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Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus

Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectivel...

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Autores principales: Shi, Xiao-Bin, Wang, Xue-Zhong, Zhang, De-Yong, Zhang, Zhan-Hong, Zhang, Zhuo, Cheng, Ju’E, Zheng, Li-Min, Zhou, Xu-Guo, Tan, Xin-Qiu, Liu, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834158/
https://www.ncbi.nlm.nih.gov/pubmed/31600869
http://dx.doi.org/10.3390/ijms20204969
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author Shi, Xiao-Bin
Wang, Xue-Zhong
Zhang, De-Yong
Zhang, Zhan-Hong
Zhang, Zhuo
Cheng, Ju’E
Zheng, Li-Min
Zhou, Xu-Guo
Tan, Xin-Qiu
Liu, Yong
author_facet Shi, Xiao-Bin
Wang, Xue-Zhong
Zhang, De-Yong
Zhang, Zhan-Hong
Zhang, Zhuo
Cheng, Ju’E
Zheng, Li-Min
Zhou, Xu-Guo
Tan, Xin-Qiu
Liu, Yong
author_sort Shi, Xiao-Bin
collection PubMed
description Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectively control B. tabaci and whitefly-borne virus diseases. Despite current research efforts, no report has been published on the role of odorant-binding proteins (OBPs) in insect preference under the influence of plant virus. Our previous research showed that viruliferous B. tabaci preferred healthy plants at 48 h after virus acquisition. In this study, we determined the effect of OBPs on the host preference interactions of ToCV and whiteflies. Our results show that with the increase in acquisition time, the OBP gene expressions changed differently, and the OBP3 gene expression showed a trend of first rising and then falling, and reached the maximum at 48 h. These results indicate that OBP3 may participate in the host preference of viruliferous whiteflies to healthy plants. When the expression of the OBP3 gene was knocked down by an RNA interference (RNAi) technique, viruliferous Mediterranean (MED) showed no preference and the ToCV transmission rate was reduced by 83.3%. We conclude that OBP3 is involved in the detection of plant volatiles by viruliferous MED. Our results provide a theoretical basis and technical support for clarifying the transmission mechanism of ToCV by B. tabaci and could provide new avenues for controlling this plant virus and its vectors.
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spelling pubmed-68341582019-11-25 Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus Shi, Xiao-Bin Wang, Xue-Zhong Zhang, De-Yong Zhang, Zhan-Hong Zhang, Zhuo Cheng, Ju’E Zheng, Li-Min Zhou, Xu-Guo Tan, Xin-Qiu Liu, Yong Int J Mol Sci Article Tomato chlorosis virus (ToCV) is widespread, seriously impacting tomato production throughout the world. ToCV is semi-persistently transmitted by Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae). Currently, insect olfaction is being studied to develop novel pest control technologies to effectively control B. tabaci and whitefly-borne virus diseases. Despite current research efforts, no report has been published on the role of odorant-binding proteins (OBPs) in insect preference under the influence of plant virus. Our previous research showed that viruliferous B. tabaci preferred healthy plants at 48 h after virus acquisition. In this study, we determined the effect of OBPs on the host preference interactions of ToCV and whiteflies. Our results show that with the increase in acquisition time, the OBP gene expressions changed differently, and the OBP3 gene expression showed a trend of first rising and then falling, and reached the maximum at 48 h. These results indicate that OBP3 may participate in the host preference of viruliferous whiteflies to healthy plants. When the expression of the OBP3 gene was knocked down by an RNA interference (RNAi) technique, viruliferous Mediterranean (MED) showed no preference and the ToCV transmission rate was reduced by 83.3%. We conclude that OBP3 is involved in the detection of plant volatiles by viruliferous MED. Our results provide a theoretical basis and technical support for clarifying the transmission mechanism of ToCV by B. tabaci and could provide new avenues for controlling this plant virus and its vectors. MDPI 2019-10-09 /pmc/articles/PMC6834158/ /pubmed/31600869 http://dx.doi.org/10.3390/ijms20204969 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shi, Xiao-Bin
Wang, Xue-Zhong
Zhang, De-Yong
Zhang, Zhan-Hong
Zhang, Zhuo
Cheng, Ju’E
Zheng, Li-Min
Zhou, Xu-Guo
Tan, Xin-Qiu
Liu, Yong
Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title_full Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title_fullStr Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title_full_unstemmed Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title_short Silencing of Odorant-Binding Protein Gene OBP3 Using RNA Interference Reduced Virus Transmission of Tomato Chlorosis Virus
title_sort silencing of odorant-binding protein gene obp3 using rna interference reduced virus transmission of tomato chlorosis virus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834158/
https://www.ncbi.nlm.nih.gov/pubmed/31600869
http://dx.doi.org/10.3390/ijms20204969
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