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AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection

Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further imp...

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Autores principales: Wu, Weijie, Wang, Xun, Shen, Mengfei, Li, Li, Yin, Yue, Shen, Lisong, Wang, Weiwei, Cui, Daxiang, Ni, Jian, Chen, Xiaoyuan, Li, Wanwan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831287/
https://www.ncbi.nlm.nih.gov/pubmed/31695763
http://dx.doi.org/10.7150/thno.36525
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author Wu, Weijie
Wang, Xun
Shen, Mengfei
Li, Li
Yin, Yue
Shen, Lisong
Wang, Weiwei
Cui, Daxiang
Ni, Jian
Chen, Xiaoyuan
Li, Wanwan
author_facet Wu, Weijie
Wang, Xun
Shen, Mengfei
Li, Li
Yin, Yue
Shen, Lisong
Wang, Weiwei
Cui, Daxiang
Ni, Jian
Chen, Xiaoyuan
Li, Wanwan
author_sort Wu, Weijie
collection PubMed
description Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further improvement to meet the increasing demands of “precision diagnosis”. Methods: This work reports a novel suspension array platform based on extremely stable AIEgens (AIE33 and AIE NIR800) microbeads as barcodes and AIEgens (1,1,2,3,4,5-Hexaphenyl-1H-silole, HPS) nanobeads as fluorescent signal reporter coupled with flow cytometry for multiplexed detection. Results: Due to the excellent fluorescent signal amplification effect of the HPS nanobeads, our multiplex assay showed enhanced detection sensitivity, compared to multiplex assay using QDs nanobeads (up to 3-fold improvement) and commercial organic dye of phycoerythrin (up to 5-fold improvement) as the fluorescent signal reporters. Conclusion: Furthermore, validating experiments showed similar detection performance to the clinical gold-standard method of ImmunoCAP for allergen detection in patient serum samples, demonstrating the suspension array platform based on AIEgens microbeads with excellent fluorescence stability and AIEgens nanobeads with strong signal amplification ability is promising for high-sensitivity multiplexed bioassay applications.
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spelling pubmed-68312872019-11-06 AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection Wu, Weijie Wang, Xun Shen, Mengfei Li, Li Yin, Yue Shen, Lisong Wang, Weiwei Cui, Daxiang Ni, Jian Chen, Xiaoyuan Li, Wanwan Theranostics Research Paper Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further improvement to meet the increasing demands of “precision diagnosis”. Methods: This work reports a novel suspension array platform based on extremely stable AIEgens (AIE33 and AIE NIR800) microbeads as barcodes and AIEgens (1,1,2,3,4,5-Hexaphenyl-1H-silole, HPS) nanobeads as fluorescent signal reporter coupled with flow cytometry for multiplexed detection. Results: Due to the excellent fluorescent signal amplification effect of the HPS nanobeads, our multiplex assay showed enhanced detection sensitivity, compared to multiplex assay using QDs nanobeads (up to 3-fold improvement) and commercial organic dye of phycoerythrin (up to 5-fold improvement) as the fluorescent signal reporters. Conclusion: Furthermore, validating experiments showed similar detection performance to the clinical gold-standard method of ImmunoCAP for allergen detection in patient serum samples, demonstrating the suspension array platform based on AIEgens microbeads with excellent fluorescence stability and AIEgens nanobeads with strong signal amplification ability is promising for high-sensitivity multiplexed bioassay applications. Ivyspring International Publisher 2019-09-23 /pmc/articles/PMC6831287/ /pubmed/31695763 http://dx.doi.org/10.7150/thno.36525 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Wu, Weijie
Wang, Xun
Shen, Mengfei
Li, Li
Yin, Yue
Shen, Lisong
Wang, Weiwei
Cui, Daxiang
Ni, Jian
Chen, Xiaoyuan
Li, Wanwan
AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title_full AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title_fullStr AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title_full_unstemmed AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title_short AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
title_sort aiegens barcodes combined with aiegens nanobeads for high-sensitivity multiplexed detection
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831287/
https://www.ncbi.nlm.nih.gov/pubmed/31695763
http://dx.doi.org/10.7150/thno.36525
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