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High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo

Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in ch...

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Autores principales: Aljabr, Waleed, Armstrong, Stuart, Rickett, Natasha Y., Pollakis, Georgios, Touzelet, Olivier, Cloutman-Green, Elaine, Matthews, David A., Hiscox, Julian A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832471/
https://www.ncbi.nlm.nih.gov/pubmed/31658630
http://dx.doi.org/10.3390/v11100926
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author Aljabr, Waleed
Armstrong, Stuart
Rickett, Natasha Y.
Pollakis, Georgios
Touzelet, Olivier
Cloutman-Green, Elaine
Matthews, David A.
Hiscox, Julian A.
author_facet Aljabr, Waleed
Armstrong, Stuart
Rickett, Natasha Y.
Pollakis, Georgios
Touzelet, Olivier
Cloutman-Green, Elaine
Matthews, David A.
Hiscox, Julian A.
author_sort Aljabr, Waleed
collection PubMed
description Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyngeal aspirates were taken from children with different viral loads and a combined high throughput RNAseq and label free quantitative proteomics approach was used to characterize the nucleic acid and proteins in these samples. HRSV proteins were identified in the nasopharyngeal aspirates from infected children, and their abundance correlated with viral load (Ct value), confirming HRSV infection. Analysis of the HRSV genome indicated that the children were infected with sub-group A virus and that minor variants in nucleotide frequency occurred in discrete clusters along the HRSV genome, and within a patient clustered distinctly within the glycoprotein gene. Data from the samples were binned into four groups; no-HRSV infection (control), high viral load (Ct < 20), medium viral load (Ct = 20–25), and low viral load (Ct > 25). Cellular proteins associated with the anti-viral response (e.g., ISG15) were identified in the nasopharyngeal aspirates and their abundance was correlated with viral load. These combined approaches have not been used before to study HRSV biology in vivo and can be readily applied to the study the variation of virus host interactions.
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spelling pubmed-68324712019-11-25 High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo Aljabr, Waleed Armstrong, Stuart Rickett, Natasha Y. Pollakis, Georgios Touzelet, Olivier Cloutman-Green, Elaine Matthews, David A. Hiscox, Julian A. Viruses Article Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyngeal aspirates were taken from children with different viral loads and a combined high throughput RNAseq and label free quantitative proteomics approach was used to characterize the nucleic acid and proteins in these samples. HRSV proteins were identified in the nasopharyngeal aspirates from infected children, and their abundance correlated with viral load (Ct value), confirming HRSV infection. Analysis of the HRSV genome indicated that the children were infected with sub-group A virus and that minor variants in nucleotide frequency occurred in discrete clusters along the HRSV genome, and within a patient clustered distinctly within the glycoprotein gene. Data from the samples were binned into four groups; no-HRSV infection (control), high viral load (Ct < 20), medium viral load (Ct = 20–25), and low viral load (Ct > 25). Cellular proteins associated with the anti-viral response (e.g., ISG15) were identified in the nasopharyngeal aspirates and their abundance was correlated with viral load. These combined approaches have not been used before to study HRSV biology in vivo and can be readily applied to the study the variation of virus host interactions. MDPI 2019-10-10 /pmc/articles/PMC6832471/ /pubmed/31658630 http://dx.doi.org/10.3390/v11100926 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Aljabr, Waleed
Armstrong, Stuart
Rickett, Natasha Y.
Pollakis, Georgios
Touzelet, Olivier
Cloutman-Green, Elaine
Matthews, David A.
Hiscox, Julian A.
High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title_full High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title_fullStr High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title_full_unstemmed High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title_short High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo
title_sort high resolution analysis of respiratory syncytial virus infection in vivo
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832471/
https://www.ncbi.nlm.nih.gov/pubmed/31658630
http://dx.doi.org/10.3390/v11100926
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