Cargando…

Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression

β-amyloid formation in the brain is one of the characteristics of Alzheimer’s disease. Exposure to this peptide may result in reentry into the cell cycle leading to cell death. The phytoestrogen equol has similar biological effects as estrogen without the side effects. This study investigated the po...

Descripción completa

Detalles Bibliográficos
Autores principales: Tsai, Meng-Chao, Lin, Shyh-Hsiang, Hidayah, Kiswatul, Lin, Ching-I
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835339/
https://www.ncbi.nlm.nih.gov/pubmed/31623342
http://dx.doi.org/10.3390/nu11102356
_version_ 1783466649091833856
author Tsai, Meng-Chao
Lin, Shyh-Hsiang
Hidayah, Kiswatul
Lin, Ching-I
author_facet Tsai, Meng-Chao
Lin, Shyh-Hsiang
Hidayah, Kiswatul
Lin, Ching-I
author_sort Tsai, Meng-Chao
collection PubMed
description β-amyloid formation in the brain is one of the characteristics of Alzheimer’s disease. Exposure to this peptide may result in reentry into the cell cycle leading to cell death. The phytoestrogen equol has similar biological effects as estrogen without the side effects. This study investigated the possible mechanism of the neuron cell-protecting effect of equol during treatment with Aβ. SH-SY5Y neuroblastoma cells were treated with either 1 μM S-equol or 10 nM 17β-estradiol for 24 h prior to 1 μM Aβ (25–35) exposure. After 24 h exposure to Aβ (25–35), a significant reduction in cell survival and a reentry into the cell cycle process accompanied by increased levels of cyclin D1 were observed. The expressions of estrogen receptor alpha (ERα) and its coactivator, steroid receptor coactivator-1 (SRC-1), were also significantly downregulated by Aβ (25–35) in parallel with activated extracellular signal-regulated kinase (ERK)1/2. However, pretreatment of cells with S-equol or 17β-estradiol reversed these effects. Treatment with the ER antagonist, ICI-182,780 (1 μM), completely blocked the effects of S-equol and 17β-estradiol on cell viability, ERα, and ERK1/2 after Aβ (25–35) exposure. These data suggest that S-equol possesses a neuroprotective potential as it effectively antagonizes Aβ (25–35)-induced cell cytotoxicity and prevents cell cycle reentry in SH-SY5Y cells. The mechanism underlying S-equol neuroprotection might involve ERα-mediated pathways.
format Online
Article
Text
id pubmed-6835339
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68353392019-11-25 Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression Tsai, Meng-Chao Lin, Shyh-Hsiang Hidayah, Kiswatul Lin, Ching-I Nutrients Article β-amyloid formation in the brain is one of the characteristics of Alzheimer’s disease. Exposure to this peptide may result in reentry into the cell cycle leading to cell death. The phytoestrogen equol has similar biological effects as estrogen without the side effects. This study investigated the possible mechanism of the neuron cell-protecting effect of equol during treatment with Aβ. SH-SY5Y neuroblastoma cells were treated with either 1 μM S-equol or 10 nM 17β-estradiol for 24 h prior to 1 μM Aβ (25–35) exposure. After 24 h exposure to Aβ (25–35), a significant reduction in cell survival and a reentry into the cell cycle process accompanied by increased levels of cyclin D1 were observed. The expressions of estrogen receptor alpha (ERα) and its coactivator, steroid receptor coactivator-1 (SRC-1), were also significantly downregulated by Aβ (25–35) in parallel with activated extracellular signal-regulated kinase (ERK)1/2. However, pretreatment of cells with S-equol or 17β-estradiol reversed these effects. Treatment with the ER antagonist, ICI-182,780 (1 μM), completely blocked the effects of S-equol and 17β-estradiol on cell viability, ERα, and ERK1/2 after Aβ (25–35) exposure. These data suggest that S-equol possesses a neuroprotective potential as it effectively antagonizes Aβ (25–35)-induced cell cytotoxicity and prevents cell cycle reentry in SH-SY5Y cells. The mechanism underlying S-equol neuroprotection might involve ERα-mediated pathways. MDPI 2019-10-03 /pmc/articles/PMC6835339/ /pubmed/31623342 http://dx.doi.org/10.3390/nu11102356 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Meng-Chao
Lin, Shyh-Hsiang
Hidayah, Kiswatul
Lin, Ching-I
Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title_full Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title_fullStr Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title_full_unstemmed Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title_short Equol Pretreatment Protection of SH-SY5Y Cells against Aβ (25–35)-Induced Cytotoxicity and Cell-Cycle Reentry via Sustaining Estrogen Receptor Alpha Expression
title_sort equol pretreatment protection of sh-sy5y cells against aβ (25–35)-induced cytotoxicity and cell-cycle reentry via sustaining estrogen receptor alpha expression
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835339/
https://www.ncbi.nlm.nih.gov/pubmed/31623342
http://dx.doi.org/10.3390/nu11102356
work_keys_str_mv AT tsaimengchao equolpretreatmentprotectionofshsy5ycellsagainstab2535inducedcytotoxicityandcellcyclereentryviasustainingestrogenreceptoralphaexpression
AT linshyhhsiang equolpretreatmentprotectionofshsy5ycellsagainstab2535inducedcytotoxicityandcellcyclereentryviasustainingestrogenreceptoralphaexpression
AT hidayahkiswatul equolpretreatmentprotectionofshsy5ycellsagainstab2535inducedcytotoxicityandcellcyclereentryviasustainingestrogenreceptoralphaexpression
AT linchingi equolpretreatmentprotectionofshsy5ycellsagainstab2535inducedcytotoxicityandcellcyclereentryviasustainingestrogenreceptoralphaexpression