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Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus

Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk o...

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Autores principales: Hubert, Mathieu, Chiche, Aurélie, Legros, Vincent, Jeannin, Patricia, Montange, Thomas, Gessain, Antoine, Ceccaldi, Pierre-Emmanuel, Vidy, Aurore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832565/
https://www.ncbi.nlm.nih.gov/pubmed/31619008
http://dx.doi.org/10.3390/v11100950
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author Hubert, Mathieu
Chiche, Aurélie
Legros, Vincent
Jeannin, Patricia
Montange, Thomas
Gessain, Antoine
Ceccaldi, Pierre-Emmanuel
Vidy, Aurore
author_facet Hubert, Mathieu
Chiche, Aurélie
Legros, Vincent
Jeannin, Patricia
Montange, Thomas
Gessain, Antoine
Ceccaldi, Pierre-Emmanuel
Vidy, Aurore
author_sort Hubert, Mathieu
collection PubMed
description Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk of infected mothers, and the existence of breastfeeding as a new mode of mother-to-child transmission of ZIKV was recently hypothesized. However, the maternal origin of infectious particles in breast milk is currently unknown. Here, we show that ZIKV disseminates to the mammary glands of infected mice after both systemic and local exposure with differential kinetics. Ex vivo, we demonstrate that primary human mammary epithelial cells were sensitive and permissive to ZIKV infection in this study. Moreover, by using in vitro models, we prove that mammary luminal- and myoepithelial-phenotype cell lines are both able to produce important virus progeny after ZIKV exposure. Our data suggest that the dissemination of ZIKV to the mammary glands and subsequent infection of the mammary epithelium could be one mechanism of viral excretion in human breast milk.
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spelling pubmed-68325652019-11-25 Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus Hubert, Mathieu Chiche, Aurélie Legros, Vincent Jeannin, Patricia Montange, Thomas Gessain, Antoine Ceccaldi, Pierre-Emmanuel Vidy, Aurore Viruses Article Zika virus (ZIKV) belongs to the large category of arboviruses. Surprisingly, several human-to-human transmissions of ZIKV have been notified, either following sexual intercourse or from the mother to fetus during pregnancy. Importantly, high viral loads have been detected in the human breast milk of infected mothers, and the existence of breastfeeding as a new mode of mother-to-child transmission of ZIKV was recently hypothesized. However, the maternal origin of infectious particles in breast milk is currently unknown. Here, we show that ZIKV disseminates to the mammary glands of infected mice after both systemic and local exposure with differential kinetics. Ex vivo, we demonstrate that primary human mammary epithelial cells were sensitive and permissive to ZIKV infection in this study. Moreover, by using in vitro models, we prove that mammary luminal- and myoepithelial-phenotype cell lines are both able to produce important virus progeny after ZIKV exposure. Our data suggest that the dissemination of ZIKV to the mammary glands and subsequent infection of the mammary epithelium could be one mechanism of viral excretion in human breast milk. MDPI 2019-10-15 /pmc/articles/PMC6832565/ /pubmed/31619008 http://dx.doi.org/10.3390/v11100950 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hubert, Mathieu
Chiche, Aurélie
Legros, Vincent
Jeannin, Patricia
Montange, Thomas
Gessain, Antoine
Ceccaldi, Pierre-Emmanuel
Vidy, Aurore
Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title_full Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title_fullStr Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title_full_unstemmed Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title_short Productive Infection of Mouse Mammary Glands and Human Mammary Epithelial Cells by Zika Virus
title_sort productive infection of mouse mammary glands and human mammary epithelial cells by zika virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832565/
https://www.ncbi.nlm.nih.gov/pubmed/31619008
http://dx.doi.org/10.3390/v11100950
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