Training session intensity affects plasma redox status in amateur rhythmic gymnasts

PURPOSE: The aim of this study was to examine systemic responses of oxidant/antioxidant status following 2 training sessions of different intensity in amateur rhythmic gymnasts. METHODS: Before the experimental training, 10 female gymnasts performed a gradually increased exercise test to assess maximal heart rate, maximal oxygen consumption, and anaerobic threshold. They executed 2 intermittent training sessions separated by 48 h of recovery (48 h-post R): the first was performed at low-moderate intensity (LMI) and the second at high intensity (HI). Blood samples were collected immediately pre- and post-training and 48 h-post R. Hydroperoxide level (OxL) and total antioxidant capacity (TAC) were photometrically measured. RESULTS: OxL was significantly higher in post-training and 48 h-post R following HI than the same conditions after an LMI session (HI vs. LMI post-training: 381.10 ± 46.17 (mean ± SD) vs. 344.18 ± 27.94 Units Carratelli (U.CARR); 48 h-post R: 412.21 ± 26.61 vs. 373.80 ± 36.08 U.CARR). There was no change in TAC between the 2 training sessions investigated. In LMI training, OxL significantly decreased in post-training and increased to reach the baseline at 48 h-post R, whereas TAC increased only at 48 h-post R. In HI training, OxL significantly increased to reach a high oxidative stress 48 h-post R, whereas TAC was lower in post-training than pre-training. CONCLUSION: The pattern of OxL and TAC levels implies different regulation mechanisms by HI and LMI training sessions. High oxidative stress induced by an HI protocol might be associated with both insufficient TAC and recovery time at 48 h necessary to restore redox balance.