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Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD

Endoscopic evaluation is mandatory in establishing the diagnosis of pediatric inflammatory bowel disease (IBD), but unfortunately carries a high burden on patients. Volatile organic compounds (VOC) have been proposed as alternative, noninvasive diagnostic biomarkers for IBD. The current study aimed...

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Autores principales: el Manouni el Hassani, Sofia, Bosch, Sofie, Lemmen, Jesse P.M., Brizzio Brentar, Marina, Ayada, Ibrahim, Wicaksono, Alfian N., Covington, James A., Benninga, Marc A., de Boer, Nanne K.H., de Meij, Tim G.J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832645/
https://www.ncbi.nlm.nih.gov/pubmed/31623289
http://dx.doi.org/10.3390/s19204496
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author el Manouni el Hassani, Sofia
Bosch, Sofie
Lemmen, Jesse P.M.
Brizzio Brentar, Marina
Ayada, Ibrahim
Wicaksono, Alfian N.
Covington, James A.
Benninga, Marc A.
de Boer, Nanne K.H.
de Meij, Tim G.J.
author_facet el Manouni el Hassani, Sofia
Bosch, Sofie
Lemmen, Jesse P.M.
Brizzio Brentar, Marina
Ayada, Ibrahim
Wicaksono, Alfian N.
Covington, James A.
Benninga, Marc A.
de Boer, Nanne K.H.
de Meij, Tim G.J.
author_sort el Manouni el Hassani, Sofia
collection PubMed
description Endoscopic evaluation is mandatory in establishing the diagnosis of pediatric inflammatory bowel disease (IBD), but unfortunately carries a high burden on patients. Volatile organic compounds (VOC) have been proposed as alternative, noninvasive diagnostic biomarkers for IBD. The current study aimed to assess and compare the potential of fecal and urinary VOC as diagnostic biomarkers for pediatric IBD in an intention-to-diagnose cohort. In this cohort study, patients aged 4–17 years, referred to the outpatient clinic of a tertiary referral center under suspicion of IBD, were eligible to participate. The diagnosis was established by endoscopic and histopathologic assessment, participants who did not meet the criteria of IBD were allocated to the control group. Participants were instructed to concurrently collect a fecal and urinary sample prior to bowel lavage. Samples were analyzed by means of gas chromatography–ion mobility spectrometry. In total, five ulcerative colitis patients, five Crohn’s disease patients, and ten age and gender matched controls were included. A significant difference was demonstrated for both fecal (p-value, area under the curve; 0.038, 0.73) and urinary (0.028, 0.78) VOC profiles between IBD and controls. Analysis of both fecal and urinary VOC behold equal potential as noninvasive biomarkers for pediatric IBD diagnosis.
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spelling pubmed-68326452019-11-25 Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD el Manouni el Hassani, Sofia Bosch, Sofie Lemmen, Jesse P.M. Brizzio Brentar, Marina Ayada, Ibrahim Wicaksono, Alfian N. Covington, James A. Benninga, Marc A. de Boer, Nanne K.H. de Meij, Tim G.J. Sensors (Basel) Article Endoscopic evaluation is mandatory in establishing the diagnosis of pediatric inflammatory bowel disease (IBD), but unfortunately carries a high burden on patients. Volatile organic compounds (VOC) have been proposed as alternative, noninvasive diagnostic biomarkers for IBD. The current study aimed to assess and compare the potential of fecal and urinary VOC as diagnostic biomarkers for pediatric IBD in an intention-to-diagnose cohort. In this cohort study, patients aged 4–17 years, referred to the outpatient clinic of a tertiary referral center under suspicion of IBD, were eligible to participate. The diagnosis was established by endoscopic and histopathologic assessment, participants who did not meet the criteria of IBD were allocated to the control group. Participants were instructed to concurrently collect a fecal and urinary sample prior to bowel lavage. Samples were analyzed by means of gas chromatography–ion mobility spectrometry. In total, five ulcerative colitis patients, five Crohn’s disease patients, and ten age and gender matched controls were included. A significant difference was demonstrated for both fecal (p-value, area under the curve; 0.038, 0.73) and urinary (0.028, 0.78) VOC profiles between IBD and controls. Analysis of both fecal and urinary VOC behold equal potential as noninvasive biomarkers for pediatric IBD diagnosis. MDPI 2019-10-16 /pmc/articles/PMC6832645/ /pubmed/31623289 http://dx.doi.org/10.3390/s19204496 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
el Manouni el Hassani, Sofia
Bosch, Sofie
Lemmen, Jesse P.M.
Brizzio Brentar, Marina
Ayada, Ibrahim
Wicaksono, Alfian N.
Covington, James A.
Benninga, Marc A.
de Boer, Nanne K.H.
de Meij, Tim G.J.
Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title_full Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title_fullStr Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title_full_unstemmed Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title_short Simultaneous Assessment of Urinary and Fecal Volatile Organic Compound Analysis in De Novo Pediatric IBD
title_sort simultaneous assessment of urinary and fecal volatile organic compound analysis in de novo pediatric ibd
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832645/
https://www.ncbi.nlm.nih.gov/pubmed/31623289
http://dx.doi.org/10.3390/s19204496
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