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Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection

Nanoparticles derived from the elongated flexuous capsids of Turnip mosaic virus (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particle...

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Autores principales: Yuste-Calvo, Carmen, López-Santalla, Mercedes, Zurita, Lucía, Cruz-Fernández, César F., Sánchez, Flora, Garín, Marina I., Ponz, Fernando
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835482/
https://www.ncbi.nlm.nih.gov/pubmed/31658770
http://dx.doi.org/10.3390/nano9101438
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author Yuste-Calvo, Carmen
López-Santalla, Mercedes
Zurita, Lucía
Cruz-Fernández, César F.
Sánchez, Flora
Garín, Marina I.
Ponz, Fernando
author_facet Yuste-Calvo, Carmen
López-Santalla, Mercedes
Zurita, Lucía
Cruz-Fernández, César F.
Sánchez, Flora
Garín, Marina I.
Ponz, Fernando
author_sort Yuste-Calvo, Carmen
collection PubMed
description Nanoparticles derived from the elongated flexuous capsids of Turnip mosaic virus (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs.
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spelling pubmed-68354822019-11-25 Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection Yuste-Calvo, Carmen López-Santalla, Mercedes Zurita, Lucía Cruz-Fernández, César F. Sánchez, Flora Garín, Marina I. Ponz, Fernando Nanomaterials (Basel) Article Nanoparticles derived from the elongated flexuous capsids of Turnip mosaic virus (TuMV) have been shown to be efficient tools for antibody sensing with a very high sensitivity if adequately functionalized with the corresponding epitopes. Taking advantage of this possibility, TuMV virus-like particles (VLPs) have been genetically derivatized with a peptide from the chaperonin Hsp60, a protein described to be involved in inflammation processes and autoimmune diseases. Antibodies against the peptide have been previously shown to have a diagnostic value in at least one autoimmune disease, multiple sclerosis. The functionalized Hsp60-VLPs showed their significant increase in sensing potency when compared to monoclonal antibody detection of the peptide in a conventional immunoassay. Additionally, the developed Hsp60-VLPs allowed the detection of autoantibodies against the Hsp60 peptide in an in vivo mouse model of dextran sodium sulfate (DSS)-induced colitis. The detection of minute amounts of the autoantibodies allowed us to perform the analysis of their evolution during the progression of the disease. The anti-Hsp60 autoantibody levels in the sera of the inflamed mice went down during the induction phase of the disease. Increased levels of the anti-HSP60 autoantibodies were detected during the resolution phase of the disease. An extension of a previously proposed model for the involvement of Hsp60 in inflammatory processes is considered, incorporating a role for Hsp60 autoantibodies. This, and related models, can now be experimentally tested thanks to the autoantibody detection hypersensitivity provided by the functionalized VLPs. MDPI 2019-10-10 /pmc/articles/PMC6835482/ /pubmed/31658770 http://dx.doi.org/10.3390/nano9101438 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yuste-Calvo, Carmen
López-Santalla, Mercedes
Zurita, Lucía
Cruz-Fernández, César F.
Sánchez, Flora
Garín, Marina I.
Ponz, Fernando
Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title_full Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title_fullStr Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title_full_unstemmed Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title_short Elongated Flexuous Plant Virus-Derived Nanoparticles Functionalized for Autoantibody Detection
title_sort elongated flexuous plant virus-derived nanoparticles functionalized for autoantibody detection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835482/
https://www.ncbi.nlm.nih.gov/pubmed/31658770
http://dx.doi.org/10.3390/nano9101438
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