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Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers

[Image: see text] Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to...

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Autores principales: Abhyankar, Wishwas R., Zheng, Linli, Brul, Stanley, de Koster, Chris G., de Koning, Leo J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2019
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832669/
https://www.ncbi.nlm.nih.gov/pubmed/31557040
http://dx.doi.org/10.1021/acs.jproteome.9b00413
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author Abhyankar, Wishwas R.
Zheng, Linli
Brul, Stanley
de Koster, Chris G.
de Koning, Leo J.
author_facet Abhyankar, Wishwas R.
Zheng, Linli
Brul, Stanley
de Koster, Chris G.
de Koning, Leo J.
author_sort Abhyankar, Wishwas R.
collection PubMed
description [Image: see text] Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establishing an infection. Bile acids and glycine, both available in sufficient amounts inside the human host intestinal tract, serve as efficient germinants for the spores. It is therefore, for better understanding of C. difficile virulence, crucial to study both the cell and spore states with respect to their genetic, metabolic, and proteomic composition. In the present study, mass spectrometric relative protein quantification, based on the (14)N/(15)N peptide isotopic ratios, has led to quantification of over 700 proteins from combined spore and cell samples. The analysis has revealed that the proteome turnover between a vegetative cell and a spore for this organism is moderate. Additionally, specific cell and spore surface proteins, vegetative cell proteins CD1228, CD3301 and spore proteins CD2487, CD2434, and CD0684 are identified as potential protein markers for C. difficile infection.
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spelling pubmed-68326692019-11-07 Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers Abhyankar, Wishwas R. Zheng, Linli Brul, Stanley de Koster, Chris G. de Koning, Leo J. J Proteome Res [Image: see text] Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establishing an infection. Bile acids and glycine, both available in sufficient amounts inside the human host intestinal tract, serve as efficient germinants for the spores. It is therefore, for better understanding of C. difficile virulence, crucial to study both the cell and spore states with respect to their genetic, metabolic, and proteomic composition. In the present study, mass spectrometric relative protein quantification, based on the (14)N/(15)N peptide isotopic ratios, has led to quantification of over 700 proteins from combined spore and cell samples. The analysis has revealed that the proteome turnover between a vegetative cell and a spore for this organism is moderate. Additionally, specific cell and spore surface proteins, vegetative cell proteins CD1228, CD3301 and spore proteins CD2487, CD2434, and CD0684 are identified as potential protein markers for C. difficile infection. American Chemical Society 2019-09-26 2019-11-01 /pmc/articles/PMC6832669/ /pubmed/31557040 http://dx.doi.org/10.1021/acs.jproteome.9b00413 Text en Copyright © 2019 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Abhyankar, Wishwas R.
Zheng, Linli
Brul, Stanley
de Koster, Chris G.
de Koning, Leo J.
Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title_full Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title_fullStr Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title_full_unstemmed Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title_short Vegetative Cell and Spore Proteomes of Clostridioides difficile Show Finite Differences and Reveal Potential Protein Markers
title_sort vegetative cell and spore proteomes of clostridioides difficile show finite differences and reveal potential protein markers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832669/
https://www.ncbi.nlm.nih.gov/pubmed/31557040
http://dx.doi.org/10.1021/acs.jproteome.9b00413
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