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Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model

Food proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and aller...

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Autores principales: Ondracek, Anna S., Heiden, Denise, Oostingh, Gertie J., Fuerst, Elisabeth, Fazekas-Singer, Judit, Bergmayr, Cornelia, Rohrhofer, Johanna, Jensen-Jarolim, Erika, Duschl, Albert, Untersmayr, Eva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835712/
https://www.ncbi.nlm.nih.gov/pubmed/31618852
http://dx.doi.org/10.3390/nu11102463
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author Ondracek, Anna S.
Heiden, Denise
Oostingh, Gertie J.
Fuerst, Elisabeth
Fazekas-Singer, Judit
Bergmayr, Cornelia
Rohrhofer, Johanna
Jensen-Jarolim, Erika
Duschl, Albert
Untersmayr, Eva
author_facet Ondracek, Anna S.
Heiden, Denise
Oostingh, Gertie J.
Fuerst, Elisabeth
Fazekas-Singer, Judit
Bergmayr, Cornelia
Rohrhofer, Johanna
Jensen-Jarolim, Erika
Duschl, Albert
Untersmayr, Eva
author_sort Ondracek, Anna S.
collection PubMed
description Food proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and allergic mice using the milk allergen beta-lactoglobulin (BLG) as model food protein in a mouse model. Evaluating the presence of nitrated proteins in food, we could detect 3-nitrotyrosine (3-NT) in extracts of different foods and in stomach content extracts of non-allergic mice under physiological conditions. Chemically nitrated BLG (BLGn) exhibited enhanced susceptibility to degradation in simulated gastric fluid experiments compared to untreated BLG (BLGu). Gavage of BLGn to non-allergic animals increased interferon-γ and interleukin-10 release of stimulated spleen cells and led to the formation of BLG-specific serum IgA. Allergic mice receiving three oral gavages of BLGn had higher levels of mouse mast cell protease-1 (mMCP-1) compared to allergic mice receiving BLGu. Regardless of the preceding immune status, non-allergic or allergic, repeatedly ingested nitrated food proteins seem to considerably influence the subsequent immune response.
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spelling pubmed-68357122019-11-25 Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model Ondracek, Anna S. Heiden, Denise Oostingh, Gertie J. Fuerst, Elisabeth Fazekas-Singer, Judit Bergmayr, Cornelia Rohrhofer, Johanna Jensen-Jarolim, Erika Duschl, Albert Untersmayr, Eva Nutrients Article Food proteins may get nitrated by various exogenous or endogenous mechanisms. As individuals might get recurrently exposed to nitrated proteins via daily diet, we aimed to investigate the effect of repeatedly ingested nitrated food proteins on the subsequent immune response in non-allergic and allergic mice using the milk allergen beta-lactoglobulin (BLG) as model food protein in a mouse model. Evaluating the presence of nitrated proteins in food, we could detect 3-nitrotyrosine (3-NT) in extracts of different foods and in stomach content extracts of non-allergic mice under physiological conditions. Chemically nitrated BLG (BLGn) exhibited enhanced susceptibility to degradation in simulated gastric fluid experiments compared to untreated BLG (BLGu). Gavage of BLGn to non-allergic animals increased interferon-γ and interleukin-10 release of stimulated spleen cells and led to the formation of BLG-specific serum IgA. Allergic mice receiving three oral gavages of BLGn had higher levels of mouse mast cell protease-1 (mMCP-1) compared to allergic mice receiving BLGu. Regardless of the preceding immune status, non-allergic or allergic, repeatedly ingested nitrated food proteins seem to considerably influence the subsequent immune response. MDPI 2019-10-15 /pmc/articles/PMC6835712/ /pubmed/31618852 http://dx.doi.org/10.3390/nu11102463 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ondracek, Anna S.
Heiden, Denise
Oostingh, Gertie J.
Fuerst, Elisabeth
Fazekas-Singer, Judit
Bergmayr, Cornelia
Rohrhofer, Johanna
Jensen-Jarolim, Erika
Duschl, Albert
Untersmayr, Eva
Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title_full Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title_fullStr Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title_full_unstemmed Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title_short Immune Effects of the Nitrated Food Allergen Beta-Lactoglobulin in an Experimental Food Allergy Model
title_sort immune effects of the nitrated food allergen beta-lactoglobulin in an experimental food allergy model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835712/
https://www.ncbi.nlm.nih.gov/pubmed/31618852
http://dx.doi.org/10.3390/nu11102463
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