Cargando…
Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations
To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary en...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cornea
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830964/ https://www.ncbi.nlm.nih.gov/pubmed/31569145 http://dx.doi.org/10.1097/ICO.0000000000002153 |
_version_ | 1783465867565072384 |
---|---|
author | Behaegel, Joséphine Zakaria, Nadia Tassignon, Marie-José Leysen, Inge Bock, Felix Koppen, Carina Ní Dhubhghaill, Sorcha |
author_facet | Behaegel, Joséphine Zakaria, Nadia Tassignon, Marie-José Leysen, Inge Bock, Felix Koppen, Carina Ní Dhubhghaill, Sorcha |
author_sort | Behaegel, Joséphine |
collection | PubMed |
description | To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary end point was to assess the long-term anatomical success rate of transplanted grafts at a follow-up of at least 3 years, in comparison with the short-term outcomes. Secondary end points involved reviewing functional improvement, patient-reported symptoms, and change in percentage area of corneal vascularization in both short-term and long-term. RESULTS: The mean short- and long-term follow-up periods were 2.1 ± 0.38 years and 6.7 ± 1.81 years, respectively. The total anatomical success rate was 46.1% in the short-term, but it decreased to 23.1% in the long-term. A partial success rate of 30.8% was observed in both short- and long-term, and the failure rate increased from 23.1% to 46.1%. The mean percentage of vessel area decreased from 12.11% ± 5.29% preoperatively to 7.82% ± 6.70% in the short-term and increased to 8.70% ± 6.32% in the long-term. There was a significant improvement in best-corrected visual acuity (P = 0.044) in the short-term although not in the long-term (P = 0.865). CONCLUSIONS: This study shows that anatomical and functional success rates of CLET decrease over time. We believe that the decline of success is related to the extent of disease, cell origin, and lack of niche protection because subtotal LSCD and autologous donor cells confer a higher chance of success in the long-term. |
format | Online Article Text |
id | pubmed-6830964 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Cornea |
record_format | MEDLINE/PubMed |
spelling | pubmed-68309642020-01-23 Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations Behaegel, Joséphine Zakaria, Nadia Tassignon, Marie-José Leysen, Inge Bock, Felix Koppen, Carina Ní Dhubhghaill, Sorcha Cornea Clinical Science To evaluate the short- and long-term success rates of xenogeneic-free cultivated limbal epithelial stem cell transplantation (CLET) for the treatment of limbal stem cell deficiency (LSCD). METHODS: Thirteen patients with LSCD underwent an autologous (n = 9) or allogeneic (n = 4) CLET. The primary end point was to assess the long-term anatomical success rate of transplanted grafts at a follow-up of at least 3 years, in comparison with the short-term outcomes. Secondary end points involved reviewing functional improvement, patient-reported symptoms, and change in percentage area of corneal vascularization in both short-term and long-term. RESULTS: The mean short- and long-term follow-up periods were 2.1 ± 0.38 years and 6.7 ± 1.81 years, respectively. The total anatomical success rate was 46.1% in the short-term, but it decreased to 23.1% in the long-term. A partial success rate of 30.8% was observed in both short- and long-term, and the failure rate increased from 23.1% to 46.1%. The mean percentage of vessel area decreased from 12.11% ± 5.29% preoperatively to 7.82% ± 6.70% in the short-term and increased to 8.70% ± 6.32% in the long-term. There was a significant improvement in best-corrected visual acuity (P = 0.044) in the short-term although not in the long-term (P = 0.865). CONCLUSIONS: This study shows that anatomical and functional success rates of CLET decrease over time. We believe that the decline of success is related to the extent of disease, cell origin, and lack of niche protection because subtotal LSCD and autologous donor cells confer a higher chance of success in the long-term. Cornea 2019-09-27 2019-12 /pmc/articles/PMC6830964/ /pubmed/31569145 http://dx.doi.org/10.1097/ICO.0000000000002153 Text en Copyright © 2019 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND) (http://creativecommons.org/licenses/by-nc-nd/4.0/) , where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal. |
spellingShingle | Clinical Science Behaegel, Joséphine Zakaria, Nadia Tassignon, Marie-José Leysen, Inge Bock, Felix Koppen, Carina Ní Dhubhghaill, Sorcha Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title | Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title_full | Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title_fullStr | Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title_full_unstemmed | Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title_short | Short- and Long-Term Results of Xenogeneic-Free Cultivated Autologous and Allogeneic Limbal Epithelial Stem Cell Transplantations |
title_sort | short- and long-term results of xenogeneic-free cultivated autologous and allogeneic limbal epithelial stem cell transplantations |
topic | Clinical Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830964/ https://www.ncbi.nlm.nih.gov/pubmed/31569145 http://dx.doi.org/10.1097/ICO.0000000000002153 |
work_keys_str_mv | AT behaegeljosephine shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT zakarianadia shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT tassignonmariejose shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT leyseninge shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT bockfelix shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT koppencarina shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations AT nidhubhghaillsorcha shortandlongtermresultsofxenogeneicfreecultivatedautologousandallogeneiclimbalepithelialstemcelltransplantations |