Cargando…

Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians

Lactovegetarians (n = 35) with low vitamin B12 (B12) status were intervened for eight weeks capsules containing cyano-B12 (CN-B12), (2 × 2.8 µg/day), or equivalent doses of endogenous B12 (mainly hydroxo-B12 (HO-B12)) in whey powder. Blood samples were examined at baseline, every second week during...

Descripción completa

Detalles Bibliográficos
Autores principales: Naik, Sadanand, Mahalle, Namita, Greibe, Eva, Ostenfeld, Marie S., Heegaard, Christian W., Nexo, Ebba, Fedosov, Sergey N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835307/
https://www.ncbi.nlm.nih.gov/pubmed/31590426
http://dx.doi.org/10.3390/nu11102382
_version_ 1783466641409966080
author Naik, Sadanand
Mahalle, Namita
Greibe, Eva
Ostenfeld, Marie S.
Heegaard, Christian W.
Nexo, Ebba
Fedosov, Sergey N.
author_facet Naik, Sadanand
Mahalle, Namita
Greibe, Eva
Ostenfeld, Marie S.
Heegaard, Christian W.
Nexo, Ebba
Fedosov, Sergey N.
author_sort Naik, Sadanand
collection PubMed
description Lactovegetarians (n = 35) with low vitamin B12 (B12) status were intervened for eight weeks capsules containing cyano-B12 (CN-B12), (2 × 2.8 µg/day), or equivalent doses of endogenous B12 (mainly hydroxo-B12 (HO-B12)) in whey powder. Blood samples were examined at baseline, every second week during the intervention, and two weeks post-intervention. The groups did not differ at baseline in [global median (min/max)] plasma B12 [112(61/185)] pmol/L, holotranscobalamin [20(4/99)] pmol/L, folate [13(11/16)], the metabolites total homocysteine [18(9/52)] µmol/L and methylmalonic acid [0.90(0.28/2.5)] µmol/L, and the combined indicator of B12 status (4cB12) [−1.7(−3.0/−0.33)]. Both supplements caused significant effects, though none of the biomarkers returned to normal values. Total plasma B12 showed a higher increase in the capsule group compared to the whey powder group (p = 0.02). However, the increase of plasma holotranscobalamin (p = 0.06) and the lowering of the metabolites (p > 0.07) were alike in both groups. Thereby, the high total plasma B12 in the capsule group was not mirrored in enhanced B12 metabolism, possibly because the B12 surplus was mainly accumulated on an “inert” carrier haptocorrin, considered to be of marginal importance for tissue delivery of B12. In conclusion, we demonstrate that administration of whey powder (HO-B12) or capsules (CN-B12) equivalent to 5.6 µg of B12 daily for eight weeks similarly improves B12 status but does not normalize it. We document that the results for plasma B12 should be interpreted with caution following administration of CN-B12, since the change is disproportionately high compared to the responses of complementary biomarkers.
format Online
Article
Text
id pubmed-6835307
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68353072019-11-25 Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians Naik, Sadanand Mahalle, Namita Greibe, Eva Ostenfeld, Marie S. Heegaard, Christian W. Nexo, Ebba Fedosov, Sergey N. Nutrients Article Lactovegetarians (n = 35) with low vitamin B12 (B12) status were intervened for eight weeks capsules containing cyano-B12 (CN-B12), (2 × 2.8 µg/day), or equivalent doses of endogenous B12 (mainly hydroxo-B12 (HO-B12)) in whey powder. Blood samples were examined at baseline, every second week during the intervention, and two weeks post-intervention. The groups did not differ at baseline in [global median (min/max)] plasma B12 [112(61/185)] pmol/L, holotranscobalamin [20(4/99)] pmol/L, folate [13(11/16)], the metabolites total homocysteine [18(9/52)] µmol/L and methylmalonic acid [0.90(0.28/2.5)] µmol/L, and the combined indicator of B12 status (4cB12) [−1.7(−3.0/−0.33)]. Both supplements caused significant effects, though none of the biomarkers returned to normal values. Total plasma B12 showed a higher increase in the capsule group compared to the whey powder group (p = 0.02). However, the increase of plasma holotranscobalamin (p = 0.06) and the lowering of the metabolites (p > 0.07) were alike in both groups. Thereby, the high total plasma B12 in the capsule group was not mirrored in enhanced B12 metabolism, possibly because the B12 surplus was mainly accumulated on an “inert” carrier haptocorrin, considered to be of marginal importance for tissue delivery of B12. In conclusion, we demonstrate that administration of whey powder (HO-B12) or capsules (CN-B12) equivalent to 5.6 µg of B12 daily for eight weeks similarly improves B12 status but does not normalize it. We document that the results for plasma B12 should be interpreted with caution following administration of CN-B12, since the change is disproportionately high compared to the responses of complementary biomarkers. MDPI 2019-10-06 /pmc/articles/PMC6835307/ /pubmed/31590426 http://dx.doi.org/10.3390/nu11102382 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Naik, Sadanand
Mahalle, Namita
Greibe, Eva
Ostenfeld, Marie S.
Heegaard, Christian W.
Nexo, Ebba
Fedosov, Sergey N.
Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title_full Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title_fullStr Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title_full_unstemmed Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title_short Cyano-B12 or Whey Powder with Endogenous Hydroxo-B12 for Supplementation in B12 Deficient Lactovegetarians
title_sort cyano-b12 or whey powder with endogenous hydroxo-b12 for supplementation in b12 deficient lactovegetarians
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835307/
https://www.ncbi.nlm.nih.gov/pubmed/31590426
http://dx.doi.org/10.3390/nu11102382
work_keys_str_mv AT naiksadanand cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT mahallenamita cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT greibeeva cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT ostenfeldmaries cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT heegaardchristianw cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT nexoebba cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians
AT fedosovsergeyn cyanob12orwheypowderwithendogenoushydroxob12forsupplementationinb12deficientlactovegetarians