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The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine
Strychnine is an alkaloid with strong toxic properties. Poisoning results in muscular contractions and death through asphyxiation. Intentional or accidental poisonings with strychnine occur mainly in small animals, especially dogs and occasionally cats. Strychnine can be detected in the liver or sto...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832480/ https://www.ncbi.nlm.nih.gov/pubmed/31635042 http://dx.doi.org/10.3390/molecules24203765 |
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author | Śniegocki, Tomasz Sell, Bartosz Posyniak, Andrzej |
author_facet | Śniegocki, Tomasz Sell, Bartosz Posyniak, Andrzej |
author_sort | Śniegocki, Tomasz |
collection | PubMed |
description | Strychnine is an alkaloid with strong toxic properties. Poisoning results in muscular contractions and death through asphyxiation. Intentional or accidental poisonings with strychnine occur mainly in small animals, especially dogs and occasionally cats. Strychnine can be detected in the liver or stomach contents. Unfortunately, the determination of strychnine in these matrices, especially in postmortem examination, is subject to a significant matrix effect that makes it difficult to confirm the presence of the substance being determined. Therefore, we developed a new liquid chromatography method combined with mass spectrometry. One-gram homogenized samples were extracted and partitioned after adding acetonitrile and 5-mol solution of ammonium acetate. After extraction, the samples were analyzed using high-pressure liquid chromatography-MS/MS/MS. The results of validation fulfil the requirement of the confirmatory criteria according to SANTE/11945/2015 regarding apparent recoveries (98.97% to 104.0%), repeatability (2.9%–4.1%), and within-laboratory reproducibility (3.3%–4.6%). The method can be successfully applied to confirm strychnine poisoning cases. |
format | Online Article Text |
id | pubmed-6832480 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68324802019-11-25 The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine Śniegocki, Tomasz Sell, Bartosz Posyniak, Andrzej Molecules Article Strychnine is an alkaloid with strong toxic properties. Poisoning results in muscular contractions and death through asphyxiation. Intentional or accidental poisonings with strychnine occur mainly in small animals, especially dogs and occasionally cats. Strychnine can be detected in the liver or stomach contents. Unfortunately, the determination of strychnine in these matrices, especially in postmortem examination, is subject to a significant matrix effect that makes it difficult to confirm the presence of the substance being determined. Therefore, we developed a new liquid chromatography method combined with mass spectrometry. One-gram homogenized samples were extracted and partitioned after adding acetonitrile and 5-mol solution of ammonium acetate. After extraction, the samples were analyzed using high-pressure liquid chromatography-MS/MS/MS. The results of validation fulfil the requirement of the confirmatory criteria according to SANTE/11945/2015 regarding apparent recoveries (98.97% to 104.0%), repeatability (2.9%–4.1%), and within-laboratory reproducibility (3.3%–4.6%). The method can be successfully applied to confirm strychnine poisoning cases. MDPI 2019-10-19 /pmc/articles/PMC6832480/ /pubmed/31635042 http://dx.doi.org/10.3390/molecules24203765 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Śniegocki, Tomasz Sell, Bartosz Posyniak, Andrzej The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title | The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title_full | The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title_fullStr | The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title_full_unstemmed | The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title_short | The Usefulness of MS(3) to Confirm Poisoning on the Example of Dog Poisoning with Strychnine |
title_sort | usefulness of ms(3) to confirm poisoning on the example of dog poisoning with strychnine |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832480/ https://www.ncbi.nlm.nih.gov/pubmed/31635042 http://dx.doi.org/10.3390/molecules24203765 |
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