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Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.

Hypericum perforatum L., also known as Saint John’s Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of H. perforatum on infectious bronchitis virus (IBV) were evaluated in vitro and in vivo for the first time. The results...

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Autores principales: Chen, Huijie, Muhammad, Ishfaq, Zhang, Yue, Ren, Yudong, Zhang, Ruili, Huang, Xiaodan, Diao, Lei, Liu, Haixin, Li, Xunliang, Sun, Xiaoqi, Abbas, Ghulam, Li, Guangxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830131/
https://www.ncbi.nlm.nih.gov/pubmed/31736754
http://dx.doi.org/10.3389/fphar.2019.01272
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author Chen, Huijie
Muhammad, Ishfaq
Zhang, Yue
Ren, Yudong
Zhang, Ruili
Huang, Xiaodan
Diao, Lei
Liu, Haixin
Li, Xunliang
Sun, Xiaoqi
Abbas, Ghulam
Li, Guangxing
author_facet Chen, Huijie
Muhammad, Ishfaq
Zhang, Yue
Ren, Yudong
Zhang, Ruili
Huang, Xiaodan
Diao, Lei
Liu, Haixin
Li, Xunliang
Sun, Xiaoqi
Abbas, Ghulam
Li, Guangxing
author_sort Chen, Huijie
collection PubMed
description Hypericum perforatum L., also known as Saint John’s Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of H. perforatum on infectious bronchitis virus (IBV) were evaluated in vitro and in vivo for the first time. The results of in vitro experiments confirmed that the antiviral component of H. perforatum was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative messenger ribonucleic acid (mRNA) expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. HPE treatment at doses of 480–120 mg/kg for 5 days, reduced IBV induced injury in the trachea and kidney, moreover, reduced the mRNA expression level of IBV in the trachea and kidney in vivo. The mRNA expression levels of IL-6, tumor necrosis factor alpha (TNF-α), and nuclear factor kappa beta (NF-κB) significantly decreased, but melanoma differentiation-associated protein 5 (MDA5), mitochondrial antiviral signaling gene, interferon alpha (IFN-α), and interferon beta (IFN-β) mRNA levels significantly increased in vitro and in vivo. Our findings demonstrated that HPE had significant anti-IBV effects in vitro and in vivo, respectively. In addition, it is possible owing to up-regulate mRNA expression of type I interferon through the MDA5 signaling pathway and down-regulate mRNA expression of IL-6 and TNF-α via the NF-κB signaling pathway. Moreover, the mainly active compositions of HPE analyzed by high-performance liquid chromatography/electrospray ionization–mass spectroscopy (ESI-MS) are hyperoside, quercitrin, quercetin, pseudohypericin, and hypericin, and a combination of these compounds could mediate the antiviral activities. This might accelerate our understanding of the antiviral effect of H. perforatum and provide new insights into the development of effective therapeutic strategies.
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spelling pubmed-68301312019-11-15 Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L. Chen, Huijie Muhammad, Ishfaq Zhang, Yue Ren, Yudong Zhang, Ruili Huang, Xiaodan Diao, Lei Liu, Haixin Li, Xunliang Sun, Xiaoqi Abbas, Ghulam Li, Guangxing Front Pharmacol Pharmacology Hypericum perforatum L., also known as Saint John’s Wort, has been well studied for its chemical composition and pharmacological activity. In this study, the antiviral activities of H. perforatum on infectious bronchitis virus (IBV) were evaluated in vitro and in vivo for the first time. The results of in vitro experiments confirmed that the antiviral component of H. perforatum was ethyl acetate extraction section (HPE), and results showed that treatment with HPE significantly reduced the relative messenger ribonucleic acid (mRNA) expression and virus titer of IBV, and reduced positive green immunofluorescence signal of IBV in chicken embryo kidney (CEK) cells. HPE treatment at doses of 480–120 mg/kg for 5 days, reduced IBV induced injury in the trachea and kidney, moreover, reduced the mRNA expression level of IBV in the trachea and kidney in vivo. The mRNA expression levels of IL-6, tumor necrosis factor alpha (TNF-α), and nuclear factor kappa beta (NF-κB) significantly decreased, but melanoma differentiation-associated protein 5 (MDA5), mitochondrial antiviral signaling gene, interferon alpha (IFN-α), and interferon beta (IFN-β) mRNA levels significantly increased in vitro and in vivo. Our findings demonstrated that HPE had significant anti-IBV effects in vitro and in vivo, respectively. In addition, it is possible owing to up-regulate mRNA expression of type I interferon through the MDA5 signaling pathway and down-regulate mRNA expression of IL-6 and TNF-α via the NF-κB signaling pathway. Moreover, the mainly active compositions of HPE analyzed by high-performance liquid chromatography/electrospray ionization–mass spectroscopy (ESI-MS) are hyperoside, quercitrin, quercetin, pseudohypericin, and hypericin, and a combination of these compounds could mediate the antiviral activities. This might accelerate our understanding of the antiviral effect of H. perforatum and provide new insights into the development of effective therapeutic strategies. Frontiers Media S.A. 2019-10-29 /pmc/articles/PMC6830131/ /pubmed/31736754 http://dx.doi.org/10.3389/fphar.2019.01272 Text en Copyright © 2019 Chen, Muhammad, Zhang, Ren, Zhang, Huang, Diao, Liu, Li, Sun, Abbas and Li http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Chen, Huijie
Muhammad, Ishfaq
Zhang, Yue
Ren, Yudong
Zhang, Ruili
Huang, Xiaodan
Diao, Lei
Liu, Haixin
Li, Xunliang
Sun, Xiaoqi
Abbas, Ghulam
Li, Guangxing
Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title_full Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title_fullStr Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title_full_unstemmed Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title_short Antiviral Activity Against Infectious Bronchitis Virus and Bioactive Components of Hypericum perforatum L.
title_sort antiviral activity against infectious bronchitis virus and bioactive components of hypericum perforatum l.
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830131/
https://www.ncbi.nlm.nih.gov/pubmed/31736754
http://dx.doi.org/10.3389/fphar.2019.01272
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