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SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy
A homozygous mutation of human tyrosyl-DNA phosphodiesterase 1 (TDP1) causes the neurodegenerative syndrome, spinocerebellar ataxia with axonal neuropathy (SCAN1). TDP1 hydrolyzes the phosphodiester bond between DNA 3′-end and a tyrosyl moiety within trapped topoisomerase I (Top1)-DNA covalent compl...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Association for the Advancement of Science
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834389/ https://www.ncbi.nlm.nih.gov/pubmed/31723605 http://dx.doi.org/10.1126/sciadv.aax9778 |
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author | Ghosh, Arijit Bhattacharjee, Sangheeta Chowdhuri, Srijita Paul Mallick, Abhik Rehman, Ishita Basu, Sudipta Das, Benu Brata |
author_facet | Ghosh, Arijit Bhattacharjee, Sangheeta Chowdhuri, Srijita Paul Mallick, Abhik Rehman, Ishita Basu, Sudipta Das, Benu Brata |
author_sort | Ghosh, Arijit |
collection | PubMed |
description | A homozygous mutation of human tyrosyl-DNA phosphodiesterase 1 (TDP1) causes the neurodegenerative syndrome, spinocerebellar ataxia with axonal neuropathy (SCAN1). TDP1 hydrolyzes the phosphodiester bond between DNA 3′-end and a tyrosyl moiety within trapped topoisomerase I (Top1)-DNA covalent complexes (Top1cc). TDP1 is critical for mitochondrial DNA (mtDNA) repair; however, the role of mitochondria remains largely unknown for the etiology of SCAN1. We demonstrate that mitochondria in cells expressing SCAN1-TDP1 (TDP1(H493R)) are selectively trapped on mtDNA in the regulatory non-coding region and promoter sequences. Trapped TDP1(H493R)-mtDNA complexes were markedly increased in the presence of the Top1 poison (mito-SN38) when targeted selectively into mitochondria in nanoparticles. TDP1(H493R)-trapping accumulates mtDNA damage and triggers Drp1-mediated mitochondrial fission, which blocks mitobiogenesis. TDP1(H493R) prompts PTEN-induced kinase 1–dependent mitophagy to eliminate dysfunctional mitochondria. SCAN1-TDP1 in mitochondria creates a pathological state that allows neurons to turn on mitophagy to rescue fit mitochondria as a mechanism of survival. |
format | Online Article Text |
id | pubmed-6834389 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | American Association for the Advancement of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-68343892019-11-13 SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy Ghosh, Arijit Bhattacharjee, Sangheeta Chowdhuri, Srijita Paul Mallick, Abhik Rehman, Ishita Basu, Sudipta Das, Benu Brata Sci Adv Research Articles A homozygous mutation of human tyrosyl-DNA phosphodiesterase 1 (TDP1) causes the neurodegenerative syndrome, spinocerebellar ataxia with axonal neuropathy (SCAN1). TDP1 hydrolyzes the phosphodiester bond between DNA 3′-end and a tyrosyl moiety within trapped topoisomerase I (Top1)-DNA covalent complexes (Top1cc). TDP1 is critical for mitochondrial DNA (mtDNA) repair; however, the role of mitochondria remains largely unknown for the etiology of SCAN1. We demonstrate that mitochondria in cells expressing SCAN1-TDP1 (TDP1(H493R)) are selectively trapped on mtDNA in the regulatory non-coding region and promoter sequences. Trapped TDP1(H493R)-mtDNA complexes were markedly increased in the presence of the Top1 poison (mito-SN38) when targeted selectively into mitochondria in nanoparticles. TDP1(H493R)-trapping accumulates mtDNA damage and triggers Drp1-mediated mitochondrial fission, which blocks mitobiogenesis. TDP1(H493R) prompts PTEN-induced kinase 1–dependent mitophagy to eliminate dysfunctional mitochondria. SCAN1-TDP1 in mitochondria creates a pathological state that allows neurons to turn on mitophagy to rescue fit mitochondria as a mechanism of survival. American Association for the Advancement of Science 2019-11-06 /pmc/articles/PMC6834389/ /pubmed/31723605 http://dx.doi.org/10.1126/sciadv.aax9778 Text en Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (http://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited. |
spellingShingle | Research Articles Ghosh, Arijit Bhattacharjee, Sangheeta Chowdhuri, Srijita Paul Mallick, Abhik Rehman, Ishita Basu, Sudipta Das, Benu Brata SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title | SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title_full | SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title_fullStr | SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title_full_unstemmed | SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title_short | SCAN1-TDP1 trapping on mitochondrial DNA promotes mitochondrial dysfunction and mitophagy |
title_sort | scan1-tdp1 trapping on mitochondrial dna promotes mitochondrial dysfunction and mitophagy |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834389/ https://www.ncbi.nlm.nih.gov/pubmed/31723605 http://dx.doi.org/10.1126/sciadv.aax9778 |
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