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In vivo CRISPR screening in CD8 T cells with AAV-Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma

Identification of T cell targets to improve immunotherapies is of prime interest. To facilitate large-scale CRISPR screens directly in T cells in vivo, here, we developed a hybrid genetic screening system with adeno associated virus (AAV) and the Sleeping Beauty (SB) transposon, where the transposon...

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Detalles Bibliográficos
Autores principales:	Ye, Lupeng, Park, Jonathan J., Dong, Matthew B., Yang, Quanjun, Chow, Ryan D., Peng, Lei, Du, Yaying, Guo, Jianjian, Dai, Xiaoyun, Wang, Guangchuan, Errami, Youssef, Chen, Sidi
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	2019
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6834896/ https://www.ncbi.nlm.nih.gov/pubmed/31548728 http://dx.doi.org/10.1038/s41587-019-0246-4

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