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In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus

Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to...

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Autores principales: Liu, Panrao, Yin, Yuncong, Gong, Yabin, Qiu, Xusheng, Sun, Yingjie, Tan, Lei, Song, Cuiping, Liu, Weiwei, Liao, Ying, Meng, Chunchun, Ding, Chan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832399/
https://www.ncbi.nlm.nih.gov/pubmed/31635316
http://dx.doi.org/10.3390/v11100962
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author Liu, Panrao
Yin, Yuncong
Gong, Yabin
Qiu, Xusheng
Sun, Yingjie
Tan, Lei
Song, Cuiping
Liu, Weiwei
Liao, Ying
Meng, Chunchun
Ding, Chan
author_facet Liu, Panrao
Yin, Yuncong
Gong, Yabin
Qiu, Xusheng
Sun, Yingjie
Tan, Lei
Song, Cuiping
Liu, Weiwei
Liao, Ying
Meng, Chunchun
Ding, Chan
author_sort Liu, Panrao
collection PubMed
description Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis.
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spelling pubmed-68323992019-11-21 In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus Liu, Panrao Yin, Yuncong Gong, Yabin Qiu, Xusheng Sun, Yingjie Tan, Lei Song, Cuiping Liu, Weiwei Liao, Ying Meng, Chunchun Ding, Chan Viruses Article Newcastle disease (ND) is an acute, febrile, highly contagious disease caused by the virulent Newcastle disease virus (vNDV). The disease causes serious economic losses to the poultry industry. However, the metabolic changes caused by vNDV infection remain unclear. The objective of this study was to determine the metabolomic profiling after infection with vNDV. DF-1 cells infected with the vNDV strain Herts/33 and the lungs from Herts/33-infected specific pathogen-free (SPF) chickens were analyzed via ultra-high-performance liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (UHPLC-QTOF-MS) in combination with multivariate statistical analysis. A total of 305 metabolites were found to have changed significantly after Herts/33 infection, and most of them belong to the amino acid and nucleotide metabolic pathway. It is suggested that the increased pools of amino acids and nucleotides may benefit viral protein synthesis and genome amplification to promote NDV infection. Similar results were also confirmed in vivo. Identification of these metabolites will provide information to further understand the mechanism of vNDV replication and pathogenesis. MDPI 2019-10-18 /pmc/articles/PMC6832399/ /pubmed/31635316 http://dx.doi.org/10.3390/v11100962 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Panrao
Yin, Yuncong
Gong, Yabin
Qiu, Xusheng
Sun, Yingjie
Tan, Lei
Song, Cuiping
Liu, Weiwei
Liao, Ying
Meng, Chunchun
Ding, Chan
In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title_full In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title_fullStr In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title_full_unstemmed In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title_short In Vitro and In Vivo Metabolomic Profiling after Infection with Virulent Newcastle Disease Virus
title_sort in vitro and in vivo metabolomic profiling after infection with virulent newcastle disease virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832399/
https://www.ncbi.nlm.nih.gov/pubmed/31635316
http://dx.doi.org/10.3390/v11100962
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