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Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5

Pseudolaric Acid B (PAB), diterpenoid isolated from the root bark of Pseudolarix kaempferi Gordon tree (Pinaceae), exhibits an anti-proliferative and apoptotic activity in various cancer cell lines but to date, the effects of PAB on head and neck cancer (HNC) cell lines remain to be elucidated. In t...

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Autores principales: Choi, Su-Jung, Ahn, Chi-Hyun, Yang, In-Hyoung, Jin, Bohwan, Lee, Won Woo, Kim, Ji-Hoon, Ahn, Min-Hye, Swarup, Neeti, Hong, Kyoung-Ok, Shin, Ji-Ae, Woo, Nam-Tae, Hong, Seong Doo, Lee, Jae-Il, Cho, Sung-Dae
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832876/
https://www.ncbi.nlm.nih.gov/pubmed/31623058
http://dx.doi.org/10.3390/molecules24203715
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author Choi, Su-Jung
Ahn, Chi-Hyun
Yang, In-Hyoung
Jin, Bohwan
Lee, Won Woo
Kim, Ji-Hoon
Ahn, Min-Hye
Swarup, Neeti
Hong, Kyoung-Ok
Shin, Ji-Ae
Woo, Nam-Tae
Hong, Seong Doo
Lee, Jae-Il
Cho, Sung-Dae
author_facet Choi, Su-Jung
Ahn, Chi-Hyun
Yang, In-Hyoung
Jin, Bohwan
Lee, Won Woo
Kim, Ji-Hoon
Ahn, Min-Hye
Swarup, Neeti
Hong, Kyoung-Ok
Shin, Ji-Ae
Woo, Nam-Tae
Hong, Seong Doo
Lee, Jae-Il
Cho, Sung-Dae
author_sort Choi, Su-Jung
collection PubMed
description Pseudolaric Acid B (PAB), diterpenoid isolated from the root bark of Pseudolarix kaempferi Gordon tree (Pinaceae), exhibits an anti-proliferative and apoptotic activity in various cancer cell lines but to date, the effects of PAB on head and neck cancer (HNC) cell lines remain to be elucidated. In this study, we showed that PAB significantly inhibited the viability and caspase-dependent apoptosis in HN22 cell line. PAB-induced apoptosis is through inducing death receptor 5 (DR5) together with the increase in the expression of cleaved caspase-8. It also inhibited the proliferations and induced apoptosis through DR5 in other three HNC cell lines (HSC3, Ca9.22, and HSC4). Extending our in vitro findings, we found that ethanol extract of Pseudolarix kaempferi (2.5 mg/kg/day) reduced tumor growth in a xenograft model bearing HN22 cell line without any change in body weight. DR5 were also found to be increased in tumors tissue of PAB-treated mice without any apparent histopathological changes in liver or kidney tissues. Taken together, PAB may be a potential lead compound for chemotherapeutic agents against head and neck cancer.
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spelling pubmed-68328762019-11-25 Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5 Choi, Su-Jung Ahn, Chi-Hyun Yang, In-Hyoung Jin, Bohwan Lee, Won Woo Kim, Ji-Hoon Ahn, Min-Hye Swarup, Neeti Hong, Kyoung-Ok Shin, Ji-Ae Woo, Nam-Tae Hong, Seong Doo Lee, Jae-Il Cho, Sung-Dae Molecules Article Pseudolaric Acid B (PAB), diterpenoid isolated from the root bark of Pseudolarix kaempferi Gordon tree (Pinaceae), exhibits an anti-proliferative and apoptotic activity in various cancer cell lines but to date, the effects of PAB on head and neck cancer (HNC) cell lines remain to be elucidated. In this study, we showed that PAB significantly inhibited the viability and caspase-dependent apoptosis in HN22 cell line. PAB-induced apoptosis is through inducing death receptor 5 (DR5) together with the increase in the expression of cleaved caspase-8. It also inhibited the proliferations and induced apoptosis through DR5 in other three HNC cell lines (HSC3, Ca9.22, and HSC4). Extending our in vitro findings, we found that ethanol extract of Pseudolarix kaempferi (2.5 mg/kg/day) reduced tumor growth in a xenograft model bearing HN22 cell line without any change in body weight. DR5 were also found to be increased in tumors tissue of PAB-treated mice without any apparent histopathological changes in liver or kidney tissues. Taken together, PAB may be a potential lead compound for chemotherapeutic agents against head and neck cancer. MDPI 2019-10-16 /pmc/articles/PMC6832876/ /pubmed/31623058 http://dx.doi.org/10.3390/molecules24203715 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Choi, Su-Jung
Ahn, Chi-Hyun
Yang, In-Hyoung
Jin, Bohwan
Lee, Won Woo
Kim, Ji-Hoon
Ahn, Min-Hye
Swarup, Neeti
Hong, Kyoung-Ok
Shin, Ji-Ae
Woo, Nam-Tae
Hong, Seong Doo
Lee, Jae-Il
Cho, Sung-Dae
Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title_full Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title_fullStr Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title_full_unstemmed Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title_short Pseudolaric Acid B Induces Growth Inhibition and Caspase-Dependent Apoptosis on Head and Neck Cancer Cell lines through Death Receptor 5
title_sort pseudolaric acid b induces growth inhibition and caspase-dependent apoptosis on head and neck cancer cell lines through death receptor 5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832876/
https://www.ncbi.nlm.nih.gov/pubmed/31623058
http://dx.doi.org/10.3390/molecules24203715
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