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Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology

We present an approach for fabrication of reproducible, chemically and mechanically robust functionalized layers based on MgF(2) thin films on thin glass substrates. These show great advantages for use in super-resolution microscopy as well as for multi-electrode-array fabrication and are especially...

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Autores principales: Schmidl, L., Schmidl, G., Gawlik, A., Dellith, J., Hübner, U., Tympel, V., Schmidl, F., Plentz, J., Geis, C., Haselmann, H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834630/
https://www.ncbi.nlm.nih.gov/pubmed/31695073
http://dx.doi.org/10.1038/s41598-019-52397-x
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author Schmidl, L.
Schmidl, G.
Gawlik, A.
Dellith, J.
Hübner, U.
Tympel, V.
Schmidl, F.
Plentz, J.
Geis, C.
Haselmann, H.
author_facet Schmidl, L.
Schmidl, G.
Gawlik, A.
Dellith, J.
Hübner, U.
Tympel, V.
Schmidl, F.
Plentz, J.
Geis, C.
Haselmann, H.
author_sort Schmidl, L.
collection PubMed
description We present an approach for fabrication of reproducible, chemically and mechanically robust functionalized layers based on MgF(2) thin films on thin glass substrates. These show great advantages for use in super-resolution microscopy as well as for multi-electrode-array fabrication and are especially suited for combination of these techniques. The transparency of the coated substrates with the low refractive index material is adjustable by the layer thickness and can be increased above 92%. Due to the hydrophobic and lipophilic properties of the thin crystalline MgF(2) layers, the temporal stable adhesion needed for fixation of thin tissue, e.g. cryogenic brain slices is given. This has been tested using localization-based super-resolution microscopy with currently highest spatial resolution in light microscopy. We demonstrated that direct stochastic optical reconstruction microscopy revealed in reliable imaging of structures of central synapses by use of double immunostaining of post- (homer1 and GluA2) and presynaptic (bassoon) marker structure in a 10 µm brain slice without additional fixing of the slices. Due to the proven additional electrical insulating effect of MgF(2) layers, surfaces of multi-electrode-arrays were coated with this material and tested by voltage-current-measurements. MgF(2) coated multi-electrode-arrays can be used as a functionalized microscope cover slip for combination with live-cell super-resolution microscopy.
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spelling pubmed-68346302019-11-14 Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology Schmidl, L. Schmidl, G. Gawlik, A. Dellith, J. Hübner, U. Tympel, V. Schmidl, F. Plentz, J. Geis, C. Haselmann, H. Sci Rep Article We present an approach for fabrication of reproducible, chemically and mechanically robust functionalized layers based on MgF(2) thin films on thin glass substrates. These show great advantages for use in super-resolution microscopy as well as for multi-electrode-array fabrication and are especially suited for combination of these techniques. The transparency of the coated substrates with the low refractive index material is adjustable by the layer thickness and can be increased above 92%. Due to the hydrophobic and lipophilic properties of the thin crystalline MgF(2) layers, the temporal stable adhesion needed for fixation of thin tissue, e.g. cryogenic brain slices is given. This has been tested using localization-based super-resolution microscopy with currently highest spatial resolution in light microscopy. We demonstrated that direct stochastic optical reconstruction microscopy revealed in reliable imaging of structures of central synapses by use of double immunostaining of post- (homer1 and GluA2) and presynaptic (bassoon) marker structure in a 10 µm brain slice without additional fixing of the slices. Due to the proven additional electrical insulating effect of MgF(2) layers, surfaces of multi-electrode-arrays were coated with this material and tested by voltage-current-measurements. MgF(2) coated multi-electrode-arrays can be used as a functionalized microscope cover slip for combination with live-cell super-resolution microscopy. Nature Publishing Group UK 2019-11-06 /pmc/articles/PMC6834630/ /pubmed/31695073 http://dx.doi.org/10.1038/s41598-019-52397-x Text en © The Author(s) 2019 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Schmidl, L.
Schmidl, G.
Gawlik, A.
Dellith, J.
Hübner, U.
Tympel, V.
Schmidl, F.
Plentz, J.
Geis, C.
Haselmann, H.
Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title_full Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title_fullStr Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title_full_unstemmed Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title_short Combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
title_sort combining super-resolution microscopy with neuronal network recording using magnesium fluoride thin films as cover layer for multi-electrode array technology
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834630/
https://www.ncbi.nlm.nih.gov/pubmed/31695073
http://dx.doi.org/10.1038/s41598-019-52397-x
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