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Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice

The demand for licorice and its natural product derivatives in domestic and foreign market is considerably huge. The core production areas of licorice are covered with salinity and drought land in northwestern China. Studies have shown that suitable environmental stress can promote the accumulation...

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Autores principales: Wang, Chengcheng, Chen, Lihong, Cai, Zhichen, Chen, Cuihua, Liu, Zixiu, Liu, Xunhong, Zou, Lisi, Chen, Jiali, Tan, Mengxia, Wei, Lifang, Mei, Yuqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832532/
https://www.ncbi.nlm.nih.gov/pubmed/31614687
http://dx.doi.org/10.3390/molecules24203670
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author Wang, Chengcheng
Chen, Lihong
Cai, Zhichen
Chen, Cuihua
Liu, Zixiu
Liu, Xunhong
Zou, Lisi
Chen, Jiali
Tan, Mengxia
Wei, Lifang
Mei, Yuqi
author_facet Wang, Chengcheng
Chen, Lihong
Cai, Zhichen
Chen, Cuihua
Liu, Zixiu
Liu, Xunhong
Zou, Lisi
Chen, Jiali
Tan, Mengxia
Wei, Lifang
Mei, Yuqi
author_sort Wang, Chengcheng
collection PubMed
description The demand for licorice and its natural product derivatives in domestic and foreign market is considerably huge. The core production areas of licorice are covered with salinity and drought land in northwestern China. Studies have shown that suitable environmental stress can promote the accumulation of glycyrrhizin and liquiritin to improve its quality as medicinal materials. However, there are few reports on other bioactive constituents of licorice, not to mention their dynamic accumulation under stressed conditions. To explore the quality formation of licorice from the perspective of salt influence, a reliable method based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC–MS/MS) was established for simultaneous determination of sixteen bioactive constituents, including triterpenoids, flavonoids, chalcones and their glycosides. Physiological experiments were performed to investigate salt tolerance of licorice under different salinity treatments. The expressions of crucial genes (bAS and CHS), key enzymes of triterpenoid and flavonoid synthesis, were also tested by qRT-PCR. Our study found that 50 mM NaCl treatment (low stress) was the most favorable to promote the accumulation of bioactive constituents in the long term, without harming the plants. Flavonoid accumulation of non-stressed and low-stressed groups became different in the initial synthesis stage, and glycosyltransferases may have great influence on their downstream synthesis. Furthermore, bAS and CHS also showed higher levels in low-stressed licorice at harvest time. This work provides valuable information on dynamic variations in multiple bioactive constituents in licorice treated by salt and insight into its quality formation under stressed conditions.
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spelling pubmed-68325322019-11-25 Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice Wang, Chengcheng Chen, Lihong Cai, Zhichen Chen, Cuihua Liu, Zixiu Liu, Xunhong Zou, Lisi Chen, Jiali Tan, Mengxia Wei, Lifang Mei, Yuqi Molecules Article The demand for licorice and its natural product derivatives in domestic and foreign market is considerably huge. The core production areas of licorice are covered with salinity and drought land in northwestern China. Studies have shown that suitable environmental stress can promote the accumulation of glycyrrhizin and liquiritin to improve its quality as medicinal materials. However, there are few reports on other bioactive constituents of licorice, not to mention their dynamic accumulation under stressed conditions. To explore the quality formation of licorice from the perspective of salt influence, a reliable method based on ultra-fast liquid chromatography tandem triple quadrupole mass spectrometry (UFLC–MS/MS) was established for simultaneous determination of sixteen bioactive constituents, including triterpenoids, flavonoids, chalcones and their glycosides. Physiological experiments were performed to investigate salt tolerance of licorice under different salinity treatments. The expressions of crucial genes (bAS and CHS), key enzymes of triterpenoid and flavonoid synthesis, were also tested by qRT-PCR. Our study found that 50 mM NaCl treatment (low stress) was the most favorable to promote the accumulation of bioactive constituents in the long term, without harming the plants. Flavonoid accumulation of non-stressed and low-stressed groups became different in the initial synthesis stage, and glycosyltransferases may have great influence on their downstream synthesis. Furthermore, bAS and CHS also showed higher levels in low-stressed licorice at harvest time. This work provides valuable information on dynamic variations in multiple bioactive constituents in licorice treated by salt and insight into its quality formation under stressed conditions. MDPI 2019-10-11 /pmc/articles/PMC6832532/ /pubmed/31614687 http://dx.doi.org/10.3390/molecules24203670 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Chengcheng
Chen, Lihong
Cai, Zhichen
Chen, Cuihua
Liu, Zixiu
Liu, Xunhong
Zou, Lisi
Chen, Jiali
Tan, Mengxia
Wei, Lifang
Mei, Yuqi
Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title_full Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title_fullStr Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title_full_unstemmed Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title_short Dynamic Variations in Multiple Bioactive Constituents under Salt Stress Provide Insight into Quality Formation of Licorice
title_sort dynamic variations in multiple bioactive constituents under salt stress provide insight into quality formation of licorice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832532/
https://www.ncbi.nlm.nih.gov/pubmed/31614687
http://dx.doi.org/10.3390/molecules24203670
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