Cargando…

A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1

In the desert locust Schistocerca gregaria (S. gregaria), pheromones are considered to be crucial for governing important behaviors and processes, including phase transition, reproduction, aggregation and swarm formation. The receptors mediating pheromone detection in olfactory sensory neurons (OSNs...

Descripción completa

Detalles Bibliográficos
Autores principales: Pregitzer, Pablo, Jiang, Xingcong, Lemke, René-Sebastian, Krieger, Jürgen, Fleischer, Jörg, Breer, Heinz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835626/
https://www.ncbi.nlm.nih.gov/pubmed/31627262
http://dx.doi.org/10.3390/insects10100350
_version_ 1783466716749103104
author Pregitzer, Pablo
Jiang, Xingcong
Lemke, René-Sebastian
Krieger, Jürgen
Fleischer, Jörg
Breer, Heinz
author_facet Pregitzer, Pablo
Jiang, Xingcong
Lemke, René-Sebastian
Krieger, Jürgen
Fleischer, Jörg
Breer, Heinz
author_sort Pregitzer, Pablo
collection PubMed
description In the desert locust Schistocerca gregaria (S. gregaria), pheromones are considered to be crucial for governing important behaviors and processes, including phase transition, reproduction, aggregation and swarm formation. The receptors mediating pheromone detection in olfactory sensory neurons (OSNs) on the antenna of S. gregaria are unknown. Since pheromone receptors in other insects belong to the odorant receptor (OR) family and are typically co-expressed with the “sensory neuron membrane protein 1” (SNMP1), in our search for putative pheromone receptors of S. gregaria, we have screened the OR repertoire for receptor types that are expressed in SNMP1-positive OSNs. Based on phylogenetic analyses, we categorized the 119 ORs of S. gregaria into three groups (I–III) and analyzed a substantial number of ORs for co-expression with SNMP1 by two-color fluorescence in situ hybridization. We have identified 33 ORs that were co-expressed with SNMP1. In fact, the majority of ORs from group I and II were found to be expressed in SNMP1-positive OSNs, but only very few receptors from group III, which comprises approximately 60% of all ORs from S. gregaria, were co-expressed with SNMP1. These findings indicate that numerous ORs from group I and II could be important for pheromone communication. Collectively, we have identified a broad range of candidate pheromone receptors in S. gregaria that are not randomly distributed throughout the OR family but rather segregate into phylogenetically distinct receptor clades.
format Online
Article
Text
id pubmed-6835626
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68356262019-11-25 A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1 Pregitzer, Pablo Jiang, Xingcong Lemke, René-Sebastian Krieger, Jürgen Fleischer, Jörg Breer, Heinz Insects Article In the desert locust Schistocerca gregaria (S. gregaria), pheromones are considered to be crucial for governing important behaviors and processes, including phase transition, reproduction, aggregation and swarm formation. The receptors mediating pheromone detection in olfactory sensory neurons (OSNs) on the antenna of S. gregaria are unknown. Since pheromone receptors in other insects belong to the odorant receptor (OR) family and are typically co-expressed with the “sensory neuron membrane protein 1” (SNMP1), in our search for putative pheromone receptors of S. gregaria, we have screened the OR repertoire for receptor types that are expressed in SNMP1-positive OSNs. Based on phylogenetic analyses, we categorized the 119 ORs of S. gregaria into three groups (I–III) and analyzed a substantial number of ORs for co-expression with SNMP1 by two-color fluorescence in situ hybridization. We have identified 33 ORs that were co-expressed with SNMP1. In fact, the majority of ORs from group I and II were found to be expressed in SNMP1-positive OSNs, but only very few receptors from group III, which comprises approximately 60% of all ORs from S. gregaria, were co-expressed with SNMP1. These findings indicate that numerous ORs from group I and II could be important for pheromone communication. Collectively, we have identified a broad range of candidate pheromone receptors in S. gregaria that are not randomly distributed throughout the OR family but rather segregate into phylogenetically distinct receptor clades. MDPI 2019-10-17 /pmc/articles/PMC6835626/ /pubmed/31627262 http://dx.doi.org/10.3390/insects10100350 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pregitzer, Pablo
Jiang, Xingcong
Lemke, René-Sebastian
Krieger, Jürgen
Fleischer, Jörg
Breer, Heinz
A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title_full A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title_fullStr A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title_full_unstemmed A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title_short A Subset of Odorant Receptors from the Desert Locust Schistocerca gregaria Is Co-Expressed with the Sensory Neuron Membrane Protein 1
title_sort subset of odorant receptors from the desert locust schistocerca gregaria is co-expressed with the sensory neuron membrane protein 1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835626/
https://www.ncbi.nlm.nih.gov/pubmed/31627262
http://dx.doi.org/10.3390/insects10100350
work_keys_str_mv AT pregitzerpablo asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT jiangxingcong asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT lemkerenesebastian asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT kriegerjurgen asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT fleischerjorg asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT breerheinz asubsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT pregitzerpablo subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT jiangxingcong subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT lemkerenesebastian subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT kriegerjurgen subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT fleischerjorg subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1
AT breerheinz subsetofodorantreceptorsfromthedesertlocustschistocercagregariaiscoexpressedwiththesensoryneuronmembraneprotein1