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Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance

During sexual reproduction in eukaryotes, processes such as active degradation and dilution of paternal mitochondria ensure maternal mitochondrial inheritance. In the isogamous organism fission yeast, we employed high-resolution fluorescence microscopy to visualize mitochondrial inheritance during m...

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Autores principales: Chacko, Leeba Ann, Mehta, Kritika, Ananthanarayanan, Vaishnavi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829665/
https://www.ncbi.nlm.nih.gov/pubmed/31582398
http://dx.doi.org/10.1083/jcb.201901108
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author Chacko, Leeba Ann
Mehta, Kritika
Ananthanarayanan, Vaishnavi
author_facet Chacko, Leeba Ann
Mehta, Kritika
Ananthanarayanan, Vaishnavi
author_sort Chacko, Leeba Ann
collection PubMed
description During sexual reproduction in eukaryotes, processes such as active degradation and dilution of paternal mitochondria ensure maternal mitochondrial inheritance. In the isogamous organism fission yeast, we employed high-resolution fluorescence microscopy to visualize mitochondrial inheritance during meiosis by differentially labeling mitochondria of the two parental cells. Remarkably, mitochondria, and thereby mitochondrial DNA from the parental cells, did not mix upon zygote formation but remained segregated at the poles by attaching to clusters of the anchor protein Mcp5 via its coiled-coil domain. We observed that this tethering of parental mitochondria to the poles results in uniparental inheritance of mitochondria, wherein two of the four spores formed subsequently contained mitochondria from one parent and the other spores contained mitochondria from the other parent. Further, the presence of dynein on an Mcp5 cluster precluded the attachment of mitochondria to the same cluster. Taken together, we reveal a distinct mechanism that achieves uniparental inheritance by segregation of parental mitochondria.
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spelling pubmed-68296652019-11-06 Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance Chacko, Leeba Ann Mehta, Kritika Ananthanarayanan, Vaishnavi J Cell Biol Research Articles During sexual reproduction in eukaryotes, processes such as active degradation and dilution of paternal mitochondria ensure maternal mitochondrial inheritance. In the isogamous organism fission yeast, we employed high-resolution fluorescence microscopy to visualize mitochondrial inheritance during meiosis by differentially labeling mitochondria of the two parental cells. Remarkably, mitochondria, and thereby mitochondrial DNA from the parental cells, did not mix upon zygote formation but remained segregated at the poles by attaching to clusters of the anchor protein Mcp5 via its coiled-coil domain. We observed that this tethering of parental mitochondria to the poles results in uniparental inheritance of mitochondria, wherein two of the four spores formed subsequently contained mitochondria from one parent and the other spores contained mitochondria from the other parent. Further, the presence of dynein on an Mcp5 cluster precluded the attachment of mitochondria to the same cluster. Taken together, we reveal a distinct mechanism that achieves uniparental inheritance by segregation of parental mitochondria. Rockefeller University Press 2019-11-04 2019-10-03 /pmc/articles/PMC6829665/ /pubmed/31582398 http://dx.doi.org/10.1083/jcb.201901108 Text en © 2019 Chacko et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Articles
Chacko, Leeba Ann
Mehta, Kritika
Ananthanarayanan, Vaishnavi
Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title_full Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title_fullStr Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title_full_unstemmed Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title_short Cortical tethering of mitochondria by the anchor protein Mcp5 enables uniparental inheritance
title_sort cortical tethering of mitochondria by the anchor protein mcp5 enables uniparental inheritance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829665/
https://www.ncbi.nlm.nih.gov/pubmed/31582398
http://dx.doi.org/10.1083/jcb.201901108
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