Cargando…

The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves

In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential....

Descripción completa

Detalles Bibliográficos
Autores principales: Hernández-Bolio, Gloria I., Dzul-Romero, Rubí E., Maldonado Velázquez, María G., Zamora Cresencio, Pedro, Hernández-Núñez, Emanuel, Aguirre-Crespo, Francisco J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835937/
https://www.ncbi.nlm.nih.gov/pubmed/31590430
http://dx.doi.org/10.3390/metabo9100217
_version_ 1783466791117258752
author Hernández-Bolio, Gloria I.
Dzul-Romero, Rubí E.
Maldonado Velázquez, María G.
Zamora Cresencio, Pedro
Hernández-Núñez, Emanuel
Aguirre-Crespo, Francisco J.
author_facet Hernández-Bolio, Gloria I.
Dzul-Romero, Rubí E.
Maldonado Velázquez, María G.
Zamora Cresencio, Pedro
Hernández-Núñez, Emanuel
Aguirre-Crespo, Francisco J.
author_sort Hernández-Bolio, Gloria I.
collection PubMed
description In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential. For this purpose, a methanol extraction of leaves dried at room temperature (25 °C) or by heat convection (50, 75 and 100 °C) were compared in terms of drying efficiency, yield of extraction, total phenol content, (1)H-NMR metabolic profile, and DPPH antioxidant activity. The drying curves enabled the fact to be uncovered that drying efficiency improves with increase of temperature, as does the level of total phenols and antioxidant activity. A metabolomics approach using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the corresponding (1)H-NMR profiles allowed the impact of the drying temperature on their metabolic profile to be documented and also, caffeic acid and epicatechin as main secondary metabolites contributing to the antioxidant activity of M. zapota to be identified.
format Online
Article
Text
id pubmed-6835937
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-68359372019-11-25 The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves Hernández-Bolio, Gloria I. Dzul-Romero, Rubí E. Maldonado Velázquez, María G. Zamora Cresencio, Pedro Hernández-Núñez, Emanuel Aguirre-Crespo, Francisco J. Metabolites Article In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential. For this purpose, a methanol extraction of leaves dried at room temperature (25 °C) or by heat convection (50, 75 and 100 °C) were compared in terms of drying efficiency, yield of extraction, total phenol content, (1)H-NMR metabolic profile, and DPPH antioxidant activity. The drying curves enabled the fact to be uncovered that drying efficiency improves with increase of temperature, as does the level of total phenols and antioxidant activity. A metabolomics approach using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the corresponding (1)H-NMR profiles allowed the impact of the drying temperature on their metabolic profile to be documented and also, caffeic acid and epicatechin as main secondary metabolites contributing to the antioxidant activity of M. zapota to be identified. MDPI 2019-10-06 /pmc/articles/PMC6835937/ /pubmed/31590430 http://dx.doi.org/10.3390/metabo9100217 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hernández-Bolio, Gloria I.
Dzul-Romero, Rubí E.
Maldonado Velázquez, María G.
Zamora Cresencio, Pedro
Hernández-Núñez, Emanuel
Aguirre-Crespo, Francisco J.
The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title_full The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title_fullStr The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title_full_unstemmed The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title_short The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
title_sort influence of drying temperatures on the metabolic profiles and antioxidant activity of manilkara zapota leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835937/
https://www.ncbi.nlm.nih.gov/pubmed/31590430
http://dx.doi.org/10.3390/metabo9100217
work_keys_str_mv AT hernandezboliogloriai theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT dzulromerorubie theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT maldonadovelazquezmariag theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT zamoracresenciopedro theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT hernandeznunezemanuel theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT aguirrecrespofranciscoj theinfluenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT hernandezboliogloriai influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT dzulromerorubie influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT maldonadovelazquezmariag influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT zamoracresenciopedro influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT hernandeznunezemanuel influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves
AT aguirrecrespofranciscoj influenceofdryingtemperaturesonthemetabolicprofilesandantioxidantactivityofmanilkarazapotaleaves