Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations

Metallic nanoparticles have unique antimicrobial properties that make them suitable for use within medical and pharmaceutical devices to prevent the spread of infection in healthcare. The use of nanoparticles in healthcare is on the increase with silver being used in many devices. However, not all m...

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Autores principales: Bankier, C., Matharu, R. K., Cheong, Y. K., Ren, G. G., Cloutman-Green, E., Ciric, L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831564/
https://www.ncbi.nlm.nih.gov/pubmed/31690845
http://dx.doi.org/10.1038/s41598-019-52473-2
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author Bankier, C.
Matharu, R. K.
Cheong, Y. K.
Ren, G. G.
Cloutman-Green, E.
Ciric, L.
author_facet Bankier, C.
Matharu, R. K.
Cheong, Y. K.
Ren, G. G.
Cloutman-Green, E.
Ciric, L.
author_sort Bankier, C.
collection PubMed
description Metallic nanoparticles have unique antimicrobial properties that make them suitable for use within medical and pharmaceutical devices to prevent the spread of infection in healthcare. The use of nanoparticles in healthcare is on the increase with silver being used in many devices. However, not all metallic nanoparticles can target and kill all disease-causing bacteria. To overcome this, a combination of several different metallic nanoparticles were used in this study to compare effects of multiple metallic nanoparticles when in combination than when used singly, as single elemental nanoparticles (SENPs), against two common hospital acquired pathogens (Staphylococcus aureus and Pseudomonas. aeruginosa). Flow cytometry LIVE/DEAD assay was used to determine rates of cell death within a bacterial population when exposed to the nanoparticles. Results were analysed using linear models to compare effectiveness of three different metallic nanoparticles, tungsten carbide (WC), silver (Ag) and copper (Cu), in combination and separately. Results show that when the nanoparticles are placed in combination (NPCs), antimicrobial effects significantly increase than when compared with SENPs (P < 0.01). This study demonstrates that certain metallic nanoparticles can be used in combination to improve the antimicrobial efficiency in destroying morphologically distinct pathogens within the healthcare and pharmaceutical industry.
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spelling pubmed-68315642019-11-13 Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations Bankier, C. Matharu, R. K. Cheong, Y. K. Ren, G. G. Cloutman-Green, E. Ciric, L. Sci Rep Article Metallic nanoparticles have unique antimicrobial properties that make them suitable for use within medical and pharmaceutical devices to prevent the spread of infection in healthcare. The use of nanoparticles in healthcare is on the increase with silver being used in many devices. However, not all metallic nanoparticles can target and kill all disease-causing bacteria. To overcome this, a combination of several different metallic nanoparticles were used in this study to compare effects of multiple metallic nanoparticles when in combination than when used singly, as single elemental nanoparticles (SENPs), against two common hospital acquired pathogens (Staphylococcus aureus and Pseudomonas. aeruginosa). Flow cytometry LIVE/DEAD assay was used to determine rates of cell death within a bacterial population when exposed to the nanoparticles. Results were analysed using linear models to compare effectiveness of three different metallic nanoparticles, tungsten carbide (WC), silver (Ag) and copper (Cu), in combination and separately. Results show that when the nanoparticles are placed in combination (NPCs), antimicrobial effects significantly increase than when compared with SENPs (P < 0.01). This study demonstrates that certain metallic nanoparticles can be used in combination to improve the antimicrobial efficiency in destroying morphologically distinct pathogens within the healthcare and pharmaceutical industry. Nature Publishing Group UK 2019-11-05 /pmc/articles/PMC6831564/ /pubmed/31690845 http://dx.doi.org/10.1038/s41598-019-52473-2 Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Bankier, C.
Matharu, R. K.
Cheong, Y. K.
Ren, G. G.
Cloutman-Green, E.
Ciric, L.
Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title_full Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title_fullStr Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title_full_unstemmed Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title_short Synergistic Antibacterial Effects of Metallic Nanoparticle Combinations
title_sort synergistic antibacterial effects of metallic nanoparticle combinations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831564/
https://www.ncbi.nlm.nih.gov/pubmed/31690845
http://dx.doi.org/10.1038/s41598-019-52473-2
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