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Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study
BACKGROUND: Tuberculosis is a serious infection that is common in people living with HIV and increases the mortality and morbidity from the diseases. The study of genetic diversity among strains of M. tuberculosis has a great impact in studying pathogenicity and transmissibility, design for vaccines...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830125/ https://www.ncbi.nlm.nih.gov/pubmed/31720396 http://dx.doi.org/10.1016/j.jctube.2018.06.004 |
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author | Chemeda, Alemu Mihret, Adane Abebe, Tamrat Worku, Adane Ameni, Gobena |
author_facet | Chemeda, Alemu Mihret, Adane Abebe, Tamrat Worku, Adane Ameni, Gobena |
author_sort | Chemeda, Alemu |
collection | PubMed |
description | BACKGROUND: Tuberculosis is a serious infection that is common in people living with HIV and increases the mortality and morbidity from the diseases. The study of genetic diversity among strains of M. tuberculosis has a great impact in studying pathogenicity and transmissibility, design for vaccines production, identification of nominee genes for drug targets, and improving molecular diagnostic techniques. The aim of this study was to characterize Mycobacterium tuberculosis (Mtb) isolated from suspected pulmonary tuberculosis among people living with HIV. METHOD: A total of 143 sputum samples was collected and transported to Akililu Lemma TB laboratory. The collected samples were processed for culture using Lowenstein-Jensen medium. For 45 culture positive isolates, genotyping of mycobacterial DNA was performed by spoligotyping and isolates were assigned to families using the SpolDB4 and the model-based program ‘SPOTCLUST’. Categorical data were analyzed by Chi-square test. RESULT: A high level of diversity was found among the 45 isolates. Twenty six different Spoligo patterns were obtained. The T (46.7%), Family33 (44.4%) and Central Asian (CAS): (4.4%) families were the dominant isolates comprising 91.5% of the total strains. Of 44% of the Euro-American, 6/20(30%) and 9/20(45%), identified were lineage belonged to Spoligo-International-Type (SIT(336)) and SIT(149). Of the total strains, 12 (22%) were unique and have not been described in SpolDB4 to date. CONCLUSION: We found the high diversity of Mtb in pulmonary tuberculosis patients in this setting. T(3_)ETH family identified as the numerous M.tuberculosis strains circulating in the community. |
format | Online Article Text |
id | pubmed-6830125 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68301252019-11-12 Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study Chemeda, Alemu Mihret, Adane Abebe, Tamrat Worku, Adane Ameni, Gobena J Clin Tuberc Other Mycobact Dis Article BACKGROUND: Tuberculosis is a serious infection that is common in people living with HIV and increases the mortality and morbidity from the diseases. The study of genetic diversity among strains of M. tuberculosis has a great impact in studying pathogenicity and transmissibility, design for vaccines production, identification of nominee genes for drug targets, and improving molecular diagnostic techniques. The aim of this study was to characterize Mycobacterium tuberculosis (Mtb) isolated from suspected pulmonary tuberculosis among people living with HIV. METHOD: A total of 143 sputum samples was collected and transported to Akililu Lemma TB laboratory. The collected samples were processed for culture using Lowenstein-Jensen medium. For 45 culture positive isolates, genotyping of mycobacterial DNA was performed by spoligotyping and isolates were assigned to families using the SpolDB4 and the model-based program ‘SPOTCLUST’. Categorical data were analyzed by Chi-square test. RESULT: A high level of diversity was found among the 45 isolates. Twenty six different Spoligo patterns were obtained. The T (46.7%), Family33 (44.4%) and Central Asian (CAS): (4.4%) families were the dominant isolates comprising 91.5% of the total strains. Of 44% of the Euro-American, 6/20(30%) and 9/20(45%), identified were lineage belonged to Spoligo-International-Type (SIT(336)) and SIT(149). Of the total strains, 12 (22%) were unique and have not been described in SpolDB4 to date. CONCLUSION: We found the high diversity of Mtb in pulmonary tuberculosis patients in this setting. T(3_)ETH family identified as the numerous M.tuberculosis strains circulating in the community. Elsevier 2018-06-19 /pmc/articles/PMC6830125/ /pubmed/31720396 http://dx.doi.org/10.1016/j.jctube.2018.06.004 Text en © 2018 Published by Elsevier Ltd. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Chemeda, Alemu Mihret, Adane Abebe, Tamrat Worku, Adane Ameni, Gobena Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title | Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title_full | Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title_fullStr | Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title_full_unstemmed | Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title_short | Genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with HIV in Addis Ababa: Cross-sectional study |
title_sort | genotyping of mycobacterium tuberculosis isolated from pulmonary tuberculosis patients among people living with hiv in addis ababa: cross-sectional study |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830125/ https://www.ncbi.nlm.nih.gov/pubmed/31720396 http://dx.doi.org/10.1016/j.jctube.2018.06.004 |
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