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LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3

This study aimed to explore the role of lncRNA GAS5 in the regulation of the killing effect of NK cells on liver cancer. Compared with a control group, lncRNA GAS5, RUNX3, and NCR1 were down-regulated in NK cells of patients with liver cancer, whereas miR-544 expression was up-regulated in NK cells...

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Autores principales: Fang, Peipei, Xiang, Luxia, Chen, Weilai, Li, Shaoxun, Huang, Shanshan, Li, Jie, Zhuge, Lu, Jin, Lingxiang, Feng, Wenke, Chen, Yiping, Pan, Chenwei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830859/
https://www.ncbi.nlm.nih.gov/pubmed/30774011
http://dx.doi.org/10.1177/1753425919827632
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author Fang, Peipei
Xiang, Luxia
Chen, Weilai
Li, Shaoxun
Huang, Shanshan
Li, Jie
Zhuge, Lu
Jin, Lingxiang
Feng, Wenke
Chen, Yiping
Pan, Chenwei
author_facet Fang, Peipei
Xiang, Luxia
Chen, Weilai
Li, Shaoxun
Huang, Shanshan
Li, Jie
Zhuge, Lu
Jin, Lingxiang
Feng, Wenke
Chen, Yiping
Pan, Chenwei
author_sort Fang, Peipei
collection PubMed
description This study aimed to explore the role of lncRNA GAS5 in the regulation of the killing effect of NK cells on liver cancer. Compared with a control group, lncRNA GAS5, RUNX3, and NCR1 were down-regulated in NK cells of patients with liver cancer, whereas miR-544 expression was up-regulated in NK cells of patients with liver cancer. Activated NK cells had higher IFN-γ level. Knockdown of GAS5 in activated NK cells decreased IFN-γ secretion, NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 and Huh7 cells. We also proved the interaction of GAS5 and miR-544, and the negative regulation role of GAS5 on miR-544. GAS5 overexpression in activated NK cells increased RUNX3 expression, IFN-γ secretion, the NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 cells, while miR-544 mimic abolished the promotion effect of GAS5 overexpression. Finally, in vivo experiments indicated an inhibition effect of GAS5 in tumor growth. LncRNA GAS5 overexpression enhances the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3.
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spelling pubmed-68308592019-11-20 LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3 Fang, Peipei Xiang, Luxia Chen, Weilai Li, Shaoxun Huang, Shanshan Li, Jie Zhuge, Lu Jin, Lingxiang Feng, Wenke Chen, Yiping Pan, Chenwei Innate Immun Original Articles This study aimed to explore the role of lncRNA GAS5 in the regulation of the killing effect of NK cells on liver cancer. Compared with a control group, lncRNA GAS5, RUNX3, and NCR1 were down-regulated in NK cells of patients with liver cancer, whereas miR-544 expression was up-regulated in NK cells of patients with liver cancer. Activated NK cells had higher IFN-γ level. Knockdown of GAS5 in activated NK cells decreased IFN-γ secretion, NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 and Huh7 cells. We also proved the interaction of GAS5 and miR-544, and the negative regulation role of GAS5 on miR-544. GAS5 overexpression in activated NK cells increased RUNX3 expression, IFN-γ secretion, the NK cell cytotoxicity, the percentage of CD107a+ NK cells, and the apoptosis rate of HepG2 cells, while miR-544 mimic abolished the promotion effect of GAS5 overexpression. Finally, in vivo experiments indicated an inhibition effect of GAS5 in tumor growth. LncRNA GAS5 overexpression enhances the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3. SAGE Publications 2019-02-06 2019-02 /pmc/articles/PMC6830859/ /pubmed/30774011 http://dx.doi.org/10.1177/1753425919827632 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Original Articles
Fang, Peipei
Xiang, Luxia
Chen, Weilai
Li, Shaoxun
Huang, Shanshan
Li, Jie
Zhuge, Lu
Jin, Lingxiang
Feng, Wenke
Chen, Yiping
Pan, Chenwei
LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title_full LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title_fullStr LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title_full_unstemmed LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title_short LncRNA GAS5 enhanced the killing effect of NK cell on liver cancer through regulating miR-544/RUNX3
title_sort lncrna gas5 enhanced the killing effect of nk cell on liver cancer through regulating mir-544/runx3
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830859/
https://www.ncbi.nlm.nih.gov/pubmed/30774011
http://dx.doi.org/10.1177/1753425919827632
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