoping
Liang, Qian
author_sort Xu, Xiaoping
collection PubMed
description To describe the effect of dabigatran on thrombin time (TT) reagents at different concentrations of thrombin. Pooled normal plasma enriched with dabigatran was dissolved in dimethylsulfoxide (DMSO) at concentrations of 0, 20, 50, 100, 200, 300, and 500 ng/mL. Samples with each concentration were evaluated using a semiautomatic coagulation analyzer to assess the effect of dabigatran on internal normalized ratio (INR), thromboplastin time (APTT), and TT, which were purchased from Instrument Laboratory (IL), Sysmex (SYS), and Stago (STA), respectively. Regarding INR, no reagent showed good sensitivity to increasing concentration of dabigatran, despite all reagents showing good linear response curves (P = .012). Regarding APTT, all reagents had low sensitivity to increasing dabigatran concentration, but SYS-APTT showed a better linear response curve (P = .001). Regarding TT, all reagents had a good linear response to the concentration of dabigatran; however, SYS-TT was very sensitive at low concentrations of dabigatran (0-100 ng/mL), while IL (TT-5 mL) and STA-TT were sensitive at medium concentrations of dabigatran (0-300 ng/mL), and IL (TT-2 mL) was less sensitive for a wide concentration of dabigatran (0-500 ng/mL; P = .007). Internal normalized ratio and APTT showed low sensitivity and SYS-TT showed high sensitivity to concentrations of dabigatran that were unsuitable to monitor. Both IL (TT-5 mL) and STA-TT were useful at medium concentrations of dabigatran by semiautomatic coagulation analyzer, which calculated results using the end point method of coagulation. Instrument Laboratory (TT-2 mL), which contains a higher concentration of thrombin, had better sensitivity to the concentration of dabigatran than APTT and was suitable for routine monitoring by an automatic analyzer.
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spelling pubmed-68296442019-11-07 Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations Xu, Xiaoping Liang, Qian Clin Appl Thromb Hemost Original Article To describe the effect of dabigatran on thrombin time (TT) reagents at different concentrations of thrombin. Pooled normal plasma enriched with dabigatran was dissolved in dimethylsulfoxide (DMSO) at concentrations of 0, 20, 50, 100, 200, 300, and 500 ng/mL. Samples with each concentration were evaluated using a semiautomatic coagulation analyzer to assess the effect of dabigatran on internal normalized ratio (INR), thromboplastin time (APTT), and TT, which were purchased from Instrument Laboratory (IL), Sysmex (SYS), and Stago (STA), respectively. Regarding INR, no reagent showed good sensitivity to increasing concentration of dabigatran, despite all reagents showing good linear response curves (P = .012). Regarding APTT, all reagents had low sensitivity to increasing dabigatran concentration, but SYS-APTT showed a better linear response curve (P = .001). Regarding TT, all reagents had a good linear response to the concentration of dabigatran; however, SYS-TT was very sensitive at low concentrations of dabigatran (0-100 ng/mL), while IL (TT-5 mL) and STA-TT were sensitive at medium concentrations of dabigatran (0-300 ng/mL), and IL (TT-2 mL) was less sensitive for a wide concentration of dabigatran (0-500 ng/mL; P = .007). Internal normalized ratio and APTT showed low sensitivity and SYS-TT showed high sensitivity to concentrations of dabigatran that were unsuitable to monitor. Both IL (TT-5 mL) and STA-TT were useful at medium concentrations of dabigatran by semiautomatic coagulation analyzer, which calculated results using the end point method of coagulation. Instrument Laboratory (TT-2 mL), which contains a higher concentration of thrombin, had better sensitivity to the concentration of dabigatran than APTT and was suitable for routine monitoring by an automatic analyzer. SAGE Publications 2019-07-31 /pmc/articles/PMC6829644/ /pubmed/31364394 http://dx.doi.org/10.1177/1076029619867137 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Article
Xu, Xiaoping
Liang, Qian
Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title_full Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title_fullStr Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title_full_unstemmed Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title_short Dabigatran Monitoring Was Influenced by Thrombin Time Reagent With Different Thrombin Concentrations
title_sort dabigatran monitoring was influenced by thrombin time reagent with different thrombin concentrations
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829644/
https://www.ncbi.nlm.nih.gov/pubmed/31364394
http://dx.doi.org/10.1177/1076029619867137
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