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Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil

Background:Edwardsiella tarda infections are frequent causes of severe outbreaks in the fish farming industry besides representing possible zoonotic risks. However, naturally occurring outbreaks that affect various species besides fishes are seldom described. Aim: To report an outbreak of acute mort...

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Autores principales: Miniero Davies, Yamê, Xavier de Oliveira, Maria Gabriela, Paulo Vieira Cunha, Marcos, Soares Franco, Leticia, Pulecio Santos, Sandy Lorena, Zanolli Moreno, Luisa, Túlio de Moura Gomes, Vasco, Zanolli Sato, Maria Inês, Schiavo Nardi, Marcello, Micke Moreno, Andrea, Becker Saidenberg, Andre, Rose Marques de Sá, Lilian, Knöbl, Terezinha
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taylor & Francis 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830998/
https://www.ncbi.nlm.nih.gov/pubmed/30668277
http://dx.doi.org/10.1080/01652176.2018.1540070
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author Miniero Davies, Yamê
Xavier de Oliveira, Maria Gabriela
Paulo Vieira Cunha, Marcos
Soares Franco, Leticia
Pulecio Santos, Sandy Lorena
Zanolli Moreno, Luisa
Túlio de Moura Gomes, Vasco
Zanolli Sato, Maria Inês
Schiavo Nardi, Marcello
Micke Moreno, Andrea
Becker Saidenberg, Andre
Rose Marques de Sá, Lilian
Knöbl, Terezinha
author_facet Miniero Davies, Yamê
Xavier de Oliveira, Maria Gabriela
Paulo Vieira Cunha, Marcos
Soares Franco, Leticia
Pulecio Santos, Sandy Lorena
Zanolli Moreno, Luisa
Túlio de Moura Gomes, Vasco
Zanolli Sato, Maria Inês
Schiavo Nardi, Marcello
Micke Moreno, Andrea
Becker Saidenberg, Andre
Rose Marques de Sá, Lilian
Knöbl, Terezinha
author_sort Miniero Davies, Yamê
collection PubMed
description Background:Edwardsiella tarda infections are frequent causes of severe outbreaks in the fish farming industry besides representing possible zoonotic risks. However, naturally occurring outbreaks that affect various species besides fishes are seldom described. Aim: To report an outbreak of acute mortality caused by E. tarda affecting multiple species that inhabited a natural pond in the state of São Paulo, Brazil. Materials and methods: Three adult tilapias, three Mallard ducks and one Snow egret were necropsied and subjected to further microbiological tests. Gross and microscopic lesions were documented. The antibiotic susceptibility and phylogenetic similarities among fish and avian strains were also determined. The E. tarda species was confirmed through MALDI-TOF, partial sodB sequencing and phylogenetic analysis. Results: Macroscopical findings between the three species included intestinal dilatation, mucosal hyperaemia and mucous to liquid contents. Common histopathology findings included acute enteritis, increased number of intraepithelial lymphocytes with bacteria adhered to the intestinal epithelium and lymphoid depletion in the spleen. E. tarda was isolated from several organs from all affected species. The phylogeny employing amplified fragment length polymorphism (AFLP) of eleven strains revealed high similarity (>90%) among the isolates regardless of the affected species or sampled organs. Ten isolates of E. tarda showed susceptibility to all tested antibiotics. Conclusions:E. tarda was identified as the cause of death of the species examined. Further studies would be necessary to determine the virulence of these strains and the possible risks regarding public health.
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spelling pubmed-68309982019-11-19 Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil Miniero Davies, Yamê Xavier de Oliveira, Maria Gabriela Paulo Vieira Cunha, Marcos Soares Franco, Leticia Pulecio Santos, Sandy Lorena Zanolli Moreno, Luisa Túlio de Moura Gomes, Vasco Zanolli Sato, Maria Inês Schiavo Nardi, Marcello Micke Moreno, Andrea Becker Saidenberg, Andre Rose Marques de Sá, Lilian Knöbl, Terezinha Vet Q Original Article Background:Edwardsiella tarda infections are frequent causes of severe outbreaks in the fish farming industry besides representing possible zoonotic risks. However, naturally occurring outbreaks that affect various species besides fishes are seldom described. Aim: To report an outbreak of acute mortality caused by E. tarda affecting multiple species that inhabited a natural pond in the state of São Paulo, Brazil. Materials and methods: Three adult tilapias, three Mallard ducks and one Snow egret were necropsied and subjected to further microbiological tests. Gross and microscopic lesions were documented. The antibiotic susceptibility and phylogenetic similarities among fish and avian strains were also determined. The E. tarda species was confirmed through MALDI-TOF, partial sodB sequencing and phylogenetic analysis. Results: Macroscopical findings between the three species included intestinal dilatation, mucosal hyperaemia and mucous to liquid contents. Common histopathology findings included acute enteritis, increased number of intraepithelial lymphocytes with bacteria adhered to the intestinal epithelium and lymphoid depletion in the spleen. E. tarda was isolated from several organs from all affected species. The phylogeny employing amplified fragment length polymorphism (AFLP) of eleven strains revealed high similarity (>90%) among the isolates regardless of the affected species or sampled organs. Ten isolates of E. tarda showed susceptibility to all tested antibiotics. Conclusions:E. tarda was identified as the cause of death of the species examined. Further studies would be necessary to determine the virulence of these strains and the possible risks regarding public health. Taylor & Francis 2019-01-22 /pmc/articles/PMC6830998/ /pubmed/30668277 http://dx.doi.org/10.1080/01652176.2018.1540070 Text en © 2018 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Miniero Davies, Yamê
Xavier de Oliveira, Maria Gabriela
Paulo Vieira Cunha, Marcos
Soares Franco, Leticia
Pulecio Santos, Sandy Lorena
Zanolli Moreno, Luisa
Túlio de Moura Gomes, Vasco
Zanolli Sato, Maria Inês
Schiavo Nardi, Marcello
Micke Moreno, Andrea
Becker Saidenberg, Andre
Rose Marques de Sá, Lilian
Knöbl, Terezinha
Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title_full Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title_fullStr Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title_full_unstemmed Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title_short Edwardsiella tarda outbreak affecting fishes and aquatic birds in Brazil
title_sort edwardsiella tarda outbreak affecting fishes and aquatic birds in brazil
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6830998/
https://www.ncbi.nlm.nih.gov/pubmed/30668277
http://dx.doi.org/10.1080/01652176.2018.1540070
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