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Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection
Background: Urinary tract infection (UTI) is a common disease in sows due to intensification of pig production. Despite direct economic losses, UTI prevalence and respective microbial identification are still poorly studied. Objective: The aims of this study were to identify the causative agents of...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Taylor & Francis
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830825/ https://www.ncbi.nlm.nih.gov/pubmed/29090643 http://dx.doi.org/10.1080/01652176.2017.1397302 |
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author | Moreno, Luisa Z. Matajira, Carlos E.C. Poor, Andre P. Mesquita, Renan E. Gomes, Vasco T.M. Silva, Ana Paula S. Amigo, Cristina R. Christ, Ana Paula G. Barbosa, Mikaela R.F. Sato, Maria Inês Z. Moreno, Andrea M. |
author_facet | Moreno, Luisa Z. Matajira, Carlos E.C. Poor, Andre P. Mesquita, Renan E. Gomes, Vasco T.M. Silva, Ana Paula S. Amigo, Cristina R. Christ, Ana Paula G. Barbosa, Mikaela R.F. Sato, Maria Inês Z. Moreno, Andrea M. |
author_sort | Moreno, Luisa Z. |
collection | PubMed |
description | Background: Urinary tract infection (UTI) is a common disease in sows due to intensification of pig production. Despite direct economic losses, UTI prevalence and respective microbial identification are still poorly studied. Objective: The aims of this study were to identify the causative agents of UTI in sows through MALDI-TOF MS and to characterize their antimicrobial resistance profiles. Materials and Methods: Urine samples from 300 sows of three herds from São Paulo State (Brazil) were screened for UTI; suggestive samples were submitted to bacterial isolation. Species identification was performed by MALDI-TOF MS and susceptibility profiles were determined using disc diffusion method. Results: 128 samples suggestive of UTI were analyzed; 48% of the animals presented UTI caused by a single pathogen, while the remaining 52% presented mixed infection. Escherichia coli stood out with the highest frequency among both single and mixed infections. The Gram-positive were exclusively associated with 27% of single infections. The mixed infections were further classified into 49 profiles. The high frequency of multiresistant profiles stood out for most of the studied isolates. Conclusions: MALDI-TOF MS enabled the identification of rare pathogens related to UTI which may represent higher risk for porcine health, especially considering high frequency of multiresistant profiles. |
format | Online Article Text |
id | pubmed-6830825 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | Taylor & Francis |
record_format | MEDLINE/PubMed |
spelling | pubmed-68308252019-11-19 Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection Moreno, Luisa Z. Matajira, Carlos E.C. Poor, Andre P. Mesquita, Renan E. Gomes, Vasco T.M. Silva, Ana Paula S. Amigo, Cristina R. Christ, Ana Paula G. Barbosa, Mikaela R.F. Sato, Maria Inês Z. Moreno, Andrea M. Vet Q Original Articles Background: Urinary tract infection (UTI) is a common disease in sows due to intensification of pig production. Despite direct economic losses, UTI prevalence and respective microbial identification are still poorly studied. Objective: The aims of this study were to identify the causative agents of UTI in sows through MALDI-TOF MS and to characterize their antimicrobial resistance profiles. Materials and Methods: Urine samples from 300 sows of three herds from São Paulo State (Brazil) were screened for UTI; suggestive samples were submitted to bacterial isolation. Species identification was performed by MALDI-TOF MS and susceptibility profiles were determined using disc diffusion method. Results: 128 samples suggestive of UTI were analyzed; 48% of the animals presented UTI caused by a single pathogen, while the remaining 52% presented mixed infection. Escherichia coli stood out with the highest frequency among both single and mixed infections. The Gram-positive were exclusively associated with 27% of single infections. The mixed infections were further classified into 49 profiles. The high frequency of multiresistant profiles stood out for most of the studied isolates. Conclusions: MALDI-TOF MS enabled the identification of rare pathogens related to UTI which may represent higher risk for porcine health, especially considering high frequency of multiresistant profiles. Taylor & Francis 2017-12-07 /pmc/articles/PMC6830825/ /pubmed/29090643 http://dx.doi.org/10.1080/01652176.2017.1397302 Text en © 2017 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Moreno, Luisa Z. Matajira, Carlos E.C. Poor, Andre P. Mesquita, Renan E. Gomes, Vasco T.M. Silva, Ana Paula S. Amigo, Cristina R. Christ, Ana Paula G. Barbosa, Mikaela R.F. Sato, Maria Inês Z. Moreno, Andrea M. Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title | Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title_full | Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title_fullStr | Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title_full_unstemmed | Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title_short | Identification through MALDI-TOF mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
title_sort | identification through maldi-tof mass spectrometry and antimicrobial susceptibility profiling of bacterial pathogens isolated from sow urinary tract infection |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830825/ https://www.ncbi.nlm.nih.gov/pubmed/29090643 http://dx.doi.org/10.1080/01652176.2017.1397302 |
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