Cargando…

Fibrin-Hyaluronic Acid Hydrogel (RegenoGel) with Fibroblast Growth Factor-18 for In Vitro 3D Culture of Human and Bovine Nucleus Pulposus Cells

We investigated the effects of a fibrin-hyaluronic acid hydrogel (FBG–HA) and fibroblast growth factor 18 (FGF-18) for nucleus pulposus (NP) regeneration. Healthy bovine (n = 4) and human degenerated NP cells (n = 4) were cultured for 14 days in FBG-HA hydrogel with FGF-18 (∆51-mutant or wild-type)...

Descripción completa

Detalles Bibliográficos
Autores principales:	Häckel, Sonja, Zolfaghar, Mona, Du, Jie, Hoppe, Sven, Benneker, Lorin M., Garstka, Nathalie, Peroglio, Marianna, Alini, Mauro, Grad, Sibylle, Yayon, Avner, Li, Zhen
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834142/ https://www.ncbi.nlm.nih.gov/pubmed/31614494 http://dx.doi.org/10.3390/ijms20205036

Ejemplares similares

Intervertebral disc response to stem cell treatment is conditioned by disc state and cell carrier: An ex vivo study
por: Peroglio, Marianna, et al.
Publicado: (2017)

Mesenchymal Stem Cell Homing Into Intervertebral Discs Enhances the Tie2-positive Progenitor Cell Population, Prevents Cell Death, and Induces a Proliferative Response
por: Wangler, Sebastian, et al.
Publicado: (2019)

In Vitro Characterization of a Tissue Renin-Angiotensin System in Human Nucleus Pulposus Cells
por: Saravi, Babak, et al.
Publicado: (2022)

Uncovering the secretome of mesenchymal stromal cells exposed to healthy, traumatic, and degenerative intervertebral discs: a proteomic analysis
por: Wangler, Sebastian, et al.
Publicado: (2021)

Incorporation of Collagen and Hyaluronic Acid to Enhance the Bioactivity of Fibrin-Based Hydrogels for Nucleus Pulposus Regeneration
por: Gansau, Jennifer, et al.
Publicado: (2018)

Mechanical Stress Inhibits Early Stages of Endogenous Cell Migration: A Pilot Study in an Ex Vivo Osteochondral Model
por: Vainieri, Maria L., et al.
Publicado: (2020)

Direct and Intervertebral DiscMediated Sensitization of Dorsal Root Ganglion Neurons by Hypoxia and Low pH
por: Ma, Junxuan, et al.
Publicado: (2020)

Exhaustion of nucleus pulposus progenitor cells with ageing and degeneration of the intervertebral disc
por: Sakai, Daisuke, et al.
Publicado: (2012)

Isolation of high‐quality RNA from intervertebral disc tissue via pronase predigestion and tissue pulverization
por: Caprez, Stephanie, et al.
Publicado: (2018)

The influence of intervertebral disc overloading on nociceptor calcium flickering
por: Gewiess, Jan, et al.
Publicado: (2023)

Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
por: Tekari, Adel, et al.
Publicado: (2016)

Correction to: Angiopoietin-1 receptor Tie2 distinguishes multipotent differentiation capability in bovine coccygeal nucleus pulposus cells
por: Tekari, Adel, et al.
Publicado: (2019)

BMP-2 and BMP-2/7 Heterodimers Conjugated to a Fibrin/Hyaluronic Acid Hydrogel in a Large Animal Model of Mild Intervertebral Disc Degeneration
por: Peeters, Mirte, et al.
Publicado: (2015)

The effects of 3D culture on the expansion and maintenance of nucleus pulposus progenitor cell multipotency
por: Guerrero, Julien, et al.
Publicado: (2020)

In Vitro Model to Investigate Communication between Dorsal Root Ganglion and Spinal Cord Glia
por: Ma, Junxuan, et al.
Publicado: (2021)

Successful fishing for nucleus pulposus progenitor cells of the intervertebral disc across species
por: Sakai, Daisuke, et al.
Publicado: (2018)

