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Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species

Diseases associated with Xylella fastidiosa have been described mostly in North and South America. However, during the last five years, widespread X. fastidiosa infections have been reported in a constrained area of the Apulia region (southern Italy), in olives trees suffering a severe disease, deno...

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Autores principales: Cavalieri, Vincenzo, Altamura, Giuseppe, Fumarola, Giulio, di Carolo, Michele, Saponari, Maria, Cornara, Daniele, Bosco, Domenico, Dongiovanni, Crescenza
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835679/
https://www.ncbi.nlm.nih.gov/pubmed/31569480
http://dx.doi.org/10.3390/insects10100324
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author Cavalieri, Vincenzo
Altamura, Giuseppe
Fumarola, Giulio
di Carolo, Michele
Saponari, Maria
Cornara, Daniele
Bosco, Domenico
Dongiovanni, Crescenza
author_facet Cavalieri, Vincenzo
Altamura, Giuseppe
Fumarola, Giulio
di Carolo, Michele
Saponari, Maria
Cornara, Daniele
Bosco, Domenico
Dongiovanni, Crescenza
author_sort Cavalieri, Vincenzo
collection PubMed
description Diseases associated with Xylella fastidiosa have been described mostly in North and South America. However, during the last five years, widespread X. fastidiosa infections have been reported in a constrained area of the Apulia region (southern Italy), in olives trees suffering a severe disease, denoted as Olive Quick Decline Syndrome (OQDS). Because many xylem sap-feeding insects can function as vectors for the transmission of this exotic pathogen in EU, several research programs are ongoing to assess the role of candidate vectors in the spread of the infections. Initial investigations identified Philaenus spumarius (L.) as the predominant vector species in the olive orchards affected by the OQDS. Additional experiments have been carried out during 2016 and 2017 to assess the role of other species. More specifically, adults of the spittlebugs Philaenus italosignus Drosopolous and Remane, Neophilaenus campestris (Fallen) and of the planthopper Latilica tunetana (Matsumura) (Issidae) have been tested in transmission experiments to assess their ability to acquire the bacterium from infected olives and to infect different susceptible hosts (olives, almond, myrtle –leaf milkwort, periwinkle). Acquisition rates determined by testing individual insects in quantitative PCR assays, ranging from 5.6% in N. campestris to 22.2% in P. italosignus, whereas no acquisition was recorded for L. tunetana. Successful transmissions were detected in the recipient plants exposed to P. italosignus and N. campestris, whereas no trasmissions occurred with L. tunetana. The known vector Philaenus spumarius has been included in all the experiments for validation. The systematic surveys conducted in 2016 and 2017 provided further evidence on the population dynamics and seasonal abundance of the spittlebug populations in the olive groves.
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spelling pubmed-68356792019-11-25 Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species Cavalieri, Vincenzo Altamura, Giuseppe Fumarola, Giulio di Carolo, Michele Saponari, Maria Cornara, Daniele Bosco, Domenico Dongiovanni, Crescenza Insects Article Diseases associated with Xylella fastidiosa have been described mostly in North and South America. However, during the last five years, widespread X. fastidiosa infections have been reported in a constrained area of the Apulia region (southern Italy), in olives trees suffering a severe disease, denoted as Olive Quick Decline Syndrome (OQDS). Because many xylem sap-feeding insects can function as vectors for the transmission of this exotic pathogen in EU, several research programs are ongoing to assess the role of candidate vectors in the spread of the infections. Initial investigations identified Philaenus spumarius (L.) as the predominant vector species in the olive orchards affected by the OQDS. Additional experiments have been carried out during 2016 and 2017 to assess the role of other species. More specifically, adults of the spittlebugs Philaenus italosignus Drosopolous and Remane, Neophilaenus campestris (Fallen) and of the planthopper Latilica tunetana (Matsumura) (Issidae) have been tested in transmission experiments to assess their ability to acquire the bacterium from infected olives and to infect different susceptible hosts (olives, almond, myrtle –leaf milkwort, periwinkle). Acquisition rates determined by testing individual insects in quantitative PCR assays, ranging from 5.6% in N. campestris to 22.2% in P. italosignus, whereas no acquisition was recorded for L. tunetana. Successful transmissions were detected in the recipient plants exposed to P. italosignus and N. campestris, whereas no trasmissions occurred with L. tunetana. The known vector Philaenus spumarius has been included in all the experiments for validation. The systematic surveys conducted in 2016 and 2017 provided further evidence on the population dynamics and seasonal abundance of the spittlebug populations in the olive groves. MDPI 2019-09-29 /pmc/articles/PMC6835679/ /pubmed/31569480 http://dx.doi.org/10.3390/insects10100324 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Cavalieri, Vincenzo
Altamura, Giuseppe
Fumarola, Giulio
di Carolo, Michele
Saponari, Maria
Cornara, Daniele
Bosco, Domenico
Dongiovanni, Crescenza
Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title_full Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title_fullStr Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title_full_unstemmed Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title_short Transmission of Xylella fastidiosa Subspecies Pauca Sequence Type 53 by Different Insect Species
title_sort transmission of xylella fastidiosa subspecies pauca sequence type 53 by different insect species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835679/
https://www.ncbi.nlm.nih.gov/pubmed/31569480
http://dx.doi.org/10.3390/insects10100324
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