Cargando…
Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck
Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835239/ https://www.ncbi.nlm.nih.gov/pubmed/31561451 http://dx.doi.org/10.3390/nano9101378 |
_version_ | 1783466625628897280 |
---|---|
author | Watermann, Anna Gieringer, Rita Bauer, Anna-Maria Kurch, Sven Kiesslich, Ralf Tremel, Wolfgang Gosepath, Jan Brieger, Juergen |
author_facet | Watermann, Anna Gieringer, Rita Bauer, Anna-Maria Kurch, Sven Kiesslich, Ralf Tremel, Wolfgang Gosepath, Jan Brieger, Juergen |
author_sort | Watermann, Anna |
collection | PubMed |
description | Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection. |
format | Online Article Text |
id | pubmed-6835239 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68352392019-11-25 Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck Watermann, Anna Gieringer, Rita Bauer, Anna-Maria Kurch, Sven Kiesslich, Ralf Tremel, Wolfgang Gosepath, Jan Brieger, Juergen Nanomaterials (Basel) Article Intraoperative definition of tumor free resection margins in head and neck cancer is challenging. In the current proof-of-principle study we evaluated a novel silica nanoparticle-based agent for its potential use as contrast enhancer. We synthesized silica nanoparticles with an average size of 45 nm and modified these particles with the fluorescence stain fluorescein isocyanate (FITC) for particle detection and with epidermal growth factor receptor (EGFR)-targeting antibodies for enhanced tumor specificity. The nanoparticles exhibited good biocompatibility and could be detected in vitro and in vivo by confocal laser scanning microscopy. Additionally, we show in an ex vivo setting that these modified nanoparticles specifically bind to tumor samples and could be detected using a handheld confocal fluorescence endomicroscope. From a clinical point of view, we believe that this method could be used for tumor border contrast enhancement and for better intraoperative definition of R-0 tumor resection. MDPI 2019-09-26 /pmc/articles/PMC6835239/ /pubmed/31561451 http://dx.doi.org/10.3390/nano9101378 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Watermann, Anna Gieringer, Rita Bauer, Anna-Maria Kurch, Sven Kiesslich, Ralf Tremel, Wolfgang Gosepath, Jan Brieger, Juergen Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title_full | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title_fullStr | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title_full_unstemmed | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title_short | Fluorescein- and EGFR-Antibody Conjugated Silica Nanoparticles for Enhancement of Real-time Tumor Border Definition Using Confocal Laser Endomicroscopy in Squamous Cell Carcinoma of the Head and Neck |
title_sort | fluorescein- and egfr-antibody conjugated silica nanoparticles for enhancement of real-time tumor border definition using confocal laser endomicroscopy in squamous cell carcinoma of the head and neck |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835239/ https://www.ncbi.nlm.nih.gov/pubmed/31561451 http://dx.doi.org/10.3390/nano9101378 |
work_keys_str_mv | AT watermannanna fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT gieringerrita fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT bauerannamaria fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT kurchsven fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT kiesslichralf fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT tremelwolfgang fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT gosepathjan fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck AT briegerjuergen fluoresceinandegfrantibodyconjugatedsilicananoparticlesforenhancementofrealtimetumorborderdefinitionusingconfocallaserendomicroscopyinsquamouscellcarcinomaoftheheadandneck |