Cargando…

Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds

The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC)...

Descripción completa

Detalles Bibliográficos
Autores principales: García-Pérez, Pascual, Losada-Barreiro, Sonia, Gallego, Pedro P., Bravo-Díaz, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834148/
https://www.ncbi.nlm.nih.gov/pubmed/31635435
http://dx.doi.org/10.3390/ijms20205180
_version_ 1783466430022287360
author García-Pérez, Pascual
Losada-Barreiro, Sonia
Gallego, Pedro P.
Bravo-Díaz, Carlos
author_facet García-Pérez, Pascual
Losada-Barreiro, Sonia
Gallego, Pedro P.
Bravo-Díaz, Carlos
author_sort García-Pérez, Pascual
collection PubMed
description The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC) with cyclodextrins (CDs) as an alternative method for the production of bioactive compounds from Bryophyllum species. For this purpose, we analyzed the effects of methyl–β–cyclodextrin and 2–hydroxypropyl–β–cyclodextrin on cell culture growth and on the intra- and extracellular production of phenols and flavonoids. Results clearly show that CDs enhance the biosynthesis of polyphenols by PSCC favoring their accumulation outside the cells. CDs shift the homeostatic equilibrium by complexing extracellular phenolics, causing stress in cells that respond by increasing the production of intracellular phenolics. We also analyzed the radical scavenging activity of the culture medium extracts against 2,2–diphenyl–1–pycrilhydrazyl (DPPH) radical, which increased with respect to the control samples (no added CDs). Our results suggest that both the increase in the production of polyphenols and their radical scavenging activity are a consequence of their inclusion in the CD cavities. Overall, based on our findings, CDs can be employed as hosts for increasing the production of polyphenols from Bryophyllum species.
format Online
Article
Text
id pubmed-6834148
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68341482019-11-25 Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds García-Pérez, Pascual Losada-Barreiro, Sonia Gallego, Pedro P. Bravo-Díaz, Carlos Int J Mol Sci Article The rates of production of secondary metabolites obtained by employing conventional plant breeding may be low for practical purposes. Thus, innovative approaches for increasing their rates of production are being developed. Here, we propose the use of elicited plant suspension cultured cells (PSCC) with cyclodextrins (CDs) as an alternative method for the production of bioactive compounds from Bryophyllum species. For this purpose, we analyzed the effects of methyl–β–cyclodextrin and 2–hydroxypropyl–β–cyclodextrin on cell culture growth and on the intra- and extracellular production of phenols and flavonoids. Results clearly show that CDs enhance the biosynthesis of polyphenols by PSCC favoring their accumulation outside the cells. CDs shift the homeostatic equilibrium by complexing extracellular phenolics, causing stress in cells that respond by increasing the production of intracellular phenolics. We also analyzed the radical scavenging activity of the culture medium extracts against 2,2–diphenyl–1–pycrilhydrazyl (DPPH) radical, which increased with respect to the control samples (no added CDs). Our results suggest that both the increase in the production of polyphenols and their radical scavenging activity are a consequence of their inclusion in the CD cavities. Overall, based on our findings, CDs can be employed as hosts for increasing the production of polyphenols from Bryophyllum species. MDPI 2019-10-18 /pmc/articles/PMC6834148/ /pubmed/31635435 http://dx.doi.org/10.3390/ijms20205180 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
García-Pérez, Pascual
Losada-Barreiro, Sonia
Gallego, Pedro P.
Bravo-Díaz, Carlos
Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title_full Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title_fullStr Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title_full_unstemmed Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title_short Cyclodextrin-Elicited Bryophyllum Suspension Cultured Cells: Enhancement of the Production of Bioactive Compounds
title_sort cyclodextrin-elicited bryophyllum suspension cultured cells: enhancement of the production of bioactive compounds
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834148/
https://www.ncbi.nlm.nih.gov/pubmed/31635435
http://dx.doi.org/10.3390/ijms20205180
work_keys_str_mv AT garciaperezpascual cyclodextrinelicitedbryophyllumsuspensionculturedcellsenhancementoftheproductionofbioactivecompounds
AT losadabarreirosonia cyclodextrinelicitedbryophyllumsuspensionculturedcellsenhancementoftheproductionofbioactivecompounds
AT gallegopedrop cyclodextrinelicitedbryophyllumsuspensionculturedcellsenhancementoftheproductionofbioactivecompounds
AT bravodiazcarlos cyclodextrinelicitedbryophyllumsuspensionculturedcellsenhancementoftheproductionofbioactivecompounds