Cargando…

Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets

Nanotechnology is being increasingly utilised in medicine as diagnostics and for drug delivery and targeting. The small size and high surface area of nanoparticles (NPs), desirable properties that allow them to cross biological barriers, also offer potential for interaction with other cells and bloo...

Descripción completa

Detalles Bibliográficos
Autores principales: Bakhaidar, Rana, Green, Joshua, Alfahad, Khaled, Samanani, Shazia, Moollan, Nabeehah, O’Neill, Sarah, Ramtoola, Zebunnissa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835715/
https://www.ncbi.nlm.nih.gov/pubmed/31590303
http://dx.doi.org/10.3390/pharmaceutics11100514
_version_ 1783466737990107136
author Bakhaidar, Rana
Green, Joshua
Alfahad, Khaled
Samanani, Shazia
Moollan, Nabeehah
O’Neill, Sarah
Ramtoola, Zebunnissa
author_facet Bakhaidar, Rana
Green, Joshua
Alfahad, Khaled
Samanani, Shazia
Moollan, Nabeehah
O’Neill, Sarah
Ramtoola, Zebunnissa
author_sort Bakhaidar, Rana
collection PubMed
description Nanotechnology is being increasingly utilised in medicine as diagnostics and for drug delivery and targeting. The small size and high surface area of nanoparticles (NPs), desirable properties that allow them to cross biological barriers, also offer potential for interaction with other cells and blood constituents, presenting possible safety risks. While NPs investigated are predominantly based on the biodegradable, biocompatible, and FDA approved poly-lactide-co-glycolide (PLGA) polymers, pro-aggregatory and antiplatelet effects have been reported for certain NPs. The potential for toxicity of PLGA based NPs remains to be examined. The aims of this study were to determine the impact of size-selected PLGA-PEG (PLGA-polyethylene glycol) NPs on platelet activation and aggregation. PLGA-PEG NPs of three average sizes of 112, 348, and 576 nm were formulated and their effect at concentrations of 0.0–2.2 mg/mL on the activation and aggregation of washed human platelets (WP) was examined. The results of this study show, for the first time, NPs of all sizes associated with the surface of platelets, with >50% binding, leading to possible internalisation. The NP-platelet interaction, however, did not lead to platelet aggregation nor inhibited aggregation of platelets induced by thrombin. The outcome of this study is promising, suggesting that these NPs could be potential carriers for targeted drug delivery to platelets.
format Online
Article
Text
id pubmed-6835715
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68357152019-11-25 Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets Bakhaidar, Rana Green, Joshua Alfahad, Khaled Samanani, Shazia Moollan, Nabeehah O’Neill, Sarah Ramtoola, Zebunnissa Pharmaceutics Article Nanotechnology is being increasingly utilised in medicine as diagnostics and for drug delivery and targeting. The small size and high surface area of nanoparticles (NPs), desirable properties that allow them to cross biological barriers, also offer potential for interaction with other cells and blood constituents, presenting possible safety risks. While NPs investigated are predominantly based on the biodegradable, biocompatible, and FDA approved poly-lactide-co-glycolide (PLGA) polymers, pro-aggregatory and antiplatelet effects have been reported for certain NPs. The potential for toxicity of PLGA based NPs remains to be examined. The aims of this study were to determine the impact of size-selected PLGA-PEG (PLGA-polyethylene glycol) NPs on platelet activation and aggregation. PLGA-PEG NPs of three average sizes of 112, 348, and 576 nm were formulated and their effect at concentrations of 0.0–2.2 mg/mL on the activation and aggregation of washed human platelets (WP) was examined. The results of this study show, for the first time, NPs of all sizes associated with the surface of platelets, with >50% binding, leading to possible internalisation. The NP-platelet interaction, however, did not lead to platelet aggregation nor inhibited aggregation of platelets induced by thrombin. The outcome of this study is promising, suggesting that these NPs could be potential carriers for targeted drug delivery to platelets. MDPI 2019-10-04 /pmc/articles/PMC6835715/ /pubmed/31590303 http://dx.doi.org/10.3390/pharmaceutics11100514 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bakhaidar, Rana
Green, Joshua
Alfahad, Khaled
Samanani, Shazia
Moollan, Nabeehah
O’Neill, Sarah
Ramtoola, Zebunnissa
Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title_full Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title_fullStr Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title_full_unstemmed Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title_short Effect of Size and Concentration of PLGA-PEG Nanoparticles on Activation and Aggregation of Washed Human Platelets
title_sort effect of size and concentration of plga-peg nanoparticles on activation and aggregation of washed human platelets
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835715/
https://www.ncbi.nlm.nih.gov/pubmed/31590303
http://dx.doi.org/10.3390/pharmaceutics11100514
work_keys_str_mv AT bakhaidarrana effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT greenjoshua effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT alfahadkhaled effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT samananishazia effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT moollannabeehah effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT oneillsarah effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets
AT ramtoolazebunnissa effectofsizeandconcentrationofplgapegnanoparticlesonactivationandaggregationofwashedhumanplatelets