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Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin

Collagen is widely used in the pharmaceutical, tissue engineering, nutraceutical, and cosmetic industries. In this study, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from the skin of red stingray, and its physicochemical and functional properties were investigated. T...

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Autores principales: Chen, Junde, Li, Jianying, Li, Zhongbao, Yi, Ruizao, Shi, Shenjia, Wu, Kunyuan, Li, Yushuang, Wu, Sijia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835876/
https://www.ncbi.nlm.nih.gov/pubmed/31569390
http://dx.doi.org/10.3390/md17100558
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author Chen, Junde
Li, Jianying
Li, Zhongbao
Yi, Ruizao
Shi, Shenjia
Wu, Kunyuan
Li, Yushuang
Wu, Sijia
author_facet Chen, Junde
Li, Jianying
Li, Zhongbao
Yi, Ruizao
Shi, Shenjia
Wu, Kunyuan
Li, Yushuang
Wu, Sijia
author_sort Chen, Junde
collection PubMed
description Collagen is widely used in the pharmaceutical, tissue engineering, nutraceutical, and cosmetic industries. In this study, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from the skin of red stingray, and its physicochemical and functional properties were investigated. The yields of ASC and PSC were 33.95 ± 0.7% and 37.18 ± 0.71% (on a dry weight basis), respectively. ASC and PSC were identified as type I collagen by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis, possessing a complete triple helix structure as determined by UV absorption, Fourier transform infrared, circular dichroism, and X-ray diffraction spectroscopy. Contact angle experiments indicated that PSC was more hydrophobic than ASC. Thermal stability tests revealed that the melting temperature of PSC from red stingray skin was higher than that of PSC from duck skin, and the difference in the melting temperature between these two PSCs was 9.24 °C. Additionally, both ASC and PSC were functionally superior to some other proteins from terrestrial sources, such as scallop gonad protein, whey protein, and goose liver protein. These results suggest that PSC from red stingray skin could be used instead of terrestrial animal collagen in drugs, foods, cosmetics, and biological functional materials, and as scaffolds for bone regeneration.
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spelling pubmed-68358762019-11-25 Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin Chen, Junde Li, Jianying Li, Zhongbao Yi, Ruizao Shi, Shenjia Wu, Kunyuan Li, Yushuang Wu, Sijia Mar Drugs Article Collagen is widely used in the pharmaceutical, tissue engineering, nutraceutical, and cosmetic industries. In this study, acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted from the skin of red stingray, and its physicochemical and functional properties were investigated. The yields of ASC and PSC were 33.95 ± 0.7% and 37.18 ± 0.71% (on a dry weight basis), respectively. ASC and PSC were identified as type I collagen by Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) analysis, possessing a complete triple helix structure as determined by UV absorption, Fourier transform infrared, circular dichroism, and X-ray diffraction spectroscopy. Contact angle experiments indicated that PSC was more hydrophobic than ASC. Thermal stability tests revealed that the melting temperature of PSC from red stingray skin was higher than that of PSC from duck skin, and the difference in the melting temperature between these two PSCs was 9.24 °C. Additionally, both ASC and PSC were functionally superior to some other proteins from terrestrial sources, such as scallop gonad protein, whey protein, and goose liver protein. These results suggest that PSC from red stingray skin could be used instead of terrestrial animal collagen in drugs, foods, cosmetics, and biological functional materials, and as scaffolds for bone regeneration. MDPI 2019-09-28 /pmc/articles/PMC6835876/ /pubmed/31569390 http://dx.doi.org/10.3390/md17100558 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Junde
Li, Jianying
Li, Zhongbao
Yi, Ruizao
Shi, Shenjia
Wu, Kunyuan
Li, Yushuang
Wu, Sijia
Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title_full Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title_fullStr Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title_full_unstemmed Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title_short Physicochemical and Functional Properties of Type I Collagens in Red Stingray (Dasyatis akajei) Skin
title_sort physicochemical and functional properties of type i collagens in red stingray (dasyatis akajei) skin
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835876/
https://www.ncbi.nlm.nih.gov/pubmed/31569390
http://dx.doi.org/10.3390/md17100558
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