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Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples
Saxitoxin (STX) and its analogues produced by toxic dinoflagellates accumulate in bivalves, and routine monitoring of bivalves is important to prevent cases of human poisoning. In this study, we describe a rapid detection method for the analysis of STXs using ultra-performance liquid chromatography...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832970/ https://www.ncbi.nlm.nih.gov/pubmed/31581573 http://dx.doi.org/10.3390/toxins11100573 |
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author | Watanabe, Ryuichi Kanamori, Makoto Yoshida, Hidetsugu Okumura, Yutaka Uchida, Hajime Matsushima, Ryoji Oikawa, Hiroshi Suzuki, Toshiyuki |
author_facet | Watanabe, Ryuichi Kanamori, Makoto Yoshida, Hidetsugu Okumura, Yutaka Uchida, Hajime Matsushima, Ryoji Oikawa, Hiroshi Suzuki, Toshiyuki |
author_sort | Watanabe, Ryuichi |
collection | PubMed |
description | Saxitoxin (STX) and its analogues produced by toxic dinoflagellates accumulate in bivalves, and routine monitoring of bivalves is important to prevent cases of human poisoning. In this study, we describe a rapid detection method for the analysis of STXs using ultra-performance liquid chromatography with post-column fluorescent detection and to investigate water depths and sampling points optimal for shellfish toxin monitoring. Cultured scallops (Mizuhopecten yessoensis) and mussels (Mytilus galloprovincialis) collected from various water depths and sampling points were used in this study. Irrespective of bivalve species, toxin concentrations in bivalves were lower at deeper water depths. The toxin concentrations of bivalves did not differ greatly when bivalves were collected from the same bay. Although the levels of contamination of bivalves with STXs can depend on various environmental and geographical factors, our findings are useful for formulating a sampling protocol for the prevention of harvesting contaminated shellfish. |
format | Online Article Text |
id | pubmed-6832970 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68329702019-11-25 Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples Watanabe, Ryuichi Kanamori, Makoto Yoshida, Hidetsugu Okumura, Yutaka Uchida, Hajime Matsushima, Ryoji Oikawa, Hiroshi Suzuki, Toshiyuki Toxins (Basel) Article Saxitoxin (STX) and its analogues produced by toxic dinoflagellates accumulate in bivalves, and routine monitoring of bivalves is important to prevent cases of human poisoning. In this study, we describe a rapid detection method for the analysis of STXs using ultra-performance liquid chromatography with post-column fluorescent detection and to investigate water depths and sampling points optimal for shellfish toxin monitoring. Cultured scallops (Mizuhopecten yessoensis) and mussels (Mytilus galloprovincialis) collected from various water depths and sampling points were used in this study. Irrespective of bivalve species, toxin concentrations in bivalves were lower at deeper water depths. The toxin concentrations of bivalves did not differ greatly when bivalves were collected from the same bay. Although the levels of contamination of bivalves with STXs can depend on various environmental and geographical factors, our findings are useful for formulating a sampling protocol for the prevention of harvesting contaminated shellfish. MDPI 2019-10-01 /pmc/articles/PMC6832970/ /pubmed/31581573 http://dx.doi.org/10.3390/toxins11100573 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Watanabe, Ryuichi Kanamori, Makoto Yoshida, Hidetsugu Okumura, Yutaka Uchida, Hajime Matsushima, Ryoji Oikawa, Hiroshi Suzuki, Toshiyuki Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title | Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title_full | Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title_fullStr | Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title_full_unstemmed | Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title_short | Development of Ultra-Performance Liquid Chromatography with Post-Column Fluorescent Derivatization for the Rapid Detection of Saxitoxin Analogues and Analysis of Bivalve Monitoring Samples |
title_sort | development of ultra-performance liquid chromatography with post-column fluorescent derivatization for the rapid detection of saxitoxin analogues and analysis of bivalve monitoring samples |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6832970/ https://www.ncbi.nlm.nih.gov/pubmed/31581573 http://dx.doi.org/10.3390/toxins11100573 |
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