Cargando…

Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations

To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary en...

Descripción completa

Detalles Bibliográficos
Autores principales: Behaegel, Joséphine, Zakaria, Nadia, Tassignon, Marie-José, Leysen, Inge, Bock, Felix, Koppen, Carina, Ní Dhubhghaill, Sorcha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cornea 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830964/
https://www.ncbi.nlm.nih.gov/pubmed/31569145
http://dx.doi.org/10.1097/ICO.0000000000002153
_version_ 1783465867565072384
author Behaegel, Joséphine
Zakaria, Nadia
Tassignon, Marie-José
Leysen, Inge
Bock, Felix
Koppen, Carina
Ní Dhubhghaill, Sorcha
author_facet Behaegel, Joséphine
Zakaria, Nadia
Tassignon, Marie-José
Leysen, Inge
Bock, Felix
Koppen, Carina
Ní Dhubhghaill, Sorcha
author_sort Behaegel, Joséphine
collection PubMed
description To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary end point was to assess the long-term anatomical success rate of transplanted grafts at a follow-up of at least 3 years, in comparison with the short-term outcomes. Secondary end points involved reviewing functional improvement, patient-reported symptoms, and change in percentage area of corneal vascularization in both short-term and long-term. RESULTS: The mean short- and long-term follow-up periods were 2.1 ± 0.38 years and 6.7 ± 1.81 years, respectively. The total anatomical success rate was 46.1% in the short-term, but it decreased to 23.1% in the long-term. A partial success rate of 30.8% was observed in both short- and long-term, and the failure rate increased from 23.1% to 46.1%. The mean percentage of vessel area decreased from 12.11% ± 5.29% preoperatively to 7.82% ± 6.70% in the short-term and increased to 8.70% ± 6.32% in the long-term. There was a significant improvement in best-corrected visual acuity (P = 0.044) in the short-term although not in the long-term (P = 0.865). CONCLUSIONS: This study shows that anatomical and functional success rates of CLET decrease over time. We believe that the decline of success is related to the extent of disease, cell origin, and lack of niche protection because subtotal LSCD and autologous donor cells confer a higher chance of success in the long-term.
format Online
Article
Text
id pubmed-6830964
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Cornea
record_format MEDLINE/PubMed
spelling pubmed-68309642020-01-23 Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations Behaegel, Joséphine Zakaria, Nadia Tassignon, Marie-José Leysen, Inge Bock, Felix Koppen, Carina Ní Dhubhghaill, Sorcha Cornea Clinical Science To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary end point was to assess the long-term anatomical success rate of transplanted grafts at a follow-up of at least 3 years, in comparison with the short-term outcomes. Secondary end points involved reviewing functional improvement, patient-reported symptoms, and change in percentage area of corneal vascularization in both short-term and long-term. RESULTS: The mean short- and long-term follow-up periods were 2.1 ± 0.38 years and 6.7 ± 1.81 years, respectively. The total anatomical success rate was 46.1% in the short-term, but it decreased to 23.1% in the long-term. A partial success rate of 30.8% was observed in both short- and long-term, and the failure rate increased from 23.1% to 46.1%. The mean percentage of vessel area decreased from 12.11% ± 5.29% preoperatively to 7.82% ± 6.70% in the short-term and increased to 8.70% ± 6.32% in the long-term. There was a significant improvement in best-corrected visual acuity (P = 0.044) in the short-term although not in the long-term (P = 0.865). CONCLUSIONS: This study shows that anatomical and functional success rates of CLET decrease over time. We believe that the decline of success is related to the extent of disease, cell origin, and lack of niche protection because subtotal LSCD and autologous donor cells confer a higher chance of success in the long-term. Cornea 2019-09-27 2019-12 /pmc/articles/PMC6830964/ /pubmed/31569145 http://dx.doi.org/10.1097/ICO.0000000000002153 Text en Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.
spellingShingle Clinical Science
Behaegel, Joséphine
Zakaria, Nadia
Tassignon, Marie-José
Leysen, Inge
Bock, Felix
Koppen, Carina
Ní Dhubhghaill, Sorcha
Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title_full Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title_fullStr Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title_full_unstemmed Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title_short Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
title_sort short- and long-term results of xenogeneic-free cultivated autologous and allogeneic limbal epithelial stem cell transplantations
topic Clinical Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830964/
https://www.ncbi.nlm.nih.gov/pubmed/31569145
http://dx.doi.org/10.1097/ICO.0000000000002153
work_keys_str_mv AT behaegeljosephine shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT zakarianadia shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT tassignonmariejose shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT leyseninge shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT bockfelix shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT koppencarina shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations
AT nidhubhghaillsorcha shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations