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Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings
As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings. Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healin...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835814/ https://www.ncbi.nlm.nih.gov/pubmed/31614468 http://dx.doi.org/10.3390/md17100575 |
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author | Gao, Yingjun Zhang, Xing Jin, Xiangyu |
author_facet | Gao, Yingjun Zhang, Xing Jin, Xiangyu |
author_sort | Gao, Yingjun |
collection | PubMed |
description | As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings. Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing. Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application. In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking. The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates. Skin-like tensile performance allowed the dressings to provide a better healing environment. Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings. The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time. Cell culture assay showed that the dressings had excellent cell biocompatibility. As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds. |
format | Online Article Text |
id | pubmed-6835814 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68358142019-11-25 Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings Gao, Yingjun Zhang, Xing Jin, Xiangyu Mar Drugs Article As derivatives from marine natural biomaterials, alginate-based and chitosan-based biomaterials are commonly used in wound dressings. Calcium alginate fiber (CAF) dressings possess excellent absorption and unique gel forming performance, but the low bioactivity limits its application in wound healing. Carboxymethyl chitosan (CM-Chit) has excellent antibacterial activity, but the gel structure with weak mechanical properties restricts its application. In this study, minocycline (Mino)/CM-Chit solution was coated on the surface of plasma treated CAF needle-punched nonwovens, and then Mino loaded CM-Chit gel/CAF nonwovens composite dressings were fabricated by EDC/NHS (1-3-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride/N-hydroxysuccinimide) crosslinking. The dressings had a porous composite structure, which allowed them to quickly absorb and store a large number of wound exudates. Skin-like tensile performance allowed the dressings to provide a better healing environment. Antibacterial assay against Escherichia coli and Staphylococcus aureus indicated that the addition of Mino significantly improved the antibacterial activity of the wound dressings. The tight structure of CM-Chit gel prevented the burst release of Mino so that the dressings had antibacterial activity in a certain period of release time. Cell culture assay showed that the dressings had excellent cell biocompatibility. As new functional dressings, the prepared composite dressings had excellent potential in the clinical healing of wounds. MDPI 2019-10-11 /pmc/articles/PMC6835814/ /pubmed/31614468 http://dx.doi.org/10.3390/md17100575 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gao, Yingjun Zhang, Xing Jin, Xiangyu Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title | Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title_full | Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title_fullStr | Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title_full_unstemmed | Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title_short | Preparation and Properties of Minocycline-Loaded Carboxymethyl Chitosan Gel/Alginate Nonwovens Composite Wound Dressings |
title_sort | preparation and properties of minocycline-loaded carboxymethyl chitosan gel/alginate nonwovens composite wound dressings |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835814/ https://www.ncbi.nlm.nih.gov/pubmed/31614468 http://dx.doi.org/10.3390/md17100575 |
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