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The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves
In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential....
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835937/ https://www.ncbi.nlm.nih.gov/pubmed/31590430 http://dx.doi.org/10.3390/metabo9100217 |
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author | Hernández-Bolio, Gloria I. Dzul-Romero, Rubí E. Maldonado Velázquez, María G. Zamora Cresencio, Pedro Hernández-Núñez, Emanuel Aguirre-Crespo, Francisco J. |
author_facet | Hernández-Bolio, Gloria I. Dzul-Romero, Rubí E. Maldonado Velázquez, María G. Zamora Cresencio, Pedro Hernández-Núñez, Emanuel Aguirre-Crespo, Francisco J. |
author_sort | Hernández-Bolio, Gloria I. |
collection | PubMed |
description | In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential. For this purpose, a methanol extraction of leaves dried at room temperature (25 °C) or by heat convection (50, 75 and 100 °C) were compared in terms of drying efficiency, yield of extraction, total phenol content, (1)H-NMR metabolic profile, and DPPH antioxidant activity. The drying curves enabled the fact to be uncovered that drying efficiency improves with increase of temperature, as does the level of total phenols and antioxidant activity. A metabolomics approach using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the corresponding (1)H-NMR profiles allowed the impact of the drying temperature on their metabolic profile to be documented and also, caffeic acid and epicatechin as main secondary metabolites contributing to the antioxidant activity of M. zapota to be identified. |
format | Online Article Text |
id | pubmed-6835937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68359372019-11-25 The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves Hernández-Bolio, Gloria I. Dzul-Romero, Rubí E. Maldonado Velázquez, María G. Zamora Cresencio, Pedro Hernández-Núñez, Emanuel Aguirre-Crespo, Francisco J. Metabolites Article In the present study, the leaves of Manilkara zapota (L.) P. Royen (Sapotaceae), an evergreen tree recognized for its medicinal properties in Southern Mexico, were used as a model to study the effect of different drying temperatures on its metabolic profile and therefore, its antioxidant potential. For this purpose, a methanol extraction of leaves dried at room temperature (25 °C) or by heat convection (50, 75 and 100 °C) were compared in terms of drying efficiency, yield of extraction, total phenol content, (1)H-NMR metabolic profile, and DPPH antioxidant activity. The drying curves enabled the fact to be uncovered that drying efficiency improves with increase of temperature, as does the level of total phenols and antioxidant activity. A metabolomics approach using principal component analysis (PCA) and orthogonal projections to latent structures discriminant analysis (OPLS-DA) of the corresponding (1)H-NMR profiles allowed the impact of the drying temperature on their metabolic profile to be documented and also, caffeic acid and epicatechin as main secondary metabolites contributing to the antioxidant activity of M. zapota to be identified. MDPI 2019-10-06 /pmc/articles/PMC6835937/ /pubmed/31590430 http://dx.doi.org/10.3390/metabo9100217 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hernández-Bolio, Gloria I. Dzul-Romero, Rubí E. Maldonado Velázquez, María G. Zamora Cresencio, Pedro Hernández-Núñez, Emanuel Aguirre-Crespo, Francisco J. The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title | The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title_full | The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title_fullStr | The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title_full_unstemmed | The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title_short | The Influence of Drying Temperatures on the Metabolic Profiles and Antioxidant Activity of Manilkara zapota Leaves |
title_sort | influence of drying temperatures on the metabolic profiles and antioxidant activity of manilkara zapota leaves |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6835937/ https://www.ncbi.nlm.nih.gov/pubmed/31590430 http://dx.doi.org/10.3390/metabo9100217 |
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