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Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck

Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm...

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Autores principales: Watermann, Anna, Gieringer, Rita, Bauer, Anna-Maria, Kurch, Sven, Kiesslich, Ralf, Tremel, Wolfgang, Gosepath, Jan, Brieger, Juergen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835239/
https://www.ncbi.nlm.nih.gov/pubmed/31561451
http://dx.doi.org/10.3390/nano9101378
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author Watermann, Anna
Gieringer, Rita
Bauer, Anna-Maria
Kurch, Sven
Kiesslich, Ralf
Tremel, Wolfgang
Gosepath, Jan
Brieger, Juergen
author_facet Watermann, Anna
Gieringer, Rita
Bauer, Anna-Maria
Kurch, Sven
Kiesslich, Ralf
Tremel, Wolfgang
Gosepath, Jan
Brieger, Juergen
author_sort Watermann, Anna
collection PubMed
description Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection.
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spelling pubmed-68352392019-11-25 Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck Watermann, Anna Gieringer, Rita Bauer, Anna-Maria Kurch, Sven Kiesslich, Ralf Tremel, Wolfgang Gosepath, Jan Brieger, Juergen Nanomaterials (Basel) Article Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection. MDPI 2019-09-26 /pmc/articles/PMC6835239/ /pubmed/31561451 http://dx.doi.org/10.3390/nano9101378 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Watermann, Anna
Gieringer, Rita
Bauer, Anna-Maria
Kurch, Sven
Kiesslich, Ralf
Tremel, Wolfgang
Gosepath, Jan
Brieger, Juergen
Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title_full Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title_fullStr Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title_full_unstemmed Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title_short Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
title_sort fluorescein- and egfr-antibody conjugated silica nanoparticles for enhancement of real-time tumor border definition using confocal laser endomicroscopy in squamous cell carcinoma of the head and neck
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835239/
https://www.ncbi.nlm.nih.gov/pubmed/31561451
http://dx.doi.org/10.3390/nano9101378
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