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Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease

Our aim was to investigate whether hot water extract (CLW) of Curcuma longa L. could prevent non-alcoholic fatty liver disease (NAFLD). HepG2 cells were treated with free fatty acid (FFA) mixture (oleic acid: palmitic acid, 2:1) for 24 h to stimulate in vitro fatty liver. In addition, C57BL/6 mice w...

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Autores principales: Mun, Jeongeun, Kim, Shintae, Yoon, Ho-Geun, You, Yanghee, Kim, Ok-Kyung, Choi, Kyung-Chul, Lee, Yoo-Hyun, Lee, Jeongmin, Park, Jeongjin, Jun, Woojin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835554/
https://www.ncbi.nlm.nih.gov/pubmed/31640183
http://dx.doi.org/10.3390/nu11102536
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author Mun, Jeongeun
Kim, Shintae
Yoon, Ho-Geun
You, Yanghee
Kim, Ok-Kyung
Choi, Kyung-Chul
Lee, Yoo-Hyun
Lee, Jeongmin
Park, Jeongjin
Jun, Woojin
author_facet Mun, Jeongeun
Kim, Shintae
Yoon, Ho-Geun
You, Yanghee
Kim, Ok-Kyung
Choi, Kyung-Chul
Lee, Yoo-Hyun
Lee, Jeongmin
Park, Jeongjin
Jun, Woojin
author_sort Mun, Jeongeun
collection PubMed
description Our aim was to investigate whether hot water extract (CLW) of Curcuma longa L. could prevent non-alcoholic fatty liver disease (NAFLD). HepG2 cells were treated with free fatty acid (FFA) mixture (oleic acid: palmitic acid, 2:1) for 24 h to stimulate in vitro fatty liver. In addition, C57BL/6 mice were fed 60 kcal% high-fat (HF) diet for eight weeks to induce fatty liver in vivo. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) productions were increased by FFA and HF-diet, but supplementation with CLW significantly decreased these levels. CLW treatment ameliorated antioxidant activities that were suppressed by exposure to the FFA and HF-diet. Cluster of differentiation 36 (CD36) and fatty acid transport proteins (FATP2 and FATP5) were increased in HF-diet groups, while CLW suppressed their expression levels. Moreover, sterol regulatory element-binding protein-1c (SREBP-1c), acetyl-coenzyme A carboxylase (ACC), and fatty acid synthase (FAS) expression levels were down-regulated in the CLW groups compared to HF-diet groups. On the other hand, 5′ adenosine monophosphate-activated protein kinase (AMPK), Peroxisome proliferator-activated receptor alpha (PPAR-α), and carnitine palmitoyltransferase 1 (CPT-1) expressions were up-regulated in the CLW groups. HF-diet fed mice showed high hepatic triglycerides (TG) content compared to the normal diet mice. However, the administration of CLW restored the hepatic TG level, indicating an inhibitory effect against lipid accumulation by CLW. These results suggest that CLW could be a potentially useful agent for the prevention of NAFLD through modulating fatty acid uptake.
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spelling pubmed-68355542019-11-25 Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease Mun, Jeongeun Kim, Shintae Yoon, Ho-Geun You, Yanghee Kim, Ok-Kyung Choi, Kyung-Chul Lee, Yoo-Hyun Lee, Jeongmin Park, Jeongjin Jun, Woojin Nutrients Article Our aim was to investigate whether hot water extract (CLW) of Curcuma longa L. could prevent non-alcoholic fatty liver disease (NAFLD). HepG2 cells were treated with free fatty acid (FFA) mixture (oleic acid: palmitic acid, 2:1) for 24 h to stimulate in vitro fatty liver. In addition, C57BL/6 mice were fed 60 kcal% high-fat (HF) diet for eight weeks to induce fatty liver in vivo. Intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) productions were increased by FFA and HF-diet, but supplementation with CLW significantly decreased these levels. CLW treatment ameliorated antioxidant activities that were suppressed by exposure to the FFA and HF-diet. Cluster of differentiation 36 (CD36) and fatty acid transport proteins (FATP2 and FATP5) were increased in HF-diet groups, while CLW suppressed their expression levels. Moreover, sterol regulatory element-binding protein-1c (SREBP-1c), acetyl-coenzyme A carboxylase (ACC), and fatty acid synthase (FAS) expression levels were down-regulated in the CLW groups compared to HF-diet groups. On the other hand, 5′ adenosine monophosphate-activated protein kinase (AMPK), Peroxisome proliferator-activated receptor alpha (PPAR-α), and carnitine palmitoyltransferase 1 (CPT-1) expressions were up-regulated in the CLW groups. HF-diet fed mice showed high hepatic triglycerides (TG) content compared to the normal diet mice. However, the administration of CLW restored the hepatic TG level, indicating an inhibitory effect against lipid accumulation by CLW. These results suggest that CLW could be a potentially useful agent for the prevention of NAFLD through modulating fatty acid uptake. MDPI 2019-10-21 /pmc/articles/PMC6835554/ /pubmed/31640183 http://dx.doi.org/10.3390/nu11102536 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mun, Jeongeun
Kim, Shintae
Yoon, Ho-Geun
You, Yanghee
Kim, Ok-Kyung
Choi, Kyung-Chul
Lee, Yoo-Hyun
Lee, Jeongmin
Park, Jeongjin
Jun, Woojin
Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title_full Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title_fullStr Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title_full_unstemmed Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title_short Water Extract of Curcuma longa L. Ameliorates Non-Alcoholic Fatty Liver Disease
title_sort water extract of curcuma longa l. ameliorates non-alcoholic fatty liver disease
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835554/
https://www.ncbi.nlm.nih.gov/pubmed/31640183
http://dx.doi.org/10.3390/nu11102536
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