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Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice
High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3(−/−) gene knockout mice (Tff3(−/−)/C57BL/6N) have changes in lipid metabolism which may affect v...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829871/ https://www.ncbi.nlm.nih.gov/pubmed/31635131 http://dx.doi.org/10.3390/ijms20205188 |
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author | Kozina, Nataša Mihaljević, Zrinka Baus Lončar, Mirela Mihalj, Martina Mišir, Mihael Radmilović, Marina Dobrivojević Justić, Helena Gajović, Srećko Šešelja, Kate Bazina, Iva Horvatić, Anita Matić, Anita Bijelić, Nikola Rođak, Edi Jukić, Ivana Drenjančević, Ines |
author_facet | Kozina, Nataša Mihaljević, Zrinka Baus Lončar, Mirela Mihalj, Martina Mišir, Mihael Radmilović, Marina Dobrivojević Justić, Helena Gajović, Srećko Šešelja, Kate Bazina, Iva Horvatić, Anita Matić, Anita Bijelić, Nikola Rođak, Edi Jukić, Ivana Drenjančević, Ines |
author_sort | Kozina, Nataša |
collection | PubMed |
description | High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3(−/−) gene knockout mice (Tff3(−/−)/C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3(−/−) and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3(−/−)-HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3(−/−) mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet. |
format | Online Article Text |
id | pubmed-6829871 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68298712019-11-18 Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice Kozina, Nataša Mihaljević, Zrinka Baus Lončar, Mirela Mihalj, Martina Mišir, Mihael Radmilović, Marina Dobrivojević Justić, Helena Gajović, Srećko Šešelja, Kate Bazina, Iva Horvatić, Anita Matić, Anita Bijelić, Nikola Rođak, Edi Jukić, Ivana Drenjančević, Ines Int J Mol Sci Article High salt (HS) dietary intake leads to impaired vascular endothelium-dependent responses to various physiological stimuli, some of which are mediated by arachidonic acid (AA) metabolites. Transgenic Tff3(−/−) gene knockout mice (Tff3(−/−)/C57BL/6N) have changes in lipid metabolism which may affect vascular function and outcomes of stroke. We aimed to study the effects of one week of HS diet (4% NaCl) on vascular function and stroke induced by transient occlusion of middle cerebral artery in Tff3(−/−) and wild type (WT/C57BL/6N) mice. Flow-induced dilation (FID) of carotid artery was reduced in WT-HS mice, but not affected in Tff3(−/−)-HS mice. Nitric oxide (NO) mediated FID. NO production was decreased with HS diet. On the contrary, acetylcholine-induced dilation was significantly decreased in Tff3(−/−) mice on both diets and WT-HS mice. HS intake and Tff3 gene depletion affected the structural components of the vessels. Proteomic analysis revealed a significant effect of Tff3 gene deficiency on HS diet-induced changes in neuronal structural proteins and acute innate immune response proteins’ expression and Tff3 depletion, but HS diet did not increase the stroke volume, which is related to proteome modification and upregulation of genes involved mainly in cellular antioxidative defense. In conclusion, Tff3 depletion seems to partially impair vascular function and worsen the outcomes of stroke, which is moderately affected by HS diet. MDPI 2019-10-19 /pmc/articles/PMC6829871/ /pubmed/31635131 http://dx.doi.org/10.3390/ijms20205188 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kozina, Nataša Mihaljević, Zrinka Baus Lončar, Mirela Mihalj, Martina Mišir, Mihael Radmilović, Marina Dobrivojević Justić, Helena Gajović, Srećko Šešelja, Kate Bazina, Iva Horvatić, Anita Matić, Anita Bijelić, Nikola Rođak, Edi Jukić, Ivana Drenjančević, Ines Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title | Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title_full | Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title_fullStr | Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title_full_unstemmed | Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title_short | Impact of High Salt Diet on Cerebral Vascular Function and Stroke in Tff3(−/−)/C57BL/6N Knockout and WT (C57BL/6N) Control Mice |
title_sort | impact of high salt diet on cerebral vascular function and stroke in tff3(−/−)/c57bl/6n knockout and wt (c57bl/6n) control mice |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6829871/ https://www.ncbi.nlm.nih.gov/pubmed/31635131 http://dx.doi.org/10.3390/ijms20205188 |
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