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Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection
Gold nanoparticles (AuNPs)-based lateral flow strip (LFS) enables a quick screening of foodborne bacteria in point-of-care (POC) diagnostics but suffers from the cumbersome labeling of antibodies and poor sensitive performance. Here, we innovated a label-free immunoassay to detect pathogenic bacteri...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831733/ https://www.ncbi.nlm.nih.gov/pubmed/31701094 http://dx.doi.org/10.1016/j.fochx.2019.100052 |
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author | Bu, Tong Jia, Pei Liu, Jinghan Liu, Yingnan Sun, Xinyu Zhang, Meng Tian, Yongming Zhang, Daohong Wang, Jianlong Wang, Li |
author_facet | Bu, Tong Jia, Pei Liu, Jinghan Liu, Yingnan Sun, Xinyu Zhang, Meng Tian, Yongming Zhang, Daohong Wang, Jianlong Wang, Li |
author_sort | Bu, Tong |
collection | PubMed |
description | Gold nanoparticles (AuNPs)-based lateral flow strip (LFS) enables a quick screening of foodborne bacteria in point-of-care (POC) diagnostics but suffers from the cumbersome labeling of antibodies and poor sensitive performance. Here, we innovated a label-free immunoassay to detect pathogenic bacteria by introducing diversely positive charges functionalized AuNPs ((+) AuNPs). The (+) AuNPs can be loaded on negatively charged bacteria via electrostatic interaction, leading to a color labeling to target bacteria. Afterward, the (+) AuNPs-bacteria complex can be specifically captured by monoclonal antibody (McAb) immobilized on test (T)-line. Under optimum conditions, the proposed LFS exhibited a lowest detectable concentration of 10(3) CFU/mL for Salmonella enteritidis (S. enteritidis) and could be well applied in drinking water, lettuce and pork samples. Moreover, this novel (+) AuNPs-LFS possessed a universal applicability, which could also be used for detecting Escherichia coli O157 (E. coli O157) with a superior sensitivity (10(4) CFU/mL) and specificity. |
format | Online Article Text |
id | pubmed-6831733 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-68317332019-11-07 Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection Bu, Tong Jia, Pei Liu, Jinghan Liu, Yingnan Sun, Xinyu Zhang, Meng Tian, Yongming Zhang, Daohong Wang, Jianlong Wang, Li Food Chem X Article Gold nanoparticles (AuNPs)-based lateral flow strip (LFS) enables a quick screening of foodborne bacteria in point-of-care (POC) diagnostics but suffers from the cumbersome labeling of antibodies and poor sensitive performance. Here, we innovated a label-free immunoassay to detect pathogenic bacteria by introducing diversely positive charges functionalized AuNPs ((+) AuNPs). The (+) AuNPs can be loaded on negatively charged bacteria via electrostatic interaction, leading to a color labeling to target bacteria. Afterward, the (+) AuNPs-bacteria complex can be specifically captured by monoclonal antibody (McAb) immobilized on test (T)-line. Under optimum conditions, the proposed LFS exhibited a lowest detectable concentration of 10(3) CFU/mL for Salmonella enteritidis (S. enteritidis) and could be well applied in drinking water, lettuce and pork samples. Moreover, this novel (+) AuNPs-LFS possessed a universal applicability, which could also be used for detecting Escherichia coli O157 (E. coli O157) with a superior sensitivity (10(4) CFU/mL) and specificity. Elsevier 2019-08-23 /pmc/articles/PMC6831733/ /pubmed/31701094 http://dx.doi.org/10.1016/j.fochx.2019.100052 Text en © 2019 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Bu, Tong Jia, Pei Liu, Jinghan Liu, Yingnan Sun, Xinyu Zhang, Meng Tian, Yongming Zhang, Daohong Wang, Jianlong Wang, Li Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title | Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title_full | Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title_fullStr | Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title_full_unstemmed | Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title_short | Diversely positive-charged gold nanoparticles based biosensor: A label-free and sensitive tool for foodborne pathogen detection |
title_sort | diversely positive-charged gold nanoparticles based biosensor: a label-free and sensitive tool for foodborne pathogen detection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6831733/ https://www.ncbi.nlm.nih.gov/pubmed/31701094 http://dx.doi.org/10.1016/j.fochx.2019.100052 |
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