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AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection
Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further imp...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831287/ https://www.ncbi.nlm.nih.gov/pubmed/31695763 http://dx.doi.org/10.7150/thno.36525 |
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author | Wu, Weijie Wang, Xun Shen, Mengfei Li, Li Yin, Yue Shen, Lisong Wang, Weiwei Cui, Daxiang Ni, Jian Chen, Xiaoyuan Li, Wanwan |
author_facet | Wu, Weijie Wang, Xun Shen, Mengfei Li, Li Yin, Yue Shen, Lisong Wang, Weiwei Cui, Daxiang Ni, Jian Chen, Xiaoyuan Li, Wanwan |
author_sort | Wu, Weijie |
collection | PubMed |
description | Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further improvement to meet the increasing demands of “precision diagnosis”. Methods: This work reports a novel suspension array platform based on extremely stable AIEgens (AIE33 and AIE NIR800) microbeads as barcodes and AIEgens (1,1,2,3,4,5-Hexaphenyl-1H-silole, HPS) nanobeads as fluorescent signal reporter coupled with flow cytometry for multiplexed detection. Results: Due to the excellent fluorescent signal amplification effect of the HPS nanobeads, our multiplex assay showed enhanced detection sensitivity, compared to multiplex assay using QDs nanobeads (up to 3-fold improvement) and commercial organic dye of phycoerythrin (up to 5-fold improvement) as the fluorescent signal reporters. Conclusion: Furthermore, validating experiments showed similar detection performance to the clinical gold-standard method of ImmunoCAP for allergen detection in patient serum samples, demonstrating the suspension array platform based on AIEgens microbeads with excellent fluorescence stability and AIEgens nanobeads with strong signal amplification ability is promising for high-sensitivity multiplexed bioassay applications. |
format | Online Article Text |
id | pubmed-6831287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-68312872019-11-06 AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection Wu, Weijie Wang, Xun Shen, Mengfei Li, Li Yin, Yue Shen, Lisong Wang, Weiwei Cui, Daxiang Ni, Jian Chen, Xiaoyuan Li, Wanwan Theranostics Research Paper Suspension arrays based on optical encoded microspheres have attracted great attention for multiplexed detection in gene analysis, protein profiling, early disease diagnosis, treatment monitoring and so on. However, the fluorescence stability of barcodes and detection sensitivity require further improvement to meet the increasing demands of “precision diagnosis”. Methods: This work reports a novel suspension array platform based on extremely stable AIEgens (AIE33 and AIE NIR800) microbeads as barcodes and AIEgens (1,1,2,3,4,5-Hexaphenyl-1H-silole, HPS) nanobeads as fluorescent signal reporter coupled with flow cytometry for multiplexed detection. Results: Due to the excellent fluorescent signal amplification effect of the HPS nanobeads, our multiplex assay showed enhanced detection sensitivity, compared to multiplex assay using QDs nanobeads (up to 3-fold improvement) and commercial organic dye of phycoerythrin (up to 5-fold improvement) as the fluorescent signal reporters. Conclusion: Furthermore, validating experiments showed similar detection performance to the clinical gold-standard method of ImmunoCAP for allergen detection in patient serum samples, demonstrating the suspension array platform based on AIEgens microbeads with excellent fluorescence stability and AIEgens nanobeads with strong signal amplification ability is promising for high-sensitivity multiplexed bioassay applications. Ivyspring International Publisher 2019-09-23 /pmc/articles/PMC6831287/ /pubmed/31695763 http://dx.doi.org/10.7150/thno.36525 Text en © The author(s) This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Wu, Weijie Wang, Xun Shen, Mengfei Li, Li Yin, Yue Shen, Lisong Wang, Weiwei Cui, Daxiang Ni, Jian Chen, Xiaoyuan Li, Wanwan AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title | AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title_full | AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title_fullStr | AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title_full_unstemmed | AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title_short | AIEgens Barcodes Combined with AIEgens Nanobeads for High-sensitivity Multiplexed Detection |
title_sort | aiegens barcodes combined with aiegens nanobeads for high-sensitivity multiplexed detection |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831287/ https://www.ncbi.nlm.nih.gov/pubmed/31695763 http://dx.doi.org/10.7150/thno.36525 |
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