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Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization

The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after...

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Autores principales: Parisi, Ludovica, Toffoli, Andrea, Cutrera, Miriam, Bianchi, Massimiliano G., Lumetti, Simone, Bussolati, Ovidio, Macaluso, Guido M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836040/
https://www.ncbi.nlm.nih.gov/pubmed/31581730
http://dx.doi.org/10.3390/nano9101407
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author Parisi, Ludovica
Toffoli, Andrea
Cutrera, Miriam
Bianchi, Massimiliano G.
Lumetti, Simone
Bussolati, Ovidio
Macaluso, Guido M.
author_facet Parisi, Ludovica
Toffoli, Andrea
Cutrera, Miriam
Bianchi, Massimiliano G.
Lumetti, Simone
Bussolati, Ovidio
Macaluso, Guido M.
author_sort Parisi, Ludovica
collection PubMed
description The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression.
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spelling pubmed-68360402019-11-25 Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization Parisi, Ludovica Toffoli, Andrea Cutrera, Miriam Bianchi, Massimiliano G. Lumetti, Simone Bussolati, Ovidio Macaluso, Guido M. Nanomaterials (Basel) Article The host-material interface is a crucial relationship dictating the possibility of successful osseointegration in implant dentistry. The aim of the present study was to characterize the effects of plasma proteins pre-adsorption on the adhesion capacity of osteoblasts, which occurs immediately after implant insertion in vivo. After having pre-adsorbed human plasma proteins on a machined and microrough titanium surface, MC3T3-E1 osteoblasts adhesion was evaluated through crystal violet cell adhesion assay, immunofluorescence staining for cytoskeleton, focal adhesions and cell nuclei, and scanning electron microscopy. The pre-adsorbed protein layer markedly affected the adhesion rate of cells, as well as their morphology and the expression of focal contacts. Moreover, protein adsorption to the underlying titanium surface was found to be correlated to surface pre-wetting. Thus, the early adsorption of serum proteins to the interface of dental implants impacts cell adhesion in terms of strength and of focal adhesions expression. MDPI 2019-10-02 /pmc/articles/PMC6836040/ /pubmed/31581730 http://dx.doi.org/10.3390/nano9101407 Text en © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Parisi, Ludovica
Toffoli, Andrea
Cutrera, Miriam
Bianchi, Massimiliano G.
Lumetti, Simone
Bussolati, Ovidio
Macaluso, Guido M.
Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title_full Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title_fullStr Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title_full_unstemmed Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title_short Plasma Proteins at the Interface of Dental Implants Modulate Osteoblasts Focal Adhesions Expression and Cytoskeleton Organization
title_sort plasma proteins at the interface of dental implants modulate osteoblasts focal adhesions expression and cytoskeleton organization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6836040/
https://www.ncbi.nlm.nih.gov/pubmed/31581730
http://dx.doi.org/10.3390/nano9101407
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