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Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model

OBJECTIVES: To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model. METHODS: Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disin...

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Autores principales: Huang, Yan, Tang, Xiaoying, Cehreli, Zafer C., Dai, Xiaoyun, Xu, Jiangjingjun, Zhu, Hongshui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: SAGE Publications 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833418/
https://www.ncbi.nlm.nih.gov/pubmed/31364449
http://dx.doi.org/10.1177/0300060519862094
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author Huang, Yan
Tang, Xiaoying
Cehreli, Zafer C.
Dai, Xiaoyun
Xu, Jiangjingjun
Zhu, Hongshui
author_facet Huang, Yan
Tang, Xiaoying
Cehreli, Zafer C.
Dai, Xiaoyun
Xu, Jiangjingjun
Zhu, Hongshui
author_sort Huang, Yan
collection PubMed
description OBJECTIVES: To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model. METHODS: Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration. RESULTS: Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina. CONCLUSIONS: Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth.
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spelling pubmed-68334182019-11-13 Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model Huang, Yan Tang, Xiaoying Cehreli, Zafer C. Dai, Xiaoyun Xu, Jiangjingjun Zhu, Hongshui J Int Med Res Pre-Clinical Research Reports OBJECTIVES: To investigate the potential for pulpal regeneration via autologous transplantation of deciduous tooth pulp into immature necrotic permanent teeth using an experimental dog model. METHODS: Experimental apical periodontitis was induced in 60 teeth of six Beagle dogs. Following canal disinfection and pulpotomy, autologous deciduous pulp tissue was transplanted into the root canals (n = 30); as controls, contralateral teeth were treated in accordance with the recommendations of the American Association of Endodontists. Radiographic examinations were performed immediately before transplant, as well as 3 and 6 months after transplant. At the 6-month examination, root samples were collected and histological and immunohistochemical analyses were used to examine tissue regeneration. RESULTS: Radiographic analysis showed no significant differences in most histopathological parameters examined; however, apical diameter reduction was greater in the experimental group. Histological and immunohistochemical analyses showed that the canal walls of the experimental group had newly formed dentin-like tissue with dentinal tubules, while the control group had cementum-like deposits along the canal wall and apical foramina. CONCLUSIONS: Autologous transplantation may be useful for regeneration of dental pulp in necrotic young permanent teeth. SAGE Publications 2019-07-31 2019-10 /pmc/articles/PMC6833418/ /pubmed/31364449 http://dx.doi.org/10.1177/0300060519862094 Text en © The Author(s) 2019 http://creativecommons.org/licenses/by-nc/4.0/ Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).
spellingShingle Pre-Clinical Research Reports
Huang, Yan
Tang, Xiaoying
Cehreli, Zafer C.
Dai, Xiaoyun
Xu, Jiangjingjun
Zhu, Hongshui
Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title_full Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title_fullStr Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title_full_unstemmed Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title_short Autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
title_sort autologous transplantation of deciduous tooth pulp into necrotic young permanent teeth for pulp regeneration in a dog model
topic Pre-Clinical Research Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6833418/
https://www.ncbi.nlm.nih.gov/pubmed/31364449
http://dx.doi.org/10.1177/0300060519862094
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