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Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance

In order to remove zearalenone (ZEA) detriment—Bacillus subtilis, Candida utilis, and cell-free extracts from Aspergillus oryzae were used to degrade ZEA in this study. The orthogonal experiment in vitro showed that the ZEA degradation rate was 92.27% (p < 0.05) under the conditions that Candida...

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Autores principales: Liu, Chaoqi, Chang, Juan, Wang, Ping, Yin, Qingqiang, Huang, Weiwei, Dang, Xiaowei, Lu, Fushan, Gao, Tianzeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832534/
https://www.ncbi.nlm.nih.gov/pubmed/31547122
http://dx.doi.org/10.3390/toxins11100552
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author Liu, Chaoqi
Chang, Juan
Wang, Ping
Yin, Qingqiang
Huang, Weiwei
Dang, Xiaowei
Lu, Fushan
Gao, Tianzeng
author_facet Liu, Chaoqi
Chang, Juan
Wang, Ping
Yin, Qingqiang
Huang, Weiwei
Dang, Xiaowei
Lu, Fushan
Gao, Tianzeng
author_sort Liu, Chaoqi
collection PubMed
description In order to remove zearalenone (ZEA) detriment—Bacillus subtilis, Candida utilis, and cell-free extracts from Aspergillus oryzae were used to degrade ZEA in this study. The orthogonal experiment in vitro showed that the ZEA degradation rate was 92.27% (p < 0.05) under the conditions that Candida utilis, Bacillus subtilis SP1, and Bacillus subtilis SP2 were mixed together at 0.5%, 1.0%, and 1.0%. When cell-free extracts from Aspergillus oryzae were combined with the above probiotics at a ratio of 2:1 to make mycotoxin-biodegradation preparation (MBP), the ZEA degradation rate reached 95.15% (p < 0.05). In order to further investigate the MBP effect on relieving the negative impact of ZEA for pig production performance, 120 young pigs were randomly divided into 5 groups, with 3 replicates in each group and 8 pigs for each replicate. Group A was given the basal diet with 86.19 μg/kg ZEA; group B contained 300 μg/kg ZEA without MBP addition; and groups C, D, and E contained 300 μg/kg ZEA added with 0.05%, 0.10%, and 0.15% MBP, respectively. The results showed that MBP addition was able to keep gut microbiota stable. ZEA concentrations in jejunal contents in groups A and D were 89.47% and 80.07% lower than that in group B (p < 0.05), indicating that MBP was effective in ZEA biodegradation. In addition, MBP had no significant effect on pig growth, nutrient digestibility, and the relative mRNA abundance of estrogen receptor alpha (ERα) genes in ovaries and the uterus (p > 0.05).
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spelling pubmed-68325342019-11-25 Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance Liu, Chaoqi Chang, Juan Wang, Ping Yin, Qingqiang Huang, Weiwei Dang, Xiaowei Lu, Fushan Gao, Tianzeng Toxins (Basel) Article In order to remove zearalenone (ZEA) detriment—Bacillus subtilis, Candida utilis, and cell-free extracts from Aspergillus oryzae were used to degrade ZEA in this study. The orthogonal experiment in vitro showed that the ZEA degradation rate was 92.27% (p < 0.05) under the conditions that Candida utilis, Bacillus subtilis SP1, and Bacillus subtilis SP2 were mixed together at 0.5%, 1.0%, and 1.0%. When cell-free extracts from Aspergillus oryzae were combined with the above probiotics at a ratio of 2:1 to make mycotoxin-biodegradation preparation (MBP), the ZEA degradation rate reached 95.15% (p < 0.05). In order to further investigate the MBP effect on relieving the negative impact of ZEA for pig production performance, 120 young pigs were randomly divided into 5 groups, with 3 replicates in each group and 8 pigs for each replicate. Group A was given the basal diet with 86.19 μg/kg ZEA; group B contained 300 μg/kg ZEA without MBP addition; and groups C, D, and E contained 300 μg/kg ZEA added with 0.05%, 0.10%, and 0.15% MBP, respectively. The results showed that MBP addition was able to keep gut microbiota stable. ZEA concentrations in jejunal contents in groups A and D were 89.47% and 80.07% lower than that in group B (p < 0.05), indicating that MBP was effective in ZEA biodegradation. In addition, MBP had no significant effect on pig growth, nutrient digestibility, and the relative mRNA abundance of estrogen receptor alpha (ERα) genes in ovaries and the uterus (p > 0.05). MDPI 2019-09-20 /pmc/articles/PMC6832534/ /pubmed/31547122 http://dx.doi.org/10.3390/toxins11100552 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Chaoqi
Chang, Juan
Wang, Ping
Yin, Qingqiang
Huang, Weiwei
Dang, Xiaowei
Lu, Fushan
Gao, Tianzeng
Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title_full Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title_fullStr Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title_full_unstemmed Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title_short Zearalenone Biodegradation by the Combination of Probiotics with Cell-Free Extracts of Aspergillus oryzae and Its Mycotoxin-Alleviating Effect on Pig Production Performance
title_sort zearalenone biodegradation by the combination of probiotics with cell-free extracts of aspergillus oryzae and its mycotoxin-alleviating effect on pig production performance
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832534/
https://www.ncbi.nlm.nih.gov/pubmed/31547122
http://dx.doi.org/10.3390/toxins11100552
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