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Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing
Hepatitis delta virus (HDV) RNA forms an unbranched rod-like structure and complexes with the delta antigen (HDAg). Host ADAR1-catalyzed RNA editing at the amber/W site of the small HDAg leads to the production of the large HDAg, which inhibits replication and is required for virion assembly. For HD...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832723/ https://www.ncbi.nlm.nih.gov/pubmed/31614652 http://dx.doi.org/10.3390/v11100934 |
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author | Hsu, Chao-Wei Juang, Horng-Heng Kuo, Chien-Yi Li, Hsin-Pai Iang, Shan-Bei Lin, Siao-Han Yeh, Chau-Ting Chao, Mei |
author_facet | Hsu, Chao-Wei Juang, Horng-Heng Kuo, Chien-Yi Li, Hsin-Pai Iang, Shan-Bei Lin, Siao-Han Yeh, Chau-Ting Chao, Mei |
author_sort | Hsu, Chao-Wei |
collection | PubMed |
description | Hepatitis delta virus (HDV) RNA forms an unbranched rod-like structure and complexes with the delta antigen (HDAg). Host ADAR1-catalyzed RNA editing at the amber/W site of the small HDAg leads to the production of the large HDAg, which inhibits replication and is required for virion assembly. For HDV genotype 1, amber/W editing is controlled by HDAg and the RNA structure immediate vicinity and downstream of the editing site. Here, the effects of 20 mutants carrying an increased length of consecutive base-pairing at various sites in HDV RNA on amber/W site editing were examined. All nine mutants carrying genomic regions that formed up to 15 consecutive base pairs, which is also the maximum length observed in 41 naturally occurring HDV genomes, showed normal editing rate. However, mutants carrying a 16 or 17 consecutive base-paired antigenomic segment located as far as 114 nt upstream could increase editing efficiency, possibly by interfering with HDAg binding. These data show for the first time that extended base-pairing upstream of the amber/W site could increase HDV RNA editing efficiency. Furthermore, it appears that the naturally occurring HDV RNA structures have been selected for suboptimal amber/W RNA editing, which favors the HDV replication cycle. |
format | Online Article Text |
id | pubmed-6832723 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68327232019-11-25 Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing Hsu, Chao-Wei Juang, Horng-Heng Kuo, Chien-Yi Li, Hsin-Pai Iang, Shan-Bei Lin, Siao-Han Yeh, Chau-Ting Chao, Mei Viruses Article Hepatitis delta virus (HDV) RNA forms an unbranched rod-like structure and complexes with the delta antigen (HDAg). Host ADAR1-catalyzed RNA editing at the amber/W site of the small HDAg leads to the production of the large HDAg, which inhibits replication and is required for virion assembly. For HDV genotype 1, amber/W editing is controlled by HDAg and the RNA structure immediate vicinity and downstream of the editing site. Here, the effects of 20 mutants carrying an increased length of consecutive base-pairing at various sites in HDV RNA on amber/W site editing were examined. All nine mutants carrying genomic regions that formed up to 15 consecutive base pairs, which is also the maximum length observed in 41 naturally occurring HDV genomes, showed normal editing rate. However, mutants carrying a 16 or 17 consecutive base-paired antigenomic segment located as far as 114 nt upstream could increase editing efficiency, possibly by interfering with HDAg binding. These data show for the first time that extended base-pairing upstream of the amber/W site could increase HDV RNA editing efficiency. Furthermore, it appears that the naturally occurring HDV RNA structures have been selected for suboptimal amber/W RNA editing, which favors the HDV replication cycle. MDPI 2019-10-11 /pmc/articles/PMC6832723/ /pubmed/31614652 http://dx.doi.org/10.3390/v11100934 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hsu, Chao-Wei Juang, Horng-Heng Kuo, Chien-Yi Li, Hsin-Pai Iang, Shan-Bei Lin, Siao-Han Yeh, Chau-Ting Chao, Mei Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title | Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title_full | Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title_fullStr | Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title_full_unstemmed | Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title_short | Structural Pattern Differences in Unbranched Rod-Like RNA of Hepatitis Delta Virus Affect RNA Editing |
title_sort | structural pattern differences in unbranched rod-like rna of hepatitis delta virus affect rna editing |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832723/ https://www.ncbi.nlm.nih.gov/pubmed/31614652 http://dx.doi.org/10.3390/v11100934 |
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