Cargando…

Broad-spectrum resistance to bacterial blight in rice using genome editing

Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transpo...

Descripción completa

Detalles Bibliográficos
Autores principales: Oliva, Ricardo, Ji, Chonghui, Atienza-Grande, Genelou, Huguet-Tapia, José C., Perez-Quintero, Alvaro, Li, Ting, Eom, Joon-Seob, Li, Chenhao, Nguyen, Hanna, Liu, Bo, Auguy, Florence, Sciallano, Coline, Luu, Van T., Dossa, Gerbert S., Cunnac, Sébastien, Schmidt, Sarah M., Slamet-Loedin, Inez H., Vera Cruz, Casiana, Szurek, Boris, Frommer, Wolf B., White, Frank F., Yang, Bing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group US 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831514/
https://www.ncbi.nlm.nih.gov/pubmed/31659337
http://dx.doi.org/10.1038/s41587-019-0267-z
_version_ 1783465986405433344
author Oliva, Ricardo
Ji, Chonghui
Atienza-Grande, Genelou
Huguet-Tapia, José C.
Perez-Quintero, Alvaro
Li, Ting
Eom, Joon-Seob
Li, Chenhao
Nguyen, Hanna
Liu, Bo
Auguy, Florence
Sciallano, Coline
Luu, Van T.
Dossa, Gerbert S.
Cunnac, Sébastien
Schmidt, Sarah M.
Slamet-Loedin, Inez H.
Vera Cruz, Casiana
Szurek, Boris
Frommer, Wolf B.
White, Frank F.
Yang, Bing
author_facet Oliva, Ricardo
Ji, Chonghui
Atienza-Grande, Genelou
Huguet-Tapia, José C.
Perez-Quintero, Alvaro
Li, Ting
Eom, Joon-Seob
Li, Chenhao
Nguyen, Hanna
Liu, Bo
Auguy, Florence
Sciallano, Coline
Luu, Van T.
Dossa, Gerbert S.
Cunnac, Sébastien
Schmidt, Sarah M.
Slamet-Loedin, Inez H.
Vera Cruz, Casiana
Szurek, Boris
Frommer, Wolf B.
White, Frank F.
Yang, Bing
author_sort Oliva, Ricardo
collection PubMed
description Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance.
format Online
Article
Text
id pubmed-6831514
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher Nature Publishing Group US
record_format MEDLINE/PubMed
spelling pubmed-68315142019-11-07 Broad-spectrum resistance to bacterial blight in rice using genome editing Oliva, Ricardo Ji, Chonghui Atienza-Grande, Genelou Huguet-Tapia, José C. Perez-Quintero, Alvaro Li, Ting Eom, Joon-Seob Li, Chenhao Nguyen, Hanna Liu, Bo Auguy, Florence Sciallano, Coline Luu, Van T. Dossa, Gerbert S. Cunnac, Sébastien Schmidt, Sarah M. Slamet-Loedin, Inez H. Vera Cruz, Casiana Szurek, Boris Frommer, Wolf B. White, Frank F. Yang, Bing Nat Biotechnol Article Bacterial blight of rice is an important disease in Asia and Africa. The pathogen, Xanthomonas oryzae pv. oryzae (Xoo), secretes one or more of six known transcription-activator-like effectors (TALes) that bind specific promoter sequences and induce, at minimum, one of the three host sucrose transporter genes SWEET11, SWEET13 and SWEET14, the expression of which is required for disease susceptibility. We used CRISPR–Cas9-mediated genome editing to introduce mutations in all three SWEET gene promoters. Editing was further informed by sequence analyses of TALe genes in 63 Xoo strains, which revealed multiple TALe variants for SWEET13 alleles. Mutations were also created in SWEET14, which is also targeted by two TALes from an African Xoo lineage. A total of five promoter mutations were simultaneously introduced into the rice line Kitaake and the elite mega varieties IR64 and Ciherang-Sub1. Paddy trials showed that genome-edited SWEET promoters endow rice lines with robust, broad-spectrum resistance. Nature Publishing Group US 2019-10-28 2019 /pmc/articles/PMC6831514/ /pubmed/31659337 http://dx.doi.org/10.1038/s41587-019-0267-z Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Oliva, Ricardo
Ji, Chonghui
Atienza-Grande, Genelou
Huguet-Tapia, José C.
Perez-Quintero, Alvaro
Li, Ting
Eom, Joon-Seob
Li, Chenhao
Nguyen, Hanna
Liu, Bo
Auguy, Florence
Sciallano, Coline
Luu, Van T.
Dossa, Gerbert S.
Cunnac, Sébastien
Schmidt, Sarah M.
Slamet-Loedin, Inez H.
Vera Cruz, Casiana
Szurek, Boris
Frommer, Wolf B.
White, Frank F.
Yang, Bing
Broad-spectrum resistance to bacterial blight in rice using genome editing
title Broad-spectrum resistance to bacterial blight in rice using genome editing
title_full Broad-spectrum resistance to bacterial blight in rice using genome editing
title_fullStr Broad-spectrum resistance to bacterial blight in rice using genome editing
title_full_unstemmed Broad-spectrum resistance to bacterial blight in rice using genome editing
title_short Broad-spectrum resistance to bacterial blight in rice using genome editing
title_sort broad-spectrum resistance to bacterial blight in rice using genome editing
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831514/
https://www.ncbi.nlm.nih.gov/pubmed/31659337
http://dx.doi.org/10.1038/s41587-019-0267-z
work_keys_str_mv AT olivaricardo broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT jichonghui broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT atienzagrandegenelou broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT huguettapiajosec broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT perezquinteroalvaro broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT liting broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT eomjoonseob broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT lichenhao broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT nguyenhanna broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT liubo broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT auguyflorence broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT sciallanocoline broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT luuvant broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT dossagerberts broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT cunnacsebastien broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT schmidtsarahm broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT slametloedininezh broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT veracruzcasiana broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT szurekboris broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT frommerwolfb broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT whitefrankf broadspectrumresistancetobacterialblightinriceusinggenomeediting
AT yangbing broadspectrumresistancetobacterialblightinriceusinggenomeediting