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ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage
ε-Polylysine (ε-PL) was studied for the growth inhibition of Shewanella putrefaciens (S. putrefaciens). The minimal inhibitory concentration (MIC) of ε-PL against S. putrefaciens was measured by the broth dilution method, while the membrane permeability and metabolism of S. putrefaciens were assesse...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832906/ https://www.ncbi.nlm.nih.gov/pubmed/31623152 http://dx.doi.org/10.3390/molecules24203727 |
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author | LAN, Weiqing ZHANG, Nannan LIU, Shucheng CHEN, Mengling XIE, Jing |
author_facet | LAN, Weiqing ZHANG, Nannan LIU, Shucheng CHEN, Mengling XIE, Jing |
author_sort | LAN, Weiqing |
collection | PubMed |
description | ε-Polylysine (ε-PL) was studied for the growth inhibition of Shewanella putrefaciens (S. putrefaciens). The minimal inhibitory concentration (MIC) of ε-PL against S. putrefaciens was measured by the broth dilution method, while the membrane permeability and metabolism of S. putrefaciens were assessed after ε-PL treatment. Additionally, growth curves, the content of alkaline phosphatase (AKP), the electrical conductivity (EC), the UV absorbance and scanning electron microscope (SEM) data were used to study cellular morphology. The impact of ε-PL on cell metabolism was also investigated by different methods, such as enzyme activity (peroxidase [POD], catalase [CAT], succinodehydrogenase [SDH] and malic dehydrogenase [MDH]) and cell metabolic activity. The results showed that the MIC of ε-PL against S. putrefaciens was 1.0 mg/mL. When S. putrefaciens was treated with ε-PL, the growth of the bacteria was inhibited and the AKP content, electrical conductivity and UV absorbance were increased, which demonstrated that ε-PL could damage the cell structure. The enzyme activities of POD, CAT, SDH, and MDH in the bacterial solution with ε-PL were decreased compared to those in the ordinary bacterial solution. As the concentration of ε-PL was increased, the enzyme activity decreased further. The respiratory activity of S. putrefaciens was also inhibited by ε-PL. The results suggest that ε-PL acts on the cell membrane of S. putrefaciens, thereby increasing membrane permeability and inhibiting enzyme activity in relation to respiratory metabolism and cell metabolism. This leads to inhibition of cell growth, and eventually cell death. |
format | Online Article Text |
id | pubmed-6832906 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68329062019-11-25 ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage LAN, Weiqing ZHANG, Nannan LIU, Shucheng CHEN, Mengling XIE, Jing Molecules Article ε-Polylysine (ε-PL) was studied for the growth inhibition of Shewanella putrefaciens (S. putrefaciens). The minimal inhibitory concentration (MIC) of ε-PL against S. putrefaciens was measured by the broth dilution method, while the membrane permeability and metabolism of S. putrefaciens were assessed after ε-PL treatment. Additionally, growth curves, the content of alkaline phosphatase (AKP), the electrical conductivity (EC), the UV absorbance and scanning electron microscope (SEM) data were used to study cellular morphology. The impact of ε-PL on cell metabolism was also investigated by different methods, such as enzyme activity (peroxidase [POD], catalase [CAT], succinodehydrogenase [SDH] and malic dehydrogenase [MDH]) and cell metabolic activity. The results showed that the MIC of ε-PL against S. putrefaciens was 1.0 mg/mL. When S. putrefaciens was treated with ε-PL, the growth of the bacteria was inhibited and the AKP content, electrical conductivity and UV absorbance were increased, which demonstrated that ε-PL could damage the cell structure. The enzyme activities of POD, CAT, SDH, and MDH in the bacterial solution with ε-PL were decreased compared to those in the ordinary bacterial solution. As the concentration of ε-PL was increased, the enzyme activity decreased further. The respiratory activity of S. putrefaciens was also inhibited by ε-PL. The results suggest that ε-PL acts on the cell membrane of S. putrefaciens, thereby increasing membrane permeability and inhibiting enzyme activity in relation to respiratory metabolism and cell metabolism. This leads to inhibition of cell growth, and eventually cell death. MDPI 2019-10-16 /pmc/articles/PMC6832906/ /pubmed/31623152 http://dx.doi.org/10.3390/molecules24203727 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article LAN, Weiqing ZHANG, Nannan LIU, Shucheng CHEN, Mengling XIE, Jing ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title | ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title_full | ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title_fullStr | ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title_full_unstemmed | ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title_short | ε-Polylysine Inhibits Shewanella putrefaciens with Membrane Disruption and Cell Damage |
title_sort | ε-polylysine inhibits shewanella putrefaciens with membrane disruption and cell damage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832906/ https://www.ncbi.nlm.nih.gov/pubmed/31623152 http://dx.doi.org/10.3390/molecules24203727 |
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