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Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region

Fusidic acid is a steroid antibiotic known since the 1960s. It is frequently used in topical preparations, i.e., ointments, for the treatment of skin and soft tissue infections caused by Staphylococcus aureus. There is an increasing number of methicillin-resistant S. aureus (MRSA) strains that harbo...

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Autores principales: Senok, Abiola, Slickers, Peter, Hotzel, Helmut, Boswihi, Samar, Braun, Sascha D., Gawlik, Darius, Müller, Elke, Nabi, Anju, Nassar, Rania, Nitschke, Hedda, Reissig, Annett, Ruppelt-Lorz, Antje, Mafofo, Joseph, Somily, Ali M., Udo, Edet, Ehricht, Ralf, Monecke, Stefan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830749/
https://www.ncbi.nlm.nih.gov/pubmed/31689288
http://dx.doi.org/10.1371/journal.pone.0223985
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author Senok, Abiola
Slickers, Peter
Hotzel, Helmut
Boswihi, Samar
Braun, Sascha D.
Gawlik, Darius
Müller, Elke
Nabi, Anju
Nassar, Rania
Nitschke, Hedda
Reissig, Annett
Ruppelt-Lorz, Antje
Mafofo, Joseph
Somily, Ali M.
Udo, Edet
Ehricht, Ralf
Monecke, Stefan
author_facet Senok, Abiola
Slickers, Peter
Hotzel, Helmut
Boswihi, Samar
Braun, Sascha D.
Gawlik, Darius
Müller, Elke
Nabi, Anju
Nassar, Rania
Nitschke, Hedda
Reissig, Annett
Ruppelt-Lorz, Antje
Mafofo, Joseph
Somily, Ali M.
Udo, Edet
Ehricht, Ralf
Monecke, Stefan
author_sort Senok, Abiola
collection PubMed
description Fusidic acid is a steroid antibiotic known since the 1960s. It is frequently used in topical preparations, i.e., ointments, for the treatment of skin and soft tissue infections caused by Staphylococcus aureus. There is an increasing number of methicillin-resistant S. aureus (MRSA) strains that harbour plasmid-borne fusB/far1 or fusC that is localised on SCC elements. In this study we examined a series of related CC30-MRSA isolates from the Arabian Gulf countries that presented with SCCmec elements and fusC, including a variant that—to the best of our knowledge—has not yet formally been described. It consisted of a class B mec complex and ccrA/B-4 genes. The fusidic acid resistance gene fusC was present, but contrary to the previously sequenced element of HDE288, it was not accompanied by tirS. This element was identified in CC30 MRSA from Kuwait, Saudi Arabia and the United Arab Emirates that usually also harbour the Panton-Valentin leukocidin (PVL) genes. It was also identified in CC8 and ST834 isolates. In addition, further CC30 MRSA strains with other SCCmec VI elements harbouring fusC were found to circulate in the Arabian Gulf region. It can be assumed that MRSA strains with SCCmec elements that include fusC have a selective advantage in both hospital and community settings warranting a review of the use of topical antibiotics and indicating the necessity of reducing over-the-counter sale of antibiotics, including fusidic acid, without prescription.
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spelling pubmed-68307492019-11-12 Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region Senok, Abiola Slickers, Peter Hotzel, Helmut Boswihi, Samar Braun, Sascha D. Gawlik, Darius Müller, Elke Nabi, Anju Nassar, Rania Nitschke, Hedda Reissig, Annett Ruppelt-Lorz, Antje Mafofo, Joseph Somily, Ali M. Udo, Edet Ehricht, Ralf Monecke, Stefan PLoS One Research Article Fusidic acid is a steroid antibiotic known since the 1960s. It is frequently used in topical preparations, i.e., ointments, for the treatment of skin and soft tissue infections caused by Staphylococcus aureus. There is an increasing number of methicillin-resistant S. aureus (MRSA) strains that harbour plasmid-borne fusB/far1 or fusC that is localised on SCC elements. In this study we examined a series of related CC30-MRSA isolates from the Arabian Gulf countries that presented with SCCmec elements and fusC, including a variant that—to the best of our knowledge—has not yet formally been described. It consisted of a class B mec complex and ccrA/B-4 genes. The fusidic acid resistance gene fusC was present, but contrary to the previously sequenced element of HDE288, it was not accompanied by tirS. This element was identified in CC30 MRSA from Kuwait, Saudi Arabia and the United Arab Emirates that usually also harbour the Panton-Valentin leukocidin (PVL) genes. It was also identified in CC8 and ST834 isolates. In addition, further CC30 MRSA strains with other SCCmec VI elements harbouring fusC were found to circulate in the Arabian Gulf region. It can be assumed that MRSA strains with SCCmec elements that include fusC have a selective advantage in both hospital and community settings warranting a review of the use of topical antibiotics and indicating the necessity of reducing over-the-counter sale of antibiotics, including fusidic acid, without prescription. Public Library of Science 2019-11-05 /pmc/articles/PMC6830749/ /pubmed/31689288 http://dx.doi.org/10.1371/journal.pone.0223985 Text en © 2019 Senok et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Senok, Abiola
Slickers, Peter
Hotzel, Helmut
Boswihi, Samar
Braun, Sascha D.
Gawlik, Darius
Müller, Elke
Nabi, Anju
Nassar, Rania
Nitschke, Hedda
Reissig, Annett
Ruppelt-Lorz, Antje
Mafofo, Joseph
Somily, Ali M.
Udo, Edet
Ehricht, Ralf
Monecke, Stefan
Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title_full Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title_fullStr Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title_full_unstemmed Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title_short Characterisation of a novel SCCmec VI element harbouring fusC in an emerging Staphylococcus aureus strain from the Arabian Gulf region
title_sort characterisation of a novel sccmec vi element harbouring fusc in an emerging staphylococcus aureus strain from the arabian gulf region
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830749/
https://www.ncbi.nlm.nih.gov/pubmed/31689288
http://dx.doi.org/10.1371/journal.pone.0223985
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