Ageing affects chondroitin sulfates and their synthetic enzymes in the intervertebral disc
por: Collin, Estelle C, et al.
Publicado: (2017)

Effect of different cryopreservation media on human nucleus pulposus cells' viability and trilineage potential
por: Croft, Andreas S., et al.
Publicado: (2021)

Roles of Silk Fibroin on Characteristics of Hyaluronic Acid/Silk Fibroin Hydrogels for Tissue Engineering of Nucleus Pulposus
por: Chung, Tze-Wen, et al.
Publicado: (2020)

Investigation of the Mitigation of DMSO-Induced Cytotoxicity by Hyaluronic Acid following Cryopreservation of Human Nucleus Pulposus Cells
por: Munesada, Daiki, et al.
Publicado: (2023)

Inducing Angiogenesis in the Nucleus Pulposus
por: Damle, Sheela R., et al.
Publicado: (2023)

Hypoxic stress enhances extension and branching of dorsal root ganglion neuronal outgrowth
por: Ma, Junxuan, et al.
Publicado: (2020)

A Hyaluronan and Platelet-Rich Plasma Hydrogel for Mesenchymal Stem Cell Delivery in the Intervertebral Disc: An Organ Culture Study
por: Russo, Fabrizio, et al.
Publicado: (2021)

Harmonization and standardization of nucleus pulposus cell extraction and culture methods
por: Basatvat, Shaghayegh, et al.
Publicado: (2023)

Collagen type II is downregulated in the degenerative nucleus pulposus and contributes to the degeneration and apoptosis of human nucleus pulposus cells
por: Lian, Chengjie, et al.
Publicado: (2017)

Expansion of FGFR3-positive nucleus pulposus cells plays important roles in postnatal nucleus pulposus growth and regeneration
por: Xu, Meng, et al.
Publicado: (2022)

Proinflammatory intervertebral disc cell and organ culture models induced by tumor necrosis factor alpha
por: Du, Jie, et al.
Publicado: (2020)

The enigma that is the nucleus pulposus cell: the search goes on
por: Erwin, W Mark
Publicado: (2010)

Bone Morphogenetic Proteins for Nucleus Pulposus Regeneration
por: Krouwels, Anita, et al.
Publicado: (2020)

Cell sources proposed for nucleus pulposus regeneration
por: Williams, Rebecca J., et al.
Publicado: (2021)

Alterations of bovine nucleus pulposus cells with aging
por: Molinos, Maria, et al.
Publicado: (2023)

Notochordal and nucleus pulposus marker expression is maintained by sub-populations of adult human nucleus pulposus cells through aging and degeneration
por: Richardson, Stephen M., et al.
Publicado: (2017)

“How to measure the outcome in the surgical treatment of vertebral compression fractures? A systematic literature review of highly cited level-I studies”
por: Häckel, Sonja, et al.
Publicado: (2021)

Nucleus Pulposus Cell Conditioned Medium Promotes Mesenchymal Stem Cell Differentiation into Nucleus Pulposus-Like Cells under Hypoxic Conditions
por: Sinkemani, Arjun, et al.
Publicado: (2020)

Effect of platelet-rich fibrin on epidural fibrosis and comparison to ADCON® Gel and hyaluronic acid
por: Demirel, Esra, et al.
Publicado: (2018)

Senescence of Nucleus Pulposus Chondrocytes in Human Intervertebral Discs
por: Kim, Ki-Won, et al.
Publicado: (2008)

Transcriptional profiling of the nucleus pulposus: say yes to notochord
por: Shapiro, Irving M, et al.
Publicado: (2010)

PHARMACOLOGIC TREATMENT OF HYPERALGESIA EXPERIMENTALLY INDUCED BY NUCLEUS PULPOSUS
por: de Souza Grava, André Luiz, et al.
Publicado: (2015)

Proteomic Signature of Nucleus Pulposus in Fetal Intervertebral Disc
por: Rajasekaran, Shanmuganathan, et al.
Publicado: (2020)

In vitro nucleus pulposus tissue model with physicochemical stresses
por: Takeoka, Yoshiki, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_i1efh8alam6snqdomn5ujjjurf