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Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection
During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chro...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832731/ https://www.ncbi.nlm.nih.gov/pubmed/31614678 http://dx.doi.org/10.3390/v11100935 |
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author | Aho, Vesa Mäntylä, Elina Ekman, Axel Hakanen, Satu Mattola, Salla Chen, Jian-Hua Weinhardt, Venera Ruokolainen, Visa Sodeik, Beate Larabell, Carolyn Vihinen-Ranta, Maija |
author_facet | Aho, Vesa Mäntylä, Elina Ekman, Axel Hakanen, Satu Mattola, Salla Chen, Jian-Hua Weinhardt, Venera Ruokolainen, Visa Sodeik, Beate Larabell, Carolyn Vihinen-Ranta, Maija |
author_sort | Aho, Vesa |
collection | PubMed |
description | During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress sites at the nuclear envelope via interchromatin spaces. |
format | Online Article Text |
id | pubmed-6832731 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68327312019-11-25 Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection Aho, Vesa Mäntylä, Elina Ekman, Axel Hakanen, Satu Mattola, Salla Chen, Jian-Hua Weinhardt, Venera Ruokolainen, Visa Sodeik, Beate Larabell, Carolyn Vihinen-Ranta, Maija Viruses Article During lytic herpes simplex virus 1 (HSV-1) infection, the expansion of the viral replication compartments leads to an enrichment of the host chromatin in the peripheral nucleoplasm. We have shown previously that HSV-1 infection induces the formation of channels through the compacted peripheral chromatin. Here, we used three-dimensional confocal and expansion microscopy, soft X-ray tomography, electron microscopy, and random walk simulations to analyze the kinetics of host chromatin redistribution and capsid localization relative to their egress site at the nuclear envelope. Our data demonstrated a gradual increase in chromatin marginalization, and the kinetics of chromatin smoothening around the viral replication compartments correlated with their expansion. We also observed a gradual transfer of capsids to the nuclear envelope. Later in the infection, random walk modeling indicated a gradually faster transport of capsids to the nuclear envelope that correlated with an increase in the interchromatin channels in the nuclear periphery. Our study reveals a stepwise and time-dependent mechanism of herpesvirus nuclear egress, in which progeny viral capsids approach the egress sites at the nuclear envelope via interchromatin spaces. MDPI 2019-10-11 /pmc/articles/PMC6832731/ /pubmed/31614678 http://dx.doi.org/10.3390/v11100935 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Aho, Vesa Mäntylä, Elina Ekman, Axel Hakanen, Satu Mattola, Salla Chen, Jian-Hua Weinhardt, Venera Ruokolainen, Visa Sodeik, Beate Larabell, Carolyn Vihinen-Ranta, Maija Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title | Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title_full | Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title_fullStr | Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title_full_unstemmed | Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title_short | Quantitative Microscopy Reveals Stepwise Alteration of Chromatin Structure during Herpesvirus Infection |
title_sort | quantitative microscopy reveals stepwise alteration of chromatin structure during herpesvirus infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832731/ https://www.ncbi.nlm.nih.gov/pubmed/31614678 http://dx.doi.org/10.3390/v11100935 |
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