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Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836040/ https://www.ncbi.nlm.nih.gov/pubmed/31581730 http://dx.doi.org/10.3390/nano9101407 |
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author | Parisi, Ludovica Toffoli, Andrea Cutrera, Miriam Bianchi, Massimiliano G. Lumetti, Simone Bussolati, Ovidio Macaluso, Guido M. |
author_facet | Parisi, Ludovica Toffoli, Andrea Cutrera, Miriam Bianchi, Massimiliano G. Lumetti, Simone Bussolati, Ovidio Macaluso, Guido M. |
author_sort | Parisi, Ludovica |
collection | PubMed |
description | The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression. |
format | Online Article Text |
id | pubmed-6836040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-68360402019-11-25 Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization Parisi, Ludovica Toffoli, Andrea Cutrera, Miriam Bianchi, Massimiliano G. Lumetti, Simone Bussolati, Ovidio Macaluso, Guido M. Nanomaterials (Basel) Article The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression. MDPI 2019-10-02 /pmc/articles/PMC6836040/ /pubmed/31581730 http://dx.doi.org/10.3390/nano9101407 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Parisi, Ludovica Toffoli, Andrea Cutrera, Miriam Bianchi, Massimiliano G. Lumetti, Simone Bussolati, Ovidio Macaluso, Guido M. Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title | Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title_full | Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title_fullStr | Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title_full_unstemmed | Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title_short | Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization |
title_sort | plasma proteins at the interface of dental implants modulate osteoblasts focal adhesions expression and cytoskeleton organization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836040/ https://www.ncbi.nlm.nih.gov/pubmed/31581730 http://dx.doi.org/10.3390/nano9101407 |
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