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Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)

In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that non...

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Autores principales: Zana, Brigitta, Kemenesi, Gábor, Buzás, Dóra, Csorba, Gábor, Görföl, Tamás, Khan, Faisal Ali Anwarali, Tahir, Nurul Farah Diyana Ahmad, Zeghbib, Safia, Madai, Mónika, Papp, Henrietta, Földes, Fanni, Urbán, Péter, Herczeg, Róbert, Tóth, Gábor Endre, Jakab, Ferenc
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832519/
https://www.ncbi.nlm.nih.gov/pubmed/31546677
http://dx.doi.org/10.3390/v11100887
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author Zana, Brigitta
Kemenesi, Gábor
Buzás, Dóra
Csorba, Gábor
Görföl, Tamás
Khan, Faisal Ali Anwarali
Tahir, Nurul Farah Diyana Ahmad
Zeghbib, Safia
Madai, Mónika
Papp, Henrietta
Földes, Fanni
Urbán, Péter
Herczeg, Róbert
Tóth, Gábor Endre
Jakab, Ferenc
author_facet Zana, Brigitta
Kemenesi, Gábor
Buzás, Dóra
Csorba, Gábor
Görföl, Tamás
Khan, Faisal Ali Anwarali
Tahir, Nurul Farah Diyana Ahmad
Zeghbib, Safia
Madai, Mónika
Papp, Henrietta
Földes, Fanni
Urbán, Péter
Herczeg, Róbert
Tóth, Gábor Endre
Jakab, Ferenc
author_sort Zana, Brigitta
collection PubMed
description In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the Murina aenea bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the M. aenea bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the Mobatvirus genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses.
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spelling pubmed-68325192019-11-25 Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea) Zana, Brigitta Kemenesi, Gábor Buzás, Dóra Csorba, Gábor Görföl, Tamás Khan, Faisal Ali Anwarali Tahir, Nurul Farah Diyana Ahmad Zeghbib, Safia Madai, Mónika Papp, Henrietta Földes, Fanni Urbán, Péter Herczeg, Róbert Tóth, Gábor Endre Jakab, Ferenc Viruses Brief Report In the past ten years, several novel hantaviruses were discovered in shrews, moles, and bats, suggesting the dispersal of hantaviruses in many animal taxa other than rodents during their evolution. Interestingly, the coevolutionary analyses of most recent studies have raised the possibility that nonrodents may have served as the primordial mammalian host and harboured the ancestors of rodent-borne hantaviruses as well. The aim of our study was to investigate the presence of hantaviruses in bat lung tissue homogenates originally collected for taxonomic purposes in Malaysia in 2015. Hantavirus-specific nested RT-PCR screening of 116 samples targeting the L segment of the virus has revealed the positivity of two lung tissue homogenates originating from two individuals, a female and a male of the Murina aenea bat species collected at the same site and sampling occasion. Nanopore sequencing of hantavirus positive samples resulted in partial genomic data from S, M, and L genome segments. The obtained results indicate molecular evidence for hantaviruses in the M. aenea bat species. Sequence analysis of the PCR amplicon and partial genome segments suggests that the identified virus may represent a novel species in the Mobatvirus genus within the Hantaviridae family. Our results provide additional genomic data to help extend our knowledge about the evolution of these viruses. MDPI 2019-09-21 /pmc/articles/PMC6832519/ /pubmed/31546677 http://dx.doi.org/10.3390/v11100887 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Zana, Brigitta
Kemenesi, Gábor
Buzás, Dóra
Csorba, Gábor
Görföl, Tamás
Khan, Faisal Ali Anwarali
Tahir, Nurul Farah Diyana Ahmad
Zeghbib, Safia
Madai, Mónika
Papp, Henrietta
Földes, Fanni
Urbán, Péter
Herczeg, Róbert
Tóth, Gábor Endre
Jakab, Ferenc
Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title_full Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title_fullStr Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title_full_unstemmed Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title_short Molecular Identification of a Novel Hantavirus in Malaysian Bronze Tube-Nosed Bats (Murina aenea)
title_sort molecular identification of a novel hantavirus in malaysian bronze tube-nosed bats (murina aenea)
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832519/
https://www.ncbi.nlm.nih.gov/pubmed/31546677
http://dx.doi.org/10.3390/v11100887
